NDUFB5 Antibody (F-2): sc-514245

NDUFB5 Antibody (F-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects NDUFB5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NDUFB5 (F-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NDUFB5, also known as NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5, plays a crucial role as an accessory subunit of Complex I, which is a vital component of the mitochondrial electron transport chain. Complex I catalyzes the transfer of electrons from NADH to ubiquinone, a process essential for cellular respiration and energy production. Complex I′s proper functioning is critical, as Complex I not only facilitates the conversion of energy stored in nutrients into ATP but also helps maintain the electrochemical gradient necessary for various cellular processes. NDUFB5 is a single-pass membrane protein located in the mitochondrial inner membrane, and NDUFB5′s interactions with other subunits of Complex I are essential for the stability and activity of the entire complex, highlighting NDUFB5′s importance in mitochondrial function and overall cellular health.

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NDUFB5 Antibody (F-2) References:

1. The nuclear encoded subunits of complex I from bovine heart mitochondria.  |  Hirst, J., et al. 2003. Biochim Biophys Acta. 1604: 135-50. PMID: 12837546
2. A high-fat diet coordinately downregulates genes required for mitochondrial oxidative phosphorylation in skeletal muscle.  |  Sparks, LM., et al. 2005. Diabetes. 54: 1926-33. PMID: 15983191
3. Mitochondrial complex I: structure, function and pathology.  |  Janssen, RJ., et al. 2006. J Inherit Metab Dis. 29: 499-515. PMID: 16838076
4. Adenosine 3',5'-cyclic monophosphate (cAMP)-dependent phosphoregulation of mitochondrial complex I is inhibited by nucleoside reverse transcriptase inhibitors.  |  Lund, KC. and Wallace, KB. 2008. Toxicol Appl Pharmacol. 226: 94-106. PMID: 17904600
5. cAMP-dependent protein kinase regulates the mitochondrial import of the nuclear encoded NDUFS4 subunit of complex I.  |  De Rasmo, D., et al. 2008. Cell Signal. 20: 989-97. PMID: 18291624
6. Molecular correlates for maximal oxygen uptake and type 1 fibers.  |  Parikh, H., et al. 2008. Am J Physiol Endocrinol Metab. 294: E1152-9. PMID: 18445752
7. Potentially Critical Roles of NDUFB5, TIMMDC1, and VDAC3 in the Progression of Septic Cardiomyopathy Through Integrated Bioinformatics Analysis.  |  Kang, K., et al. 2020. DNA Cell Biol. 39: 105-117. PMID: 31794266
8. Metabolic effects of RUBCN/Rubicon deficiency in kidney proximal tubular epithelial cells.  |  Matsuda, J., et al. 2020. Autophagy. 16: 1889-1904. PMID: 31944172
9. OMA1 reprograms metabolism under hypoxia to promote colorectal cancer development.  |  Wu, Z., et al. 2021. EMBO Rep. 22: e50827. PMID: 33314701
10. Proteomic signatures of infiltrative gastric cancer by proteomic and bioinformatic analysis.  |  Zhang, LH., et al. 2022. World J Gastrointest Oncol. 14: 2097-2107. PMID: 36438703
11. METTL3-mediated NDUFB5 m6A modification promotes cell migration and mitochondrial respiration to promote the wound healing of diabetic foot ulcer.  |  Wang, T., et al. 2024. J Transl Med. 22: 643. PMID: 38982516
12. Identification and primary structure of five human NADH-ubiquinone oxidoreductase subunits.  |  Ton, C., et al. 1997. Biochem Biophys Res Commun. 241: 589-94. PMID: 9425316

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NDUFB5 Antibody (F-2) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-NDUFB5 Antibody (F-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NDUFB5 Antibody (F-2) Specifications

Alternate Product Name: NDUFB5

Product Clone Name: F-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 78-131

Epitope Target: NDUFB5

Epitope Species: human

Product Reactivity/Detection: NDUFB5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for NDUFB5 Antibody (F-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin