Date published: 2025-9-7

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MyoD 항체 (G-1): sc-377460

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데이터 시트
  • MyoD 항체 G-1 는 마우스 monoclonal IgG2b κ MyoD 항체, 144간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • mouse origin의 MyoD에서 내부에 위치한 1-318 아미노산을 항원으로 사용하였습니다.
  • MyoD 항체 (G-1)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 MyoD 를 감지하는 데에 추천한다.
  • 항-MyoD 항체(G-1)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-377460 X, 200 µg/0.1 ml)이 있습니다.
  • MyoD (G-1): sc-377460무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG2b BP-HRPm-IgGκ BP-HRP 는 MyoD 항체 (G-1) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은MyoD 항체(G-1) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다).

빠른 링크

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MyoD 항체(G-1)는 마우스, 쥐 및 인간 유래의 MyoD 단백질을 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgG2b κ 마우스 모노클로날 MyoD 항체(MYOD1 항체라고도 함)입니다. MyoD 항체(G-1)는 비접합 항-MyoD 항체 형태뿐만 아니라 아가로스, HRP, PE, FITC 및 다중 Alexa Fluor® 접합체를 포함한 여러 접합 형태의 항-MyoD 항체 형태로도 사용할 수 있습니다. 근세포의 분화는 긍정적으로 작용하는 여러 가지 인자와 부정적으로 작용하는 인자에 의해 조절됩니다. 근육 세포 발달 조절에 중요한 역할을 하는 것으로 알려진 헬릭스-루프-헬릭스(HLH) 단백질의 잘 특성화된 제품군에는 MyoD, 미오게닌, Myf-5 및 Myf-6(MRF-4 또는 헤룰린으로도 지정됨)이 있습니다. 흥미로운 점은 대부분의 근육 세포가 커밋된 상태에서는 MyoD 또는 Myf-5를 발현하지만 분화를 유도하면 모두 마이오제닌의 발현이 켜진다는 점입니다. MyoD 전사인자는 적어도 세 개의 서로 다른 유전자로 구성된 더 광범위하게 발현되는 bHLH 유전자 계열인 E 계열의 산물과 헤테로다이머를 형성합니다: E2A, IF2 및 HEB. MyoD-E 헤테로다이머는 많은 근육 특이 말단 분화 유전자의 상류 조절 서열에서 기능적으로 중요한 요소인 컨센서스(CANNTG) E 박스 표적 부위에 열렬히 결합합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

MyoD 항체 (G-1) 참고문헌:

  1. 골격근 아세포에서 세포 주기 조절 인자와 근 형성 인자 MyoD 사이의 누화.  |  Kitzmann, M. and Fernandez, A. 2001. Cell Mol Life Sci. 58: 571-9. PMID: 11361092
  2. [MyoD 조절에 대한 새로운 통찰력: 횡문근육종 경로에 관여?]  |  Tintignac, LA., et al. 2001. Bull Cancer. 88: 545-8. PMID: 11459700
  3. MyoD는 E-단백질 HEB베타와 시너지 효과를 발휘하여 근성 분화를 유도합니다.  |  Parker, MH., et al. 2006. Mol Cell Biol. 26: 5771-83. PMID: 16847330
  4. 탈신경화된 근육, 사용하지 않는 근육, 운동된 근육에서 MyoD의 역할.  |  Legerlotz, K. and Smith, HK. 2008. Muscle Nerve. 38: 1087-100. PMID: 18642380
  5. MyoD는 수모세포종의 종양 억제 유전자입니다.  |  Dey, J., et al. 2013. Cancer Res. 73: 6828-37. PMID: 24092238
  6. 히스톤 탈메틸화 효소 KDM4B는 MyoD와 상호작용하여 C2C12 근아세포에서 근성 분화를 조절합니다.  |  Choi, JH., et al. 2015. Biochem Biophys Res Commun. 456: 872-8. PMID: 25534856
  7. PRMT1은 R121에서 MyoD 아르기닌 메틸화를 통해 미오게닌 전사를 활성화합니다.  |  Liu, Q., et al. 2019. Biochim Biophys Acta Gene Regul Mech. 1862: 194442. PMID: 31634638
  8. FOXP1은 MyoD와 상호작용하여 전사 및 근아세포 전환을 억제합니다.  |  Wright, WE., et al. 2021. J Cell Signal. 2: 9-26. PMID: 33554216
  9. 근육 줄기세포 역학 및 이질성을 연구하기 위한 MyoD 녹인 리포터 마우스 라인 생성.  |  Fujita, R., et al. 2023. iScience. 26: 106592. PMID: 37250337
  10. MyoD 및 c-fos 발현.  |  Walsh, K. 1993. Nature. 365: 611-2. PMID: 8413625

주문정보

제품명카탈로그 번호 단위가격수량관심품목

MyoD 항체 (G-1)

sc-377460
200 µg/ml
$316.00

MyoD (G-1): m-IgGκ BP-HRP 번들

sc-523335
200 µg Ab, 40 µg BP
$354.00

MyoD (G-1): m-IgG2b BP-HRP 번들

sc-549109
200 µg Ab; 10 µg BP
$354.00

MyoD 항체 (G-1) X

sc-377460 X
200 µg/0.1 ml
$316.00

MyoD 항체 (G-1) AC

sc-377460 AC
500 µg/ml, 25% agarose
$416.00

MyoD 항체 (G-1) HRP

sc-377460 HRP
200 µg/ml
$316.00

MyoD 항체 (G-1) FITC

sc-377460 FITC
200 µg/ml
$330.00

MyoD 항체 (G-1) PE

sc-377460 PE
200 µg/ml
$343.00

MyoD 항체 (G-1) Alexa Fluor® 488

sc-377460 AF488
200 µg/ml
$357.00

MyoD 항체 (G-1) Alexa Fluor® 546

sc-377460 AF546
200 µg/ml
$357.00

MyoD 항체 (G-1) Alexa Fluor® 594

sc-377460 AF594
200 µg/ml
$357.00

MyoD 항체 (G-1) Alexa Fluor® 647

sc-377460 AF647
200 µg/ml
$357.00

MyoD 항체 (G-1) Alexa Fluor® 680

sc-377460 AF680
200 µg/ml
$357.00

MyoD 항체 (G-1) Alexa Fluor® 790

sc-377460 AF790
200 µg/ml
$357.00

I am using the MyoD antibody (G-1, sc-377460) and need to confirm the exact epitope it recognizes. My protein is a truncated MYOD1 (from Cys101 to C-terminus with an N-terminal His-tag). Could you clarify if this antibody binds to the C-terminal region?

Asked by: crazy emsa
Thank you for your question. MyoD Antibody (G-1): sc-377460 was raised against amino acids 1-318 of MyoD of mouse origin, representing full-length protein. No epitope mapping has been performed for this antibody as of yet, so its exact binding site is not known at this point in time.
Answered by: Tech Support Europe
Date published: 2025-07-01

Can sc-377460 recognize bovine MyoD in Western blot analysis and IHC?

Asked by: M Kobayashi
I tried this antibody (x1000) to detect recombinant bovine MyoD expressed in 293T cells and native MyoD in differentiating C2C12 cells by Western analysis. Approximately 45 kDa protein was clearly detected in 293T cells transfected with MyoD-expressing vector and differentiating C2C12 cells. However, a strong non-specific signal (~50 kDa) with some fainter bands were also detected in both cell line. Therefore, I would like to conclude that this antibody can detect bovine MyoD in the Western blot analysis. M Kobayashi
Answered by: M Kobayashi
Date published: 2025-04-24

Is there a preferred way to fix C2C12 cells to use the Anti-MyoD Antibody (G-1) Alexa Fluor® 546?

Asked by: JEFZ
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-12-03

いつもお世話になります。 御社の下記の抗体を使用しています。 MyoD (G-1)  データシート等に掲載されているWBの予測分子量は約45kDaです。 しかしながら、この45kDaに加え、約70kDa近辺に強いバンドを検出しています。 我々の結果の場合、45kDaより70kDaのバンドの方が強く検出されます。 御社のホームページ上でも70kDa近辺に強いバンドが検出されているデータが掲載されています。 この70kDaのバンドの由来に関する情報をお持ちでしたら、ご教示ください。

Asked by: Shuji
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

您好,我想问一下,我买的myod的抗体,应该放在四度的,但是放在-20度冰箱24个小时了,请问会有影响吗?十分着急!麻烦你帮我解答一下谢谢!

Asked by: 诸葛钢铁
感谢您的提问。MyoD 抗体 (G-1): sc-377460 应该保存在4°C,不能冷冻。冷冻后对产品的质量可能会造成不良影响。 Thank you for your question. MyoD Antibody (G-1): sc-377460 should be stored at 4 °C. Do NOT FREEZE. The Quality of the product may be affected after freezing.
Answered by: Technical Support 12
Date published: 2021-07-07

I had used MyoD C-20 to detect MyoD in chicken myoblasts. It was great. However, C-20 was discontiuned and MyoD G-1 was produced instead. So, I brought the G-1, but the detection was not as good as C-20. Is there any way to get C-20?

Asked by: megimetel
Thank you for your question. Unfortunately, the rabbit polyclonal antibody, MyoD (C-20): sc-304, has been discontinued since 2016. It is no longer available for sale or distribution. We do not have any data or product citations yet using this mouse monoclonal antibody in chicken or avian samples. Please contact our Technical Service Department and we could assist you by performing sequence analysis to predict potential cross reactivity of our MyoD antibodies with chicken samples.
Answered by: Technical Support
Date published: 2020-03-26

HI, I'm interested in detecting MYOD in bovine cells . do you know if it cross rects? thanks

Asked by: Anonymous
Thank you for your question. This antibody hasn’t been tested in bovine samples. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined, so we can not perform sequence analysis at this time to predict potential cross reactivity with that species.
Answered by: Tech Service
Date published: 2019-06-28

Buenos días, estoy haciendo una inmunoprecipitacion con MyoD y quiero ver su anticuerpo HDAC1, pero la banda que me sale a 60 kDa no tengo claro si es HDAC1 o es la IgG, podrian decirme el peso al que me deberian salir las IgG??

Asked by: Anonymous
Muchas gracias por su pregunta. El peso molecular de la IgG es de 150 kDa, la cadena pesada de aprox 50 kDa mientras la ligera de aprox 25 kDa. Si está realizando otro método de detección, puede encontrar todos nuestros protocolos en: https://www.scbt.com/scbt/es/resources/protocols. Si necesita más información, no dude en contactar con nosotros: spain@scbt.com
Answered by: Technical Support Europe
Date published: 2019-02-27
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Rated 1 out of 5 by from Nuclear staining in all cellsIn my hand this antibody stains all cells in the lymph node. No specificity at all. I've tried dilution in the range 1:50 to 1:1000 with different physical antigen retrival using different buffers.
Date published: 2023-01-23
Rated 5 out of 5 by from Good antibodyWe purchased this antibody for immunostainning and western blot. It works very well for both the applications in cultured myoblasts.
Date published: 2018-05-06
Rated 4 out of 5 by from good abI got a free sample from SCBT, and i tested it on primary satellite cell at 1/50. it worked well for Immunofluorescence.
Date published: 2017-10-13
Rated 5 out of 5 by from Great for pull down assays.Got this antibody for my immunoprecipitation project for human samples. Works great. Western blotting with MyoD western antibody shows clear double bands below 50KD IgG heavy chain band which is commonly detect in human samples. Decided to move on our work with this antibody.
Date published: 2017-09-29
Rated 5 out of 5 by from Great productWe used this product for immunostaining in satellite cells. Works well for us.
Date published: 2017-09-27
Rated 5 out of 5 by from Great AntibodyI received the free trial and found that this antibody was very specific for the primary myoblast cultures I am working with. Fixed cells with methanol at -20C for 5 minutes and obtained great images.
Date published: 2017-08-03
Rated 4 out of 5 by from DearI bought it a mouth ago.I think it is very well.The concentration of MyoD is 1:1000.
Date published: 2017-07-05
Rated 4 out of 5 by from It is okUsed at 1:1000 in C2C12 cells. It is ok. There are also some non specific bands there.
Date published: 2017-06-02
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MyoD 항체 (G-1) is rated 4.6 out of 5 by 20.
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