Date published: 2025-9-7

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HuR/ELAV1 항체 (3A2): sc-5261

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데이터 시트
  • HuR/ELAV1 항체 3A2 는 마우스 monoclonal IgG1 κ HuR/ELAV1 항체, 526간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human의 전체 길이 재조합 HuR에 대해 제기됨
  • HuR/ELAV1 항체 (3A2)는 WB, IP, IF, IHC(P) and ELISA으로mouse, rat, human and Xenopus유래의 HuR, HuB, HuC and HuD 를 감지하는 데에 추천한다
  • 항-HuR/ELAV1 항체(3A2)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • HuR/ELAV1 (3A2): sc-5261무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 는 HuR/ELAV1 항체 (3A2) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은HuR/ELAV1 항체(3A2) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다).

빠른 링크

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HuR 항체(3A2)는 마우스, 쥐, 인간 유래의 HuR 단백질을 웨스턴 블롯팅(WB), 면역침전법(IP), 면역형광법(IF), 면역조직화학법(IHC), 효소결합면역흡착분석법(ELISA)을 통해 검출하는 마우스 단일클론 IgG1 카파 경쇄 항체입니다. HuR(3A2) 항체는 비접합 형태와 아가로스, 과산화효소, 피코에리트린, 플루오레세인 이소티오시아네이트, 다중 알렉사 플루오르® 접합체를 포함한 다양한 접합 형태로 제공됩니다. ELAV-like RNA binding protein 1로도 알려진 HuR 단백질은 표적 mRNA의 3' 비번역 영역에 있는 AU-rich 요소에 결합함으로써 유전자 발현의 전사 후 조절에 중요한 역할을 합니다. 이 상호작용은 그렇지 않으면 빠르게 분해될 수 있는 mRNA 전사를 안정화시켜, 성장, 분화, 스트레스에 대한 반응과 같은 중요한 세포 과정에 관여하는 유전자의 발현 수준에 영향을 미칩니다. HuR 단백질은 어디에나 존재하며 다양한 종양에서 과발현되는 경우가 많기 때문에 암 생물학에서 그 중요성이 강조되고 있습니다. HuR은 결합 활동에 필수적인 세 가지 RNA 인식 모티프를 포함하고 있으며, 핵과 세포질 사이의 왕복은 핵 수출 신호에 의해 조절되어 세포 신호에 반응하여 mRNA 안정성과 번역의 조절이 가능합니다. 이러한 동적 조절은 세포 항상성 유지에 있어 HuR의 중요성과 암 및 기타 질병의 치료 표적으로서의 잠재력을 강조합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

HuR/ELAV1 항체 (3A2) 참고문헌:

  1. 포유류의 ELAV 유사 단백질 HuD가 신경세포 분화를 유도하려면 세포질 국소화가 필요합니다.  |  Kasashima, K., et al. 1999. Genes Cells. 4: 667-83. PMID: 10620013
  2. 인간 암의 RNA 결합 단백질 HuR: 친구인가 적인가?  |  Wu, X. and Xu, L. 2022. Adv Drug Deliv Rev. 184: 114179. PMID: 35248670
  3. HuR과 종양 약물 내성의 관계.  |  Ma, Q., et al. 2023. Clin Transl Oncol. 25: 1999-2014. PMID: 36947360
  4. 암에서 RNA 결합 단백질 HuR 표적화.  |  Finan, JM., et al. 2023. Cancer Res. 83: 3507-3516. PMID: 37683260
  5. TRIM21 기반 바이오프로탁은 HuR 분해의 치료적 이점을 강조합니다.  |  Fletcher, A., et al. 2023. Nat Commun. 14: 7093. PMID: 37925433
  6. 인간 신경모세포종에서 ELAV 유사 RNA 결합 단백질의 차별적 활성.  |  Chagnovich, D., et al. 1996. J Biol Chem. 271: 33587-91. PMID: 8969226
  7. 엘라브 유사 단백질은 GAP-43 mRNA의 3'-미번역 영역에 있는 보존된 조절 요소에 결합합니다.  |  Chung, S., et al. 1997. J Biol Chem. 272: 6593-8. PMID: 9045688
  8. AUUUA 매개 mRNA 붕괴에 관여하는 단백질로서 HuR의 동정.  |  Myer, VE., et al. 1997. EMBO J. 16: 2130-9. PMID: 9155038
  9. 소세포 폐암종에서 신경내분비 유전자 Hel-N1과 HuD의 차별적 발현: 변이 표현형에서 HuD의 하향 조절 증거.  |  King, PH. 1997. Int J Cancer. 74: 378-82. PMID: 9291425
  10. 신경 발생 중 Hu RNA 결합 단백질의 순차적 발현과 역할.  |  Wakamatsu, Y. and Weston, JA. 1997. Development. 124: 3449-60. PMID: 9310339
  11. AU가 풍부한 요소 결합 단백질인 ELAV 단백질인 HuR에 의한 RNA 안정화.  |  Peng, SS., et al. 1998. EMBO J. 17: 3461-70. PMID: 9628881
  12. 신경모세포종의 새로운 분자 마커로서의 뉴런 특이적 hel-N1 및 HuD: HuD 메신저 RNA 수준과 유리한 예후 특징의 상관관계.  |  Ball, NS. and King, PH. 1997. Clin Cancer Res. 3: 1859-65. PMID: 9815574

주문정보

제품명카탈로그 번호 단위가격수량관심품목

HuR/ELAV1 항체 (3A2)

sc-5261
200 µg/ml
$316.00

HuR/ELAV1 (3A2): m-IgG Fc BP-HRP 번들

sc-528135
200 µg Ab; 10 µg BP
$354.00

HuR/ELAV1 (3A2): m-IgGκ BP-HRP 번들

sc-520439
200 µg Ab, 40 µg BP
$354.00

HuR/ELAV1 (3A2): m-IgG1 BP-HRP 번들

sc-542781
200 µg Ab; 20 µg BP
$354.00

HuR/ELAV1 항체 (3A2) AC

sc-5261 AC
500 µg/ml, 25% agarose
$416.00

HuR/ELAV1 항체 (3A2) HRP

sc-5261 HRP
200 µg/ml
$316.00

HuR/ELAV1 항체 (3A2) FITC

sc-5261 FITC
200 µg/ml
$330.00

HuR/ELAV1 항체 (3A2) PE

sc-5261 PE
200 µg/ml
$343.00

HuR/ELAV1 항체 (3A2) Alexa Fluor® 488

sc-5261 AF488
200 µg/ml
$357.00

HuR/ELAV1 항체 (3A2) Alexa Fluor® 546

sc-5261 AF546
200 µg/ml
$357.00

HuR/ELAV1 항체 (3A2) Alexa Fluor® 594

sc-5261 AF594
200 µg/ml
$357.00

HuR/ELAV1 항체 (3A2) Alexa Fluor® 647

sc-5261 AF647
200 µg/ml
$357.00

HuR/ELAV1 항체 (3A2) Alexa Fluor® 680

sc-5261 AF680
200 µg/ml
$357.00

HuR/ELAV1 항체 (3A2) Alexa Fluor® 790

sc-5261 AF790
200 µg/ml
$357.00

Have you identified the exact epitope the Hur (3A2) antibody binds to now?

Asked by: SarahYC
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-02-09

HELLO,I want to do RIP and WB experiment in mouse,which antibody should I choose?HuR Antibody (3A2) or HuR Antibody (3A2) AC.?

Asked by: ncestt
Thank you for your question. If you need to use the same antibody for both Western blotting and immunoprecipitation, then we would recommend purchasing the unconjugated primary antibody, sc-5261: HuR (3A2). The ‘AC’ represents the primary antibody directly conjugated to agarose beads, and is only suitable for immunoprecipitation experiments.
Answered by: Technical Support
Date published: 2019-07-13

Is this antibody works well for detecting use flow cytometry?

Asked by: Yongxia
The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-01-17

I HAVE A QUESTION ABOUT THIS ANTIBODY. IT IS POSSIBLE TO REACT POSITIVELY AGAINST GST-FUSION PROTEINS?.

Asked by: FLOPITZOTSOA
Thank you for your question. This monoclonal antibody, HuR (3A2): sc-5261, is recommended for detection of HuR, HuB, HuC and HuD of mouse, rat, human and xenopus origin. The antibody is not recommended for detection of GST. However, the antibody is suitable for detection of GST-HuR, HuB, HuC or HuD fusion proteins.
Answered by: Technical Support
Date published: 2017-11-15

Hello, for research only I need to know whether this antibody (HuR (3A2): sc-5261) binds C-terminal or N-terminal HuR?

Asked by: Theresa Liebe
Thank you for your question. HuR Antibody (3A2): sc-5261 is raised against full length recombinant HuR of human origin, and we have not determined the exact epitope recognized by this mouse monoclonal antibody. According to the literature (PMID: 10737787), “the 3A2 epitope is located in RRM 1 (RNA Recognition Motif 1) of HuR, which is highly conserved among all ELAV proteins [82% identity between HuR, HuD, and Hel-N1/N2, and 72% identity between HuR and HuC].”
Answered by: Technical Support
Date published: 2017-06-08

Does this antibody work well with CLIP?

Asked by: beachbum
Thank you for your question. We have not tested this antibody for CLIP, and are not aware of any publications citing use of the antibody for that application. HuR Antibody (3A2): sc-5261 is a mouse monoclonal that has been cited in over 25 publications for immunoprecipitation (IP). Based on the antibody's performance in that application and the similarity with CLIP, it may also be suitable for that application, but that would have to be determined experimentally.
Answered by: Technical Support
Date published: 2017-03-10

What is the epitope of HuR Antibody (3A2): sc-5261?

Asked by: Dr Ninau Qelp
HuR Antibody (3A2): sc-5261 was raised against full length HuR of human origin. We have not mapped the epitope.
Answered by: Tech Service 2
Date published: 2016-12-15

Can HuR Antibody (3A2): sc-5261 be used for bovine samples?

Asked by: Dr Ninau Qelp
Yes. We have several publications that cite using HuR Antibody (3A2): sc-5261 in bovine samples. Please see PMID: 18206386, 20018854, and 22879735
Answered by: Tech Service 2
Date published: 2016-12-15
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Rated 5 out of 5 by from Detection of HuR that contains L-AzidohomoalanineAntibody sc-5261 was used successfully to detect HuR that was synthesized in the presence of L-Azidohomoalanine (AHA), an analog of L-methionine. Using click-chemistry, AHA-conaining HuR was biotinylated, affinity-purified with magnetic streptavidin-beads, and subjected to Western blot analysis.
Date published: 2025-08-16
Rated 5 out of 5 by from Good antibodyThe antibody worked perfectly as predicted. Recommended.
Date published: 2024-12-04
Rated 5 out of 5 by from 效果很好一直用santa的HuR货号为 SC-5261的抗体,无论是WB一抗,还是免疫共沉淀又或者是免疫荧光实验,效果都很好。值得购买。
Date published: 2024-06-11
Rated 5 out of 5 by from HuRdling SenescenceImmunohistochemical staining was performed using an anti-HuR antibody (sc-5261, Santa Cruz, TX, USA; 1:50). Western blotting was performed using the anti-HuR antibody (1:4000). IF (1:500) IH (1:50)
Date published: 2021-03-24
Rated 5 out of 5 by from 效果非常好我们一直在用这只抗体,可以做蛋白免疫印迹,蛋白免疫共沉淀,免疫荧光,特异性很高,效果非常好用,物有所值
Date published: 2020-08-17
Rated 1 out of 5 by from This review is for C23 Antibody (MS-3).C23 Antibody (MS-3) (Lot #H0818), which I purchased Nov. 2018, is not detecting any signal in BT20 breast and Hey ovarian cancer cells. I did IB multiple times with no success.
Date published: 2019-02-06
Rated 4 out of 5 by from review of sc-5261The anti-HuR 3A2 works very well in immunofluorescence
Date published: 2018-07-27
Rated 5 out of 5 by from HuR Antibody (3A2)HuR Antibody (3A2) works good in western blot from Cytosol fractions.
Date published: 2018-03-08
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HuR/ELAV1 항체 (3A2) is rated 4.7 out of 5 by 28.
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