Thank you for your question. We recommend using this antibody, IL-6Rα (D-8): sc-374259, for IF, or immunofluorescent staining on fresh frozen tissues or cells fixed in acetone or methanol. The antibody is not recommended for IHC(P) on formalin-fixed paraffin-embedded tissue sections. We recommend using a starting dilution of 1:50 (range of 1:50-1:500) for IF or IHC. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]
>, or by live chat directly on our website, www.scbt.com.
Date published: 2020-07-01