myogenin 항체 (F5D) WB, IP, IF와 IHC(P)으로 mouse, rat와 human origin의 myogenin을 검출할것을 권장합니다.

myogenin 항체 (F5D): sc-12732

myogenin 항체 (F5D) is rated 4.8 out of 5 by 19.
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데이터 시트

    • myogenin 항체(F5D) 는 마우스 monoclonal IgG1κ, 384간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • epitope mapping within amino acids 138-158 of myogenin of rat origin
    • 안티-myogenin 항체 (F5D)는 WB, IP, IF and IHC(P)으로 mouse, rat and human유래의 myogenin 를 감지하는 데에 추천한다.
    • IP를 위해 agarose에 결합된 myogenin 항체 (F5D)를 제공한다.
    • IF, IHC(P) and FCM에 사용하는Alexa Fluor® 594 접합 제품도 제공한다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-12732 X, 200 µg/0.1 ml)이 있습니다.
    • See myogenin (5FD): sc-52903 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • myogenin (F5D): sc-12732무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for myogenin Antibody (F5D) for WB and IHC(P) applications. These reagents are now offered in bundles with myogenin Antibody (F5D) (see ordering information below).
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I need a secondary reagent to be used with myogenin Antibody (F5D): sc-12732 mouse monoclonal antibody by Western blot? I will be using the Licor system. Do you have a recommendation?

Asked by: jenni
We recommend using one our exclusive Mouse IgG Binding Proteins CFL 680 or CFL 790 as a secondary detection reagent. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Santa Cruz TS
Date published: 2016-12-15

Can myogenin Antibody (F5D): sc-12732 be used with my pig samples?

Asked by: jenni
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Santa Cruz TS
Date published: 2016-12-14
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Rated 5 out of 5 by from beautiful IF result This is good Antibody. Cell type: PMi28 (murine skeletal cells) Conc: 1μg/ml 4% PFA fix
Date published: 2019-08-26
Rated 5 out of 5 by from works fine we were given a free sample to try. It works just fine. It could detect as least as 5 ug of WCL loaded. And I re-used the diluted antibody for at least 5-8 times (kept the diluted AB at -20oC). For WB TBST was used, for blocking I used 5% milk in TBST.
Date published: 2018-08-27
Rated 4 out of 5 by from Good signal This antibody works well on human and mouse samples in WB
Date published: 2018-08-01
Rated 5 out of 5 by from Great product We used this antibody for western blot. It works for us.
Date published: 2017-09-27
Rated 4 out of 5 by from Great features! The antibody is work well in WB at 1:100,skeletal muscle cells.
Date published: 2017-09-01
Rated 5 out of 5 by from good result We have used it for WB and IF of 293 cells.WB 1:1000,IF 1:100,very good result ,sensitve and specific.
Date published: 2017-08-19
Rated 5 out of 5 by from great effective antibody I have been using this antibody (and a few others) from santa cruz for the past 3 years. I have always had good staining and clear bands. Furthermore, the price is quite competitive.
Date published: 2017-07-28
Rated 5 out of 5 by from Great antibody Great antibody for Western detection ,should be optimized for 1 hour primary incubation or 4℃ overnight,also the dilution should be optimized from 1:600 to 1:1000.
Date published: 2017-07-08
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