Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LAP2 CRISPR/Cas9 KO Plasmid (h): sc-403613
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LAP2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LAP2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LAP2 Antibody (3A3): sc-81610
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LAP2 CRISPR/Cas9 KO Plasmid (h) | sc-403613 | 20 µg | $397.00 |
Overview
TMPO encodes lamina-associated polypeptide 2 (LAP2), an inner nuclear membrane protein that binds lamins and chromatin to organize the nuclear lamina and maintain nuclear envelope architecture. LAP2 isoforms contribute to tethering peripheral heterochromatin, regulating DNA replication timing, and coordinating post-mitotic nuclear reassembly, linking TMPO to cell-cycle control and genome stability pathways. Through interactions with lamin A/C and other envelope components, LAP2 influences mechanotransduction and nuclear integrity under proliferative or stress conditions. Altered TMPO/LAP2 expression or nuclear envelope dysfunction is associated with aberrant nuclear morphology observed in cancer and other disorders involving compromised genome organization.
LAP2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TMPO gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TMPO together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TMPO open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LAP2 protein expression.
This CRISPR knockout system enables efficient generation of TMPO-deficient cell models for investigation of LAP2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TMPO exon(s) critical for LAP2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TMPO genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LAP2 CRISPR/Cas9 KO Plasmid (h) and LAP2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TMPO locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LAP2 HDR Plasmid (h) and LAP2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TMPO homology arms to support homology-directed repair at defined TMPO target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.