LAT Antibody (B-3): sc-373706

LAT Antibody (B-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects LAT protein in mouse, rat, and human samples by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). LAT antibody (B-3) is available in non-conjugated form and various conjugates, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® options. LAT, or linker for activation of T cells, is an integral membrane protein crucial for T cell receptor signaling and immune response initiation. Upon T cell receptor activation, LAT associates with key signaling molecules such as phospholipase C gamma 1, GRB2, and p85 subunit of phosphoinositide 3-kinase, leading to phosphorylation by ZAP-70/Syk tyrosine kinases. This phosphorylation activates transcription factors like AP-1 and NFAT, mediating T cell proliferation and differentiation. LAT undergoes palmitoylation, a post-translational modification essential for proper localization to lipid rafts within the cell membrane, enhancing signaling capabilities. LAT′s function as a linker protein in T cell activation highlights LAT antibody (B-3)′s value for researchers studying T cell signaling pathways.

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LAT Antibody (B-3) References:

1. Linker for activation of T cells (LAT), a novel immunohistochemical marker for T cells, NK cells, mast cells, and megakaryocytes: evaluation in normal and pathological conditions.  |  Facchetti, F., et al. 1999. Am J Pathol. 154: 1037-46. PMID: 10233842
2. Modulation of the electrophoretic mobility of the linker for activation of T cells (LAT) by the calcineurin inhibitors CsA and FK506: LAT is a potential substrate for PKC and calcineurin signaling pathways.  |  Cho, CS., et al. 2003. Cell Signal. 15: 85-93. PMID: 12401523
3. Structural basis for differential recognition of tyrosine-phosphorylated sites in the linker for activation of T cells (LAT) by the adaptor Gads.  |  Cho, S., et al. 2004. EMBO J. 23: 1441-51. PMID: 15029250
4. Inhibition of T-cell receptor signal transduction and viral expression by the linker for activation of T cells-interacting p12(I) protein of human T-cell leukemia/lymphoma virus type 1.  |  Fukumoto, R., et al. 2007. J Virol. 81: 9088-99. PMID: 17582004
5. Analysis of the linker for activation of T cells and the linker for activation of B cells in natural killer cells reveals a novel signaling cassette, dual usage in ITAM signaling, and influence on development of the Ly49 repertoire.  |  Whittaker, GC., et al. 2008. Blood. 112: 2869-77. PMID: 18645037
6. Vitamin E reverses impaired linker for activation of T cells activation in T cells from aged C57BL/6 mice.  |  Marko, MG., et al. 2009. J Nutr. 139: 1192-7. PMID: 19403707
7. Palmitoylation-dependent plasma membrane transport but lipid raft-independent signaling by linker for activation of T cells.  |  Hundt, M., et al. 2009. J Immunol. 183: 1685-94. PMID: 19592663
8. The linker for activation of B cells (LAB)/non-T cell activation linker (NTAL) regulates triggering receptor expressed on myeloid cells (TREM)-2 signaling and macrophage inflammatory responses independently of the linker for activation of T cells.  |  Whittaker, GC., et al. 2010. J Biol Chem. 285: 2976-85. PMID: 19948717
9. Beta-catenin inhibits T cell activation by selective interference with linker for activation of T cells-phospholipase C-γ1 phosphorylation.  |  Driessens, G., et al. 2011. J Immunol. 186: 784-90. PMID: 21149602
10. The Us3 Protein of Herpes Simplex Virus 1 Inhibits T Cell Signaling by Confining Linker for Activation of T Cells (LAT) Activation via TRAF6 Protein.  |  Yang, Y., et al. 2015. J Biol Chem. 290: 15670-15678. PMID: 25907557

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-LAT Antibody (B-3) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-LAT Antibody (B-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

LAT Antibody (B-3) Specifications

Alternate Product Name: LAT

Product Clone Name: B-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-233

Epitope Target: LAT

Epitope Species: human

Product Reactivity/Detection: LAT

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for LAT Antibody (B-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred LAT antibody is LAT Antibody (11B.12): sc-53550.