N-Myc 항체 (B8.4.B) WB와 IP으로 mouse, rat와 human origin의 N-Myc을 검출할것을 권장합니다.

N-Myc 항체 (B8.4.B): sc-53993

N-Myc 항체 (B8.4.B) is rated 4.7 out of 5 by 23.
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데이터 시트

    • 안티-N-Myc 항체 B8.4.B 는 마우스 monoclonal IgG2a κ N-Myc 항체, 166간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • raised against N-terminal recombinant N-Myc of human origin
    • 안티-N-Myc 항체 (B8.4.B)는 WB and IP으로 mouse, rat and human유래의 N-Myc 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-N-Myc 항체 (B8.4.B)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • N-Myc (B8.4.B): sc-53993무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for N-Myc Antibody (B8.4.B) for WB applications. These reagents are now offered in bundles with N-Myc Antibody (B8.4.B) (see ordering information below).
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can I use this antibody for ICC

Asked by: Jeew
Thank you for your question. The conjugated forms of this antibody, sc-53993, is warrantied for IF and IHC applications, but the unconjugated form is only warrantied for WB and IP use. We do have publications for IF/IHC, with this antibody, you can find them here: https://www.scbt.com/scbt/misc/citationResults.jsp?_requestid=476978 If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-03-22

What is the recommended concentration for use in IHC?

Asked by: Steev
Thank you for your question. We recommend using this mouse monoclonal antibody, N-Myc (B8.4.B): sc-53993, at a starting dilution of 1:50 (range of 1:50-1:500) for immunohistochemistry. A link to our recommended protocol is provided below as a reference. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-10-31

Does this cross react with c-myc?

Asked by: alexbk
Thank you for your question. No, this mouse monoclonal antibody, N-Myc (B8.4.B): sc-53993, is specific for N-Myc and does not cross-react with c-Myc.
Answered by: Technical Support
Date published: 2017-06-01

Would like to know the exact position of the recombinant protein used to raise this antibody. We need this info to interpret data on Cas9/Crisper gene depletion. Thanks

Asked by: Bisu
Thank you for your question. Unfortunately, the sequence of the recombinant protein used to generate this monoclonal antibody, N-Myc (B8.4.B): sc-53993, is not known. The only information describing the immunogen is that it was derived from the N-terminus of N-Myc of human origin. As an alternative, we do offer different mouse monoclonal antibodies against N-Myc where more detailed information is provided about the immunogen(s) used to develop these antibodies. For example, N-Myc (2): sc-142 with epitope mapping at residues 327-339 of N-Myc of human origin. Or N-Myc (C-4): sc-515099 specific for an epitope mapping between amino acids 443-464 within the C-terminus of N-Myc of human origin.
Answered by: Technical Support
Date published: 2017-01-16

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: AbPolly
Thank you for your inquiry. Instead of a standard anti-mouse secondary antibody, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service
Date published: 2016-12-20

Are there any issues in using this mouse monoclonal antibody to stain mouse tissues in IF or IHC?

Asked by: AbPolly
Thank you for your inquiry. This antibody can be used for staining mouse tissues. We recommend using a directly conjugated mouse monoclonal primary antibody. This N-Myc mouse monoclonal antibody is currently available conjugated to HRP, PE, FITC, AF488 and AF647.
Answered by: Technical Service
Date published: 2016-12-20
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Rated 5 out of 5 by from good anitbody MYCN b8.4.b recognizes a single band in D.rerio lysate at the predicted molecular weight.
Date published: 2021-04-20
Rated 5 out of 5 by from This antibody worked well for IF This antibody worked well for IF, using human neuroblastoma cell lines which have high endogenous N-Myc levels.
Date published: 2019-08-12
Rated 5 out of 5 by from Good antibody I tried a sample of this antibody and I found it works very well for WB. I made a 1:500 dilution for the detection of N-MYC in the SK-N-BE(2) cell line. I got a very strong signal and I project that I'm going to be able to work with bigger dilutions, such as 1:2000. The band appeared between 55 and 72 kDa, which conforms with the molecular weight of the protein, 67 kDa.
Date published: 2019-01-24
Rated 4 out of 5 by from Clean Bands, Probes Very Well Antibody produces clean, well-defined bands when used to detect N-Myc in human cancer cells, at a 1:1000 dilution.
Date published: 2018-01-04
Rated 2 out of 5 by from Not work No band detected in 293FT transfected with N-MYC plamid
Date published: 2017-09-15
Rated 5 out of 5 by from Very good antibody This N-Myc antibody works very well for chromatin immunoprecipitation.
Date published: 2017-09-13
Rated 4 out of 5 by from not suitable for turbot there is no band when i exposed for 25min ,dulited for 1:100
Date published: 2017-07-10
Rated 5 out of 5 by from Powerful Powerful, practical, effective, accurate and efficient!
Date published: 2017-07-05
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