ISG15 항체 (F-9) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 ISG15을 검출할것을 권장합니다.

ISG15 항체 (F-9): sc-166755

ISG15 항체 (F-9) is rated 4.5 out of 5 by 24.
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데이터 시트

    • 안티-ISG15 항체 F-9 는 마우스 monoclonal IgG1 κ ISG15 항체, 57간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • raised against amino acids 1-150 mapping at the N-terminus of ISG15 of human origin
    • 안티-ISG15 항체 (F-9)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 ISG15 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-ISG15 항체 (F-9)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • ISG15 (F-9): sc-166755무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ISG15 Antibody (F-9) for WB and IHC(P) applications. These reagents are now offered in bundles with ISG15 Antibody (F-9) (see ordering information below).
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-The datasheet specifies this is "a mouse monoclonal antibody raised against amino acids 1-150" but ISG15 is 165 aa. Is there more information on the epitope (not highlighted in the protein sequence tab)? -5% milk in TBS 0.1% Tween 4 C overnighincubation?

Asked by: autumnos
Thank you for your question. Yes, this information is correct, the antibody is raised against a recombinant protein corresponding to amino acids 1-150 of ISG15 of human origin. The exact epitope recognized by this monoclonal antibody has not been determined. We do recommend blocking in 5% nonfat milk in TBST (0.05% Tween-20) for one hour at room temperature, and incubating the primary antibody at the starting dilution of 1:200 in 5% nonfat milk + TBST for one hour at room temperature, or overnight at 4C.
Answered by: Technical Support
Date published: 2019-07-03

What is the light chain of ISG15 Antibody (F-9): sc-166755 ?

Asked by: cjMara
ISG15 Antibody (F-9) is a mouse monoclonal IgG1 with kappa light chain.
Answered by: Technical Support 9
Date published: 2017-09-13
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Rated 4 out of 5 by from Very good ISG15 antibody It works really well, single band, strong. No background. Samples are liver homogenates from bacteria-infected mice.
Date published: 2021-06-04
Rated 4 out of 5 by from Works in Western Clean and sharp band observed by Western blotting at expected height; worked well in lysates of various cell lines; as expected, higher expression upon IFN stimulation
Date published: 2020-09-28
Rated 5 out of 5 by from The antibody is good I got a free test antibody, the antibody is very good, it also can be used for IP.
Date published: 2019-02-26
Rated 5 out of 5 by from Strong signal F-9 antibody produces a strong signal on WB at 1:1000 dilution. The band observed is a bit "lighter" than expected but that's probably caused by using Tris-Glycine system.
Date published: 2018-02-02
Rated 5 out of 5 by from sc-166755 This antibody works great for WB (cell lysates; mouse)
Date published: 2018-01-02
Rated 5 out of 5 by from Nice IHC staining I used it for IHC of human cells an gives an intense and specific signal.
Date published: 2017-12-22
Rated 5 out of 5 by from Works good in WB This Ab with 1:1000 dilution was used for the detection of human samples and the interested band could be observed clearly in WB image.
Date published: 2017-09-28
Rated 5 out of 5 by from an excellent antibody for WB This antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
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