hnRNP F 항체 (3H4) WB, IP, IF 와ELISA으로 human origin의 hnRNP F을 검출할것을 권장합니다.

hnRNP F 항체 (3H4): sc-32309

hnRNP F 항체 (3H4) is rated 4.3 out of 5 by 4.
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데이터 시트

    • 안티-hnRNP F 항체 3H4 는 마우스 monoclonal IgG1 κ hnRNP F 항체, 3간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • raised against full length recombinant hnRNPF of human origin
    • hnRNP F 항체 (3H4)는 WB, IP, IF and ELISA으로 human유래의 hnRNP F 를 감지하는 데에 추천한다 .
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-hnRNP F 항체 (3H4)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • hnRNP F (3H4): sc-32309무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.

What is the recommended fixation method for immunofluorescence staining with hnRNP F (3H4): sc-32309 monoclonal antibody?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Nice nuclear IF staining of HeLa cells We used this antibody for immunoflourescence staining of hnRNP-F. We were able to get nice signal intensity and images.
Date published: 2017-02-07
Rated 4 out of 5 by from Good specificity for WB in human hnRNP F Good specificity for WB in human hnRNP F transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-04-09
Rated 4 out of 5 by from Produced great immunofluorescence nuclear Produced great immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-15
Rated 4 out of 5 by from Good band in WB in K Good band in WB in K-562 nuclear extract. -SCBT QC
Date published: 2015-02-07
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