L-Caldesmon Antibody (F-10): sc-25339

L-Caldesmon Antibody (F-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects L-Caldesmon of human origin and Caldesmon of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). L-Caldesmon (F-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Caldesmon plays a crucial role in muscle contraction and cellular motility by binding to actin filaments, which is essential for maintaining the structural integrity and functionality of muscle tissues. Caldesmon exists in two main forms: muscular Caldesmon, predominantly found in smooth muscle, and non-muscular Caldesmon, which is widely expressed in various non-muscle tissues. Multiple isoforms, resulting from alternative splicing of the Caldesmon gene, allow for a diverse range of regulatory functions in different cellular contexts. Muscular Caldesmon (isoform 1), also known as high molecular weight Caldesmon, is primarily located on thin filaments in smooth muscle, while non-muscular isoforms (2-5) are involved in various cellular processes across different tissues. Caldesmon′s interaction with other actin-binding proteins, such as Filamin 1, Nebulin, and Villin, further underscores its importance in the regulation of the cytoskeleton and muscle function, making anti-L-Caldesmon antibody (F-10) a valuable tool for researchers studying muscle biology and related disorders.

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L-Caldesmon Antibody (F-10) References:

1. Developmental regulation of villin gene expression in the epithelial cell lineages of mouse digestive and urogenital tracts.  |  Maunoury, R., et al. 1992. Development. 115: 717-28. PMID: 1425351
2. Gene delivery of l-caldesmon protects cytoskeletal cell membrane integrity against adenovirus infection independently of myosin ATPase and actin assembly.  |  Haxhinasto, K., et al. 2004. Am J Physiol Cell Physiol. 287: C1125-38. PMID: 15189814
3. Caldesmon binds to smooth muscle myosin and myosin rod and crosslinks thick filaments to actin filaments.  |  Marston, S., et al. 1992. J Muscle Res Cell Motil. 13: 206-18. PMID: 1534566
4. Phosphorylated l-caldesmon is involved in disassembly of actin stress fibers and postmitotic spreading.  |  Kordowska, J., et al. 2006. Exp Cell Res. 312: 95-110. PMID: 16289153
5. Caldesmon is an integral component of podosomes in smooth muscle cells.  |  Eves, R., et al. 2006. J Cell Sci. 119: 1691-702. PMID: 16595550
6. Role of TGF-beta1-independent changes in protein neosynthesis, p38alphaMAPK, and cdc42 in hydrogen peroxide-induced senescence-like morphogenesis.  |  Chrétien, A., et al. 2008. Free Radic Biol Med. 44: 1732-51. PMID: 18325348
7. Effect of l-caldesmon on osteoclastogenesis in RANKL-induced RAW264.7 cells.  |  Liou, YM., et al. 2018. J Cell Physiol. 233: 6888-6901. PMID: 29377122
8. L-caldesmon alters cell spreading and adhesion force in RANKL-induced osteoclasts.  |  Chan, CL., et al. 2019. J Biomed Sci. 26: 12. PMID: 30678675
9. Actin-binding and dimerization domains of HeLa cell filamin.  |  Weihing, RR. 1988. Biochemistry. 27: 1865-9. PMID: 3378035
10. The complete primary structure of human nebulin and its correlation to muscle structure.  |  Labeit, S. and Kolmerer, B. 1995. J Mol Biol. 248: 308-15. PMID: 7739042
11. Multiple-sited interaction of caldesmon with Ca(2+)-calmodulin.  |  Huber, PA., et al. 1996. Biochem J. 316 (Pt 2): 413-20. PMID: 8687382
12. cDNA cloning of mouse nebulin. Evidence that the nebulin-coding sequence is highly conserved among vertebrates.  |  Zhang, JQ., et al. 1996. Eur J Biochem. 239: 835-41. PMID: 8774733

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-L-Caldesmon Antibody (F-10) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-L-Caldesmon Antibody (F-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

L-Caldesmon Antibody (F-10) Specifications

Alternate Product Name: L-Caldesmon

Product Clone Name: F-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 494-793

Epitope Target: L-caldesmon

Epitope Species: human

Product Reactivity/Detection: L-Caldesmon of human origin and Caldesmon of mouse and rat origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for L-Caldesmon Antibody (F-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred H-Caldesmon antibody is H-Caldesmon Antibody (h-CALD): sc-58703.