Date published: 2025-9-9

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Mucin 2/MUC2 항체 (Ccp58): sc-7314

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데이터 시트
  • Mucin 2/MUC2 항체(Ccp58) 는 마우스 monoclonal IgG1κ, 87간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human의 Mucin 2 내부 영역에 있는 아미노산 에 매핑되는 합성 펩티드에 대해 제기됨
  • 안티-Mucin 2/MUC2 항체 (Ccp58)는 IF and IHC(P)으로 mouse, rat and human유래의 Mucin 2 를 감지하는 데에 추천한다.
  • 또한 Alexa Fluor® 488 (sc-7314 AF488) 또는 Alexa Fluor® 594 (sc-7314 AF594)와 결합된 200 µg/ml로 IF, IHC(P) 및 FCM용으로도 이용 가능
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • Mucin 2/MUC2 (Ccp58): sc-7314무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP 는 Mucin 2/MUC2 항체 (Ccp58) for IHC(P) applications. 이 시약은 이제 Mucin 2/MUC2 항체 (Ccp58)와 함께 번들로 제공됩니다(아래 주문 정보 참조).

빠른 링크

더보기

뮤신 2/MUC2 항체(Ccp58)는 IF 및 IHC(P)에 의해 마우스, 랫 및 인간 기원의 뮤신 2를 검출하는 IgG1 κ 마우스 단클론 뮤신 2 항체이다. 뮤신 2/MUC2 항체(Ccp58)는 결합 및 비결합 항-뮤신 2 항체 형태 모두로 이용 가능하다. 뮤신은 60개의 염기쌍(Mucin 1), 69개의 염기쌍(Mucin 2) 및 51개의 염기쌍(Mucin 3)에 의해 암호화된 가변 수의 직렬 반복(VNTR)으로 구성된 기본 구조를 갖는 고도로 글리코실화된 분비 단백질 계열이다. 반복 횟수는 고도로 다형성이며 상이한 대립유전자들 사이에서 다양하다. 뮤신 1 단백질은 분비된 형태 외에 유형 I 막 단백질로 발현된다. 뮤신 1은 유방암을 포함한 상피 종양에서 특이하게 발현된다. 뮤신 2는 장 및 기도의 상피를 코팅하고 결장 종양과 관련이 있다. 뮤신 3은 다양한 점액 겔의 주요 공성분이며 정상 세포 및 종양 세포에서 광범위하게 발현된다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

Mucin 2/MUC2 항체 (Ccp58) 참고문헌:

  1. N- 연결된 올리고당은 장 점액 Muc2의 이황화물 의존적 이량체화에 중요한 역할을 합니다.  |  Bell, SL., et al. 2003. Biochem J. 373: 893-900. PMID: 12744721
  2. 인간 MUC2 장 점액에는 중앙 반복 영역의 상류와 하류에 시스테인이 풍부한 하위 도메인이 있습니다.  |  Gum, JR., et al. 1992. J Biol Chem. 267: 21375-83. PMID: 1400449
  3. 인간 MUC2 뮤신 유전자는 위장관 암종 세포주에서 Cdx 호모도메인 단백질에 의해 전사적으로 조절됩니다.  |  Mesquita, P., et al. 2003. J Biol Chem. 278: 51549-56. PMID: 14525978
  4. 쥐의 Muc2 뮤신 유전자는 장 세포에서 아연 손가락 GATA-4 전사인자에 의해 전사적으로 조절됩니다.  |  van der Sluis, M., et al. 2004. Biochem Biophys Res Commun. 325: 952-60. PMID: 15541382
  5. 새로운 인간 장 뮤신 유전자에서 유래한 cDNA의 분자 복제.  |  Gum, JR., et al. 1990. Biochem Biophys Res Commun. 171: 407-15. PMID: 2393399
  6. 인간 췌장 종양 뮤신 cDNA의 복제 및 시퀀싱.  |  Lan, MS., et al. 1990. J Biol Chem. 265: 15294-9. PMID: 2394722
  7. 인간 DF3 유방암 관련 항원을 코딩하는 cDNA의 분리 및 시퀀싱.  |  Siddiqui, J., et al. 1988. Proc Natl Acad Sci U S A. 85: 2320-3. PMID: 2895474
  8. 뮤신-2 녹아웃은 장 상피의 세포 간 접합 결함, 미토콘드리아 손상 및 ATP 고갈의 모델입니다.  |  Borisova, MA., et al. 2020. Sci Rep. 10: 21135. PMID: 33273633
  9. 뮤신 2의 결실은 마우스에서 수반되는 대사 이상을 동반한 대장염을 유도합니다.  |  Ye, J., et al. 2021. Am J Physiol Gastrointest Liver Physiol. 320: G791-G803. PMID: 33728986
  10. MUC2 및 관련 박테리아 인자: 궤양성 대장염의 치료 표적.  |  Yao, D., et al. 2021. EBioMedicine. 74: 103751. PMID: 34902790
  11. 궤양성 대장염의 뮤신 발현 프로파일: 조직학적 점막 치유에 대한 새로운 통찰력.  |  Leoncini, G., et al. 2024. Int J Mol Sci. 25: PMID: 38339134
  12. DF3/MUC1 유방암 항원과 Grb2 및 Sos/Ras 교환 단백질의 연관성.  |  Pandey, P., et al. 1995. Cancer Res. 55: 4000-3. PMID: 7664271

주문정보

제품명카탈로그 번호 단위가격수량관심품목

Mucin 2/MUC2 항체 (Ccp58)

sc-7314
200 µg/ml
$316.00

Mucin 2/MUC2 (Ccp58): m-IgG Fc BP-HRP 번들

sc-526497
200 µg Ab; 10 µg BP
$354.00

Mucin 2/MUC2 (Ccp58): m-IgG1 BP-HRP 번들

sc-531870
200 µg Ab; 20 µg BP
$354.00

Mucin 2/MUC2 항체 (Ccp58) Alexa Fluor® 488

sc-7314 AF488
200 µg/ml
$357.00

Mucin 2/MUC2 항체 (Ccp58) Alexa Fluor® 546

sc-7314 AF546
200 µg/ml
$357.00

Mucin 2/MUC2 항체 (Ccp58) Alexa Fluor® 594

sc-7314 AF594
200 µg/ml
$357.00

Mucin 2/MUC2 항체 (Ccp58) Alexa Fluor® 680

sc-7314 AF680
200 µg/ml
$357.00

Mucin 2/MUC2 항체 (Ccp58) Alexa Fluor® 790

sc-7314 AF790
200 µg/ml
$357.00

Does this MUC2 antibody have cross-reactivity with MUC1 ?

Asked by: jm123
Thank you for your question. The antibody has been known to only detect MUC2. https://www.uniprot.org/uniprotkb/Q02817/entry#sequences
Answered by: BlakeJ
Date published: 2024-04-15

i received sc-7314 at room temperature though it says to store at 4C. Will antibody integrity be compromised? also- do you have a CoA for this item? Thank you.

Asked by: jujuB
Some of our antibodies ship out ambient. They can last up to 5 days out of storage. We do not have CoAs for our antibodies.
Answered by: Technical Service
Date published: 2023-12-18

I tried using this antibody for IF at a 1:50 and 1:100 dilution in 5% BSA to stain for MUC-2 secreted by HT29-MTX cells but it did not work well.  Is there an antibody that is better suited for this application using HT29-MTX cells?

Asked by: isramire
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext. 1, allowing for a more interactive discussion of this and other related questions.”
Answered by: Blake J
Date published: 2021-11-17

The Rabbit polyclonal muc2 primary antibody H-300 Santa Cruz technologies was been discontinued. Is there an alternative MUC2 antibody that can be used to stain mouse muc2 for IF. What would be the Alexa488 secondary antibody to use?

Asked by: Anonymous
Yes, this antibody sc-7314 should be suitable for IF on mouse tissues. Please contact technical support for further assistance.
Answered by: Tech Service 1
Date published: 2020-11-16

What concentration do you recommend for this antibody for mouse detection via IF and IHC?

Asked by: crrivet
Thank you for your question. The recommended starting dilution is 1:50 (range 1:50-1:500).
Answered by: Technical Support
Date published: 2019-09-27

Will this antibody work in a Sandwich Elisa?

Asked by: KathrinR
Thank you for your question. We have not tested this antibody in an ELISA and so this is not a recommended application at this time.
Answered by: Technical Support
Date published: 2019-04-26

This might be a novice question, however, shouldn't a primary antibody never be from the same host as the target species? Nonetheless, you suggest using this mouse monoclonal antibody to detect mucin 2 in mice. Could you elaborate how this works?

Asked by: Mwolter
Thank you for your question. There is no problem with using a mouse monoclonal antibody for detection of the corresponding protein in mouse cells or tissues. The main concern would be that your secondary antibody (anti-mouse IgG) may cross react non-specifically with endogenous mouse IgG expressed in your samples. This is only really an issue if your samples are rich in endogenous IgG. In that case, we would recommend optimizing your secondary antibody, blocking and incubation conditions. Alternatively, you can always use a directly conjugated primary antibody and avoid using any secondary antibody for detection.
Answered by: Technical Support
Date published: 2019-04-13

Will this antibody cross-react in my zebrafish samples?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 4
Date published: 2017-01-13
  • y_2025, m_8, d_27, h_5CST
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Rated 3 out of 5 by from It works well in WBWe tested this Ab by WB at 1:1000 dilution. Strong band and no background. We'll also test it in IF on Human Intestinal Colonoids.
Date published: 2023-03-22
Rated 1 out of 5 by from do not work on IHCdo not work on IHC-DAB and IHC-IF in mouse small intestine and large intestine. dilution 1:50.
Date published: 2020-08-17
Rated 1 out of 5 by from Does not stain goblet cells in murine intestine IFWe purchased two lots of this antibody and they do not stain for Mucin2, instead they appear to stain extracellular matrix or some other protein in the basal lamina propria (attached). We believe this is lot specific (attached), however customer service has chosen to ignore this after we politely brought it to their attention. We do not recommend purchasing this antibody, and are disappointed but not surprised by santa cruz's response to our concerns. We are however amused by the staff 5-star ratings of this antibody, fair play.
Date published: 2019-11-14
Rated 4 out of 5 by from 使用方便,功能齐全我在一个月前购买了mucin-2 此产品,并用于黏液层免疫组化染色,实验效果显著,这个产品使用方便,且抗体效价很高。
Date published: 2019-06-08
Rated 1 out of 5 by from No effect on mouse colon IF stainingNo effect on colonic IF staining in mice, concentration 1:100
Date published: 2019-05-11
Rated 1 out of 5 by from Mouse small intestine tissueDoesn't work for mouse small intestine tissue via IF on cryosections & IF on mouse small intestinal organoids.
Date published: 2018-07-08
Rated 5 out of 5 by from strong bandNo non-specific binding and background ,just strong band,I am not recommend use Tween-20 when blocking the membrane,it may cause background.
Date published: 2017-07-01
Rated 5 out of 5 by from specific stainingWe use Mucin 2 antibody by Immunohistochemistry of human tonsil tissue stained with Anti-Mucin 2 antibody , we got the specific staining.
Date published: 2017-05-28
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Mucin 2/MUC2 항체 (Ccp58) is rated 3.8 out of 5 by 16.
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