Thank you for your question. The base predicted weight for MyoD determined by sequence is approximately 35 kDa, however the protein appears to go through modification that results in the observed weight running at a variety of higher sizes. Publications have observed weights from 36-38 kDa through 43-45 kDa for the primary band, with some publications also reporting a band around 55-65 kDa. Due to this variability in size observed in various sample types, anything within this range that is clean and consistent is generally accepted. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]
>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Date published: 2017-02-21