Date published: 2025-9-7

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N-Ras 항체 (F155): sc-31

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데이터 시트
  • 안티-N-Ras 항체(F155) 는 마우스 monoclonal IgG1 κ N-Ras 항체, 240간행물에 인용, 이며 100 µg/ml으로 제공합니다.
  • human origin의 N-Ras p21의 전체인 아미노산을 항원으로 사용하였습니다.
  • WB, IP, IF와 IHC(P)으로 mouse, rat와 human origin의 N-Ras p21을 검출할것을 권장합니다.
  • 항-N-Ras 항체(F155)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • N-Ras (F155): sc-31무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 는 N-Ras 항체 (F155) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은N-Ras 항체(F155) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다).

빠른 링크

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N-Ras 항체 (F155)는 WB, IP, IF 및 IHC(P)에 의해 마우스, 랫트 및 인간 출처의 N-Ras 단백질을 검출하는 IgG1 마우스 단일 클론 N-Ras 항체 (또한 NRAS 항체로 지정됨)이다. N-Ras 항체 (F155)는 비-접합 항-N-Ras 항체로 사용될 수 있다. 포유류 Ras (v-Ha-Ras, Harvey rat 육종 바이러스 종양 유전자 동족체, HRAS1, K-Ras, N-Ras, RAS1 또는 c-bas/has로도 지정됨) 유전자 계열은 각각 (c-H-Ras2 및 c-K-Ras1)의 비활성 유사 유전자인 Harvey 및 Kirsten Ras 유전자 (c-H-Ras1 및 c-K-Ras2)로 구성된다. 세 개의 Ras 종양 유전자인 H-Ras, K-Ras 및 N-Ras는 GTP/GDP 결합 및 GTPase 활성을 갖는 단백질을 인코딩한다. Ras 단백질은 GDP에 결합된 비활성 형태와 GTP에 결합된 활성 형태 사이에서 번갈아 가며, 구아닌 뉴클레오티드 교환 인자 (GEF)에 의해 활성화되고 GTPase 활성 단백질 (GAP)에 의해 비활성화된다. Ras 명명법은 쥐에서 유래한 쥐 육종 바이러스 (HaMSV)의 하비 균주인 C형 포유류 레트로바이러스의 발암 서열을 포함하는 복제된 DNA 단편과 상동인 인간 DNA 서열의 특성에서 유래한다. 정상적인 조건에서, Ras 계열 구성체는 세포하막 구획화 기반 신호 시스템에서 세포 성장 및 분화 이벤트에 영향을 미친다. 발암형 Ras는 세포 증식과 세포 자멸을 모두 제어하는 프로세스를 탈규제할 수 있다. GTP 가수분해 결합 신호 전달 릴레이 단백질의 Ras 슈퍼 계열은 Ras, Rho, Rab 및 ARF 계열로 하위 분류될 수 있다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

N-Ras 항체 (F155) 참고문헌:

  1. Ras의 어두운 면: 세포사멸 조절.  |  Cox, AD. and Der, CJ. 2003. Oncogene. 22: 8999-9006. PMID: 14663478
  2. 전립선암 진행에서의 Ras 신호.  |  Weber, MJ. and Gioeli, D. 2004. J Cell Biochem. 91: 13-25. PMID: 14689577
  3. 인간 RAS 수퍼패밀리 단백질 및 관련 GTPase.  |  Colicelli, J. 2004. Sci STKE. 2004: RE13. PMID: 15367757
  4. 종양 진행 및 전이에서의 발암성 Ras.  |  Giehl, K. 2005. Biol Chem. 386: 193-205. PMID: 15843165
  5. Ras 원형질막 신호 전달 플랫폼.  |  Hancock, JF. and Parton, RG. 2005. Biochem J. 389: 1-11. PMID: 15954863
  6. 골지에서의 Ras 신호 전달.  |  Quatela, SE. and Philips, MR. 2006. Curr Opin Cell Biol. 18: 162-7. PMID: 16488589
  7. 구획화된 Ras/MAPK 신호.  |  Mor, A. and Philips, MR. 2006. Annu Rev Immunol. 24: 771-800. PMID: 16551266
  8. Ras, PI(3)K 및 mTOR 신호는 종양 세포 성장을 제어합니다.  |  Shaw, RJ. and Cantley, LC. 2006. Nature. 441: 424-30. PMID: 16724053
  9. 포유류 육종 레트로바이러스의 형질 전환 유전자와 관련된 인간 DNA의 세 가지 유전자.  |  Wong-Staal, F., et al. 1981. Science. 213: 226-8. PMID: 6264598

주문정보

제품명카탈로그 번호 단위가격수량관심품목

N-Ras 항체 (F155)

sc-31
100 µg/ml
$316.00

N-Ras (F155): m-IgG Fc BP-HRP 번들

sc-551590
100 µg Ab; 10 µg BP
$354.00

N-Ras (F155): m-IgG1 BP-HRP 번들

sc-551589
100 µg Ab; 20 µg BP
$354.00

N-Ras (F155): m-IgGκ BP-HRP 번들

sc-551591
100 µg Ab; 40 µg BP
$354.00

N-Ras 항체 (F155) AC

sc-31 AC
500 µg/ml, 25% agarose
$416.00

N-Ras 항체 (F155) HRP

sc-31 HRP
200 µg/ml
$316.00

N-Ras 항체 (F155) FITC

sc-31 FITC
200 µg/ml
$330.00

N-Ras 항체 (F155) PE

sc-31 PE
200 µg/ml
$343.00

N-Ras 항체 (F155) Alexa Fluor® 488

sc-31 AF488
200 µg/ml
$357.00

N-Ras 항체 (F155) Alexa Fluor® 546

sc-31 AF546
200 µg/ml
$357.00

N-Ras 항체 (F155) Alexa Fluor® 594

sc-31 AF594
200 µg/ml
$357.00

N-Ras 항체 (F155) Alexa Fluor® 647

sc-31 AF647
200 µg/ml
$357.00

N-Ras 항체 (F155) Alexa Fluor® 680

sc-31 AF680
200 µg/ml
$357.00

N-Ras 항체 (F155) Alexa Fluor® 790

sc-31 AF790
200 µg/ml
$357.00

Hi - I see a question from 7 years ago asking about the immunogen peptide sequence for this antibody with the reply being that it's unknown. Is that still the case, or if it's known now, could you share what the immunogen peptide sequence is? Thank you.

Asked by: Stephanie
Thank you for your question . The sequence is proprietary.
Answered by: BlakeJ
Date published: 2024-06-26

I have been use your NRAS antibody sc-31 , but i heard from my co-work said he tried to order this antibody but wait for a long time then he has been told it is discontinued. I want to order two more, let me know

Asked by: Hong Xiao
Thank you for your question. For accurate availability, shipping and pricing information regarding our antibodies, or to receive a quote, please contact Customer Service.
Answered by: Technical Service
Date published: 2024-03-28

Hi, can this N-Ras antibody(F155) be used to detect the Nras encoded by mutated NRAS, such as NrasQ61R?

Asked by: sssylvana
Thank you for your question. This antibody is raised against recombinant N-Ras p21 of human origin, recommended for detection of N-Ras p21 of mouse, rat and human origin by WB, IP, IF and IHC(P), we didn't test it on mutated NRAS.
Answered by: Technical Support 12
Date published: 2024-06-24

Hello, I am interested in "N-Ras Antibody (F155): sc-31" for Western blot use. What were the duration, buffers, Ab concentrations, and temperature/conditions (for blocking/probing) used to QC this Ab? Thank you in advance

Asked by: Anonymous
Thank you for your question. If you are using ECL for detection, we recommend blocking (PVDF membrane) in 5% nonfat milk + TBST for 1 hour at room temperature. We recommend using the same buffer (freshly prepared) as a diluent for both primary and secondary antibodies. If you are using Near-Infrared (NIR) Detection methods, then we recommend using our UltraCruz Blocking Reagent: sc-516214 instead of milk or BSA to reduce background. Recommended starting dilution for Western blot for the primary antibody is 1:200 (range 1:100-1:1,000). We recommend incubating primary antibody for 1 hour at room temperature, or overnight at 4C. Please refer to our recommended protocol online (or use the link below) for step-by-step detailed methods for how this antibody was tested for the data shown on our website. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2020-05-07

Your data sheet says" N-Ras (F155) is a mouse monoclonal antibody raised against recombinant p21 protein" Is it ok to tell what the immunogen peptide sequence is? Thanks

Asked by: Zhi123456789
Thank you for your question. The exact recombinant protein p21 immunogen sequence used to develop this mouse monoclonal antibody, N-Ras (F155): sc-31, is unknown.
Answered by: Technical Support
Date published: 2017-09-11

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: Anna99
No. Since no direct conjugates are available for this antibody, we would not recommend using it for IHC in mouse tissue. See pan Ras (C-4): sc-166691 for N-Ras antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647.
Answered by: Tech Service 5
Date published: 2016-12-16

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Anna99
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 5
Date published: 2016-12-16
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Rated 4 out of 5 by from N-Ras sc-31 for western blotVery good for western blotting (Hela cells). Detects a specific band at ~ 16 kDa.
Date published: 2024-02-10
Rated 4 out of 5 by from Application to ICCI have tested this Ab for the immunocytochemical detection of NRAS overexpressed in a thyroid-derived cell line. SIgnals were detected from cells transfected with the NRAS expression plasmid, although their intensity was low. No signals were observed in the non-transfected cell lines; therefore, detection of endogenous NRAS may be difficult.
Date published: 2023-02-21
Rated 4 out of 5 by from Specific for NRAS on WesternThis antibody specifically sees NRAS and does not crossreact with KRAS or HRAS. We use to confirm CRISPR of NRAS at 1:100. Leave primary on for 4 days for best results.
Date published: 2021-02-16
Rated 5 out of 5 by from NRASAplin lab used NRAS, SC-31 antibody for western blotting.
Date published: 2019-02-06
Rated 5 out of 5 by from Excellent AntibodyI tested this antibody on mouse brain lysate, detected a strong band (around 20 kDa) in WB (1:200 dilution).
Date published: 2018-07-09
Rated 5 out of 5 by from SC-31 NRASExcellent antibody. Specifically detects NRAS better than any other tested antibody. Can also be used in IF for overexpression systems (but not endogenous, although no RAS antibodies can be, this was the only one we tested that even worked specifically for overexpression).
Date published: 2017-10-11
Rated 5 out of 5 by from Great Western blot dataThe N-Ras Antibody (F155) shows strong Western blot signal with minimal background!
Date published: 2016-12-23
Rated 5 out of 5 by from Robust staining in paraffinRobust staining in paraffin-embedded human colon tissue but Unfortunately the nucleus have obvious coloring.
Date published: 2015-11-11
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N-Ras 항체 (F155) is rated 4.8 out of 5 by 13.
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