HuR/ELAV1 Antibody (3A2): sc-5261

HuR Antibody (3A2) is a mouse monoclonal IgG1 kappa light chain antibody that detects HuR protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). HuR (3A2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. HuR protein, also known as ELAV-like RNA binding protein 1, plays a crucial role in post-transcriptional regulation of gene expression by binding to AU-rich elements in the 3′-untranslated region of target mRNAs. This interaction stabilizes mRNA transcripts that would otherwise be rapidly degraded, thereby influencing the expression levels of genes involved in critical cellular processes such as growth, differentiation, and response to stress. HuR protein is ubiquitously expressed and is often overexpressed in various tumors, highlighting importance in cancer biology. HuR contains three RNA recognition motifs, which are essential for binding activity, and shuttling between the nucleus and cytoplasm is regulated by a nuclear export signal, allowing modulation of mRNA stability and translation in response to cellular signals. This dynamic regulation underscores HuR significance in maintaining cellular homeostasis and potential as a therapeutic target in cancer and other diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

HuR/ELAV1 Antibody (3A2) References:

1. Cytoplasmic localization is required for the mammalian ELAV-like protein HuD to induce neuronal differentiation.  |  Kasashima, K., et al. 1999. Genes Cells. 4: 667-83. PMID: 10620013
2. The RNA-binding protein HuR in human cancer: A friend or foe?  |  Wu, X. and Xu, L. 2022. Adv Drug Deliv Rev. 184: 114179. PMID: 35248670
3. Relationship between HuR and tumor drug resistance.  |  Ma, Q., et al. 2023. Clin Transl Oncol. 25: 1999-2014. PMID: 36947360
4. Targeting the RNA-Binding Protein HuR in Cancer.  |  Finan, JM., et al. 2023. Cancer Res. 83: 3507-3516. PMID: 37683260
5. A TRIM21-based bioPROTAC highlights the therapeutic benefit of HuR degradation.  |  Fletcher, A., et al. 2023. Nat Commun. 14: 7093. PMID: 37925433
6. Differential activity of ELAV-like RNA-binding proteins in human neuroblastoma.  |  Chagnovich, D., et al. 1996. J Biol Chem. 271: 33587-91. PMID: 8969226
7. The Elav-like proteins bind to a conserved regulatory element in the 3'-untranslated region of GAP-43 mRNA.  |  Chung, S., et al. 1997. J Biol Chem. 272: 6593-8. PMID: 9045688
8. Identification of HuR as a protein implicated in AUUUA-mediated mRNA decay.  |  Myer, VE., et al. 1997. EMBO J. 16: 2130-9. PMID: 9155038
9. Differential expression of the neuroendocrine genes Hel-N1 and HuD in small-cell lung carcinoma: evidence for down-regulation of HuD in the variant phenotype.  |  King, PH. 1997. Int J Cancer. 74: 378-82. PMID: 9291425
10. Sequential expression and role of Hu RNA-binding proteins during neurogenesis.  |  Wakamatsu, Y. and Weston, JA. 1997. Development. 124: 3449-60. PMID: 9310339
11. RNA stabilization by the AU-rich element binding protein, HuR, an ELAV protein.  |  Peng, SS., et al. 1998. EMBO J. 17: 3461-70. PMID: 9628881
12. Neuron-specific hel-N1 and HuD as novel molecular markers of neuroblastoma: a correlation of HuD messenger RNA levels with favorable prognostic features.  |  Ball, NS. and King, PH. 1997. Clin Cancer Res. 3: 1859-65. PMID: 9815574

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HuR/ELAV1 Antibody (3A2) has 526 citations in a variety of scientific publications.

Primary antibodies like Anti-HuR/ELAV1 Antibody (3A2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HuR/ELAV1 Antibody (3A2) Specifications

Alternate Product Name: ELAVL4

Product Clone Name: 3A2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: HuR

Epitope Species: human

Product Reactivity/Detection: HuR, HuB, HuC and HuD

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: Xenopus

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 526 citations in various scientific publications

Available Conjugates/Forms for HuR/ELAV1 Antibody (3A2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin