MyoD 항체 (G-1) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 MyoD을 검출할것을 권장합니다.

MyoD 항체 (G-1): sc-377460

MyoD 항체 (G-1) is rated 4.7 out of 5 by 19.
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데이터 시트

    • 안티-MyoD 항체 G-1 는 마우스 monoclonal IgG2b κ MyoD 항체, 95간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • mouse origin의 MyoD에서 내부에 위치한 1-318 아미노산을 항원으로 사용하였습니다.
    • 안티-MyoD 항체 (G-1)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 MyoD 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-MyoD 항체 (G-1)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-377460 X, 200 µg/0.1 ml)이 있습니다.
    • MyoD (G-1): sc-377460무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MyoD Antibody (G-1) for WB and IHC(P) applications. These reagents are now offered in bundles with MyoD Antibody (G-1) (see ordering information below).
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Is there a preferred way to fix C2C12 cells to use the Anti-MyoD Antibody (G-1) Alexa Fluor® 546?

Asked by: JEFZ
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-12-03

いつもお世話になります。 御社の下記の抗体を使用しています。 MyoD (G-1)  データシート等に掲載されているWBの予測分子量は約45kDaです。 しかしながら、この45kDaに加え、約70kDa近辺に強いバンドを検出しています。 我々の結果の場合、45kDaより70kDaのバンドの方が強く検出されます。 御社のホームページ上でも70kDa近辺に強いバンドが検出されているデータが掲載されています。 この70kDaのバンドの由来に関する情報をお持ちでしたら、ご教示ください。

Asked by: Shuji
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

您好,我想问一下,我买的myod的抗体,应该放在四度的,但是放在-20度冰箱24个小时了,请问会有影响吗?十分着急!麻烦你帮我解答一下谢谢!

Asked by: 诸葛钢铁
感谢您的提问。MyoD 抗体 (G-1): sc-377460 应该保存在4°C,不能冷冻。冷冻后对产品的质量可能会造成不良影响。 Thank you for your question. MyoD Antibody (G-1): sc-377460 should be stored at 4 °C. Do NOT FREEZE. The Quality of the product may be affected after freezing.
Answered by: Technical Support 12
Date published: 2021-07-07

I had used MyoD C-20 to detect MyoD in chicken myoblasts. It was great. However, C-20 was discontiuned and MyoD G-1 was produced instead. So, I brought the G-1, but the detection was not as good as C-20. Is there any way to get C-20?

Asked by: megimetel
Thank you for your question. Unfortunately, the rabbit polyclonal antibody, MyoD (C-20): sc-304, has been discontinued since 2016. It is no longer available for sale or distribution. We do not have any data or product citations yet using this mouse monoclonal antibody in chicken or avian samples. Please contact our Technical Service Department and we could assist you by performing sequence analysis to predict potential cross reactivity of our MyoD antibodies with chicken samples.
Answered by: Technical Support
Date published: 2020-03-26

HI, I'm interested in detecting MYOD in bovine cells . do you know if it cross rects? thanks

Asked by: Anonymous
Thank you for your question. This antibody hasn’t been tested in bovine samples. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined, so we can not perform sequence analysis at this time to predict potential cross reactivity with that species.
Answered by: Tech Service
Date published: 2019-06-28

Buenos días, estoy haciendo una inmunoprecipitacion con MyoD y quiero ver su anticuerpo HDAC1, pero la banda que me sale a 60 kDa no tengo claro si es HDAC1 o es la IgG, podrian decirme el peso al que me deberian salir las IgG??

Asked by: Anonymous
Muchas gracias por su pregunta. El peso molecular de la IgG es de 150 kDa, la cadena pesada de aprox 50 kDa mientras la ligera de aprox 25 kDa. Si está realizando otro método de detección, puede encontrar todos nuestros protocolos en: https://www.scbt.com/scbt/es/resources/protocols. Si necesita más información, no dude en contactar con nosotros: [email protected]
Answered by: Technical Support Europe
Date published: 2019-02-27

Can I use This antibody in the mouse skeletal muscles?

Asked by: sunny22
Thank you for your question. Yes, this antibody has been previously published for the detection of MyoD in mouse skeletal muscle. Please refer to the following publication for additional details: PMID: 28638997.
Answered by: Technical Support
Date published: 2019-01-25

The Western shown for this antibody shows a band at close to 55 kd. The western for MyoD 5.8A shows a band between 37 and 43 kd. Both are for MyoD. Please explain this discrepancy.

Asked by: SBSPhD
Thank you for your question. The base predicted weight for MyoD determined by sequence is approximately 35 kDa, however the protein appears to go through modification that results in the observed weight running at a variety of higher sizes. Publications have observed weights from 36-38 kDa through 43-45 kDa for the primary band, with some publications also reporting a band around 55-65 kDa. Due to this variability in size observed in various sample types, anything within this range that is clean and consistent is generally accepted. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Technical Service
Date published: 2017-02-21
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Rated 5 out of 5 by from Good antibody We purchased this antibody for immunostainning and western blot. It works very well for both the applications in cultured myoblasts.
Date published: 2018-05-06
Rated 4 out of 5 by from good ab I got a free sample from SCBT, and i tested it on primary satellite cell at 1/50. it worked well for Immunofluorescence.
Date published: 2017-10-13
Rated 5 out of 5 by from Great for pull down assays. Got this antibody for my immunoprecipitation project for human samples. Works great. Western blotting with MyoD western antibody shows clear double bands below 50KD IgG heavy chain band which is commonly detect in human samples. Decided to move on our work with this antibody.
Date published: 2017-09-29
Rated 5 out of 5 by from Great product We used this product for immunostaining in satellite cells. Works well for us.
Date published: 2017-09-27
Rated 5 out of 5 by from Great Antibody I received the free trial and found that this antibody was very specific for the primary myoblast cultures I am working with. Fixed cells with methanol at -20C for 5 minutes and obtained great images.
Date published: 2017-08-03
Rated 4 out of 5 by from Dear I bought it a mouth ago.I think it is very well.The concentration of MyoD is 1:1000.
Date published: 2017-07-05
Rated 4 out of 5 by from It is ok Used at 1:1000 in C2C12 cells. It is ok. There are also some non specific bands there.
Date published: 2017-06-02
Rated 5 out of 5 by from worked well MyoD antibody is suitable for western blot analysis in NIH/3T3 cell, and it worked well.
Date published: 2017-05-24
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