IL-6R alpha 항체 (D-8) WB, IP, IF와 ELISA으로 mouse와 rat origin의 IL-6Rα을 검출할것을 권장합니다.

IL-6Rα 항체 (D-8): sc-374259

IL-6Rα 항체 (D-8) is rated 5.0 out of 5 by 5.
  • y_2022, m_11, d_28, h_21
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.34
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_5
  • loc_ko_KR, sid_374259, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 112ms
  • REVIEWS, PRODUCT
loading
5
5
4
0
3
0
2
0
1
0

데이터 시트

    • IL-6R alpha 항체 D-8 는 마우스 monoclonal IgG1 κ IL-6Rα 항체, 14간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • 항원결정부는 mouse origin의 IL-6Rα의 내부에 위치한 433-460의 아미노산에 해당됩니다.
    • IL-6R alpha 항체 (D-8)는 WB, IP, IF and ELISA으로 mouse and rat유래의 IL-6Rα 를 감지하는 데에 추천한다.
    • Anti-IL-6R alpha Antibody (D-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • IL-6Rα (D-8): sc-374259무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for IL-6Rα Antibody (D-8) for WB applications. These reagents are now offered in bundles with IL-6Rα Antibody (D-8) (see ordering information below).
loading

Does this antibody stain for soluble IL-6R? Thanks

Asked by: Kim12
Thank you for your question. IL-6Rα Antibody (D-8): sc-374259 is specific for an epitope mapping between amino acids 433-460 at the C-terminus of IL-6Rα of mouse origin. This part of the protein is present in both the membrane-bound and soluble isoforms of IL-6Rα. Please contact our technical service if you require further information.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-21

is this antibody suitable for IHC or IF from paraffin-embedded sections?

Asked by: jack2
Thank you for your question. We recommend using this antibody, IL-6Rα (D-8): sc-374259, for IF, or immunofluorescent staining on fresh frozen tissues or cells fixed in acetone or methanol. The antibody is not recommended for IHC(P) on formalin-fixed paraffin-embedded tissue sections. We recommend using a starting dilution of 1:50 (range of 1:50-1:500) for IF or IHC. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2020-07-01

What is your recommended western blotting secondary antibody for use with IL-6Rα (D-8): sc-374259?

Asked by: jenniferc
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 4
Date published: 2017-02-04
  • y_2022, m_11, d_28, h_21CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.34
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_ko_KR, sid_374259, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 107ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Very efficient antibody indeed. Antibody use many times, bought two antibodies but different batches this time, the quality is stable, the product is good.
Date published: 2017-04-28
Rated 5 out of 5 by from nice staining. Very pleased with IHC data; clear, specific, very nice staining.
Date published: 2017-04-19
Rated 5 out of 5 by from Specific for mouse and rat The (D-8) clone is a great choice for specific detection of mouse and rat IL-6R alpha. However, if you are looking for a human-reactive antibody we recommend an alternative clone, (H-7): sc-373708
Date published: 2017-01-19
Rated 5 out of 5 by from Produced positive Western Blot data of IL Produced positive Western Blot data of IL-6R expression in WEHI-3 and CTLL-2 whole cell lysates. -SCBT QC
Date published: 2015-01-19
Rated 5 out of 5 by from Nice Western Blot data of IL Nice Western Blot data of IL-6R expression in M1 whole cell lysate. -SCBT QC
Date published: 2013-12-14
  • y_2022, m_11, d_28, h_21
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.34
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_5
  • loc_ko_KR, sid_374259, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 16ms
  • REVIEWS, PRODUCT