Date published: 2025-9-7
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GR/NR3C1/Glucocorticoid Receptor Antibody (G-5) - Western Blotting - Image 391357
GR 항체(G-5) Alexa Fluor® 488: sc-393232 AF488. A-431(A), HeLa(C) 및 NIH/3T3(D) 전체 세포 용해물 및 A-431 핵 추출물(B)에서 GR 발현의 직접 형광 웨스턴 블롯 분석. UltraCruz® 차단 시약으로 차단: sc-516214.
GR 항체(G-5) Alexa Fluor® 488: sc-393232 AF488. A-431(A), HeLa(C) 및 NIH/3T3(D) 전체 세포 용해물 및 A-431 핵 추출물(B)에서 GR 발현의 직접 형광 웨스턴 블롯 분석. UltraCruz® 차단 시약으로 차단: sc-516214.

GR/NR3C1/Glucocorticoid Receptor 항체 (G-5): sc-393232

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데이터 시트
  • GR/NR3C1/Glucocorticoid Receptor 항체 G-5 는 마우스 monoclonal IgG2b κ GR/NR3C1/Glucocorticoid Receptor 항체, 98간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human origin의 GR에서 내부에 위치한 121-420 아미노산을 항원으로 사용하였습니다.
  • GR/NR3C1/Glucocorticoid Receptor 항체 (G-5)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 GR α and GR β 를 감지하는 데에 추천한다.
  • 항-GR/NR3C1/Glucocorticoid Receptor 항체(G-5)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-393232 X, 200 µg/0.1 ml)이 있습니다.
  • GR/NR3C1/Glucocorticoid Receptor (G-5): sc-393232의 무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP 와 m-IgG2b BP-HRP 는 GR/NR3C1/Glucocorticoid Receptor 항체 (G-5) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은GR/NR3C1/Glucocorticoid Receptor 항체(G-5) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다).

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GR 항체(G-5)는 마우스, 쥐 및 인간 기원의 GR 단백질을 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgG2b κ 마우스 단일 클론 GR 항체(글루코코르티코이드 수용체 항체 또는 NR3C1 항체로도 지정됨)입니다. GR 항체(G-5)는 비접합 항-GR 항체 형태뿐만 아니라 아가로스, HRP, PE, FITC 및 여러 Alexa Fluor® 접합체를 포함한 여러 접합 형태의 항-GR 항체 형태로도 사용할 수 있습니다. 글루코코르티코이드 수용체(GR)는 글루코코르티코이드의 효과를 매개하는 유비쿼터스적으로 발현되는 전사인자입니다. 가장 풍부한 이소폼은 GR α입니다. GR은 글루코코르티코이드에 반응하여 유전자의 발현을 유도하거나 억제하여 세포 사멸, 세포 성장 및 분화와 같은 과정을 매개합니다. GR에 의해 억제되는 중요한 종류의 유전자는 전사인자 AP-1에 의해 제어됩니다. GR은 또한 글루코코르티코이드에 의한 유전자 발현 유도를 제한하는 요인으로 밝혀졌습니다. GR은 HSP 90과 복합체를 형성하여 비리간드 결합 수용체를 전사적으로 비활성화하는 것으로 밝혀졌습니다. 더 중요한 것은 신호 도메인이 결여된 돌연변이 GR은 구성적으로 활성 상태를 유지한다는 것입니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

GR/NR3C1/Glucocorticoid Receptor 항체 (G-5)

sc-393232
200 µg/ml
$316.00

GR/NR3C1/Glucocorticoid Receptor (G-5): m-IgG Fc BP-HRP 번들

sc-526231
200 µg Ab; 10 µg BP
$354.00

GR/NR3C1/Glucocorticoid Receptor (G-5): m-IgG2b BP-HRP 번들

sc-549698
200 µg Ab; 10 µg BP
$354.00

GR/NR3C1/Glucocorticoid Receptor 항체 (G-5) X

sc-393232 X
200 µg/0.1 ml
$316.00

GR/NR3C1/Glucocorticoid Receptor 항체 (G-5) AC

sc-393232 AC
500 µg/ml, 25% agarose
$416.00

GR/NR3C1/Glucocorticoid Receptor 항체 (G-5) HRP

sc-393232 HRP
200 µg/ml
$316.00

GR/NR3C1/Glucocorticoid Receptor 항체 (G-5) FITC

sc-393232 FITC
200 µg/ml
$330.00

GR/NR3C1/Glucocorticoid Receptor 항체 (G-5) PE

sc-393232 PE
200 µg/ml
$343.00

GR/NR3C1/Glucocorticoid Receptor 항체 (G-5) Alexa Fluor® 488

sc-393232 AF488
200 µg/ml
$357.00

GR/NR3C1/Glucocorticoid Receptor 항체 (G-5) Alexa Fluor® 546

sc-393232 AF546
200 µg/ml
$357.00

GR/NR3C1/Glucocorticoid Receptor 항체 (G-5) Alexa Fluor® 594

sc-393232 AF594
200 µg/ml
$357.00

GR/NR3C1/Glucocorticoid Receptor 항체 (G-5) Alexa Fluor® 647

sc-393232 AF647
200 µg/ml
$357.00

GR/NR3C1/Glucocorticoid Receptor 항체 (G-5) Alexa Fluor® 680

sc-393232 AF680
200 µg/ml
$357.00

GR/NR3C1/Glucocorticoid Receptor 항체 (G-5) Alexa Fluor® 790

sc-393232 AF790
200 µg/ml
$357.00

RRID?

Asked by: lyh15
Thank you for your question. RRID:AB_2687823
Answered by: Ling Chen
Date published: 2024-12-25

I have delta GR in which we have only the c-terminus of GR from 506 aa to the 794 aa so which antibody would be suitable for this deleted version of GR?

Asked by: KIRTIRAVAT
Thank you for contacting Santa Cruz Biotechnology. For assistance please contact technical service by phone or email. Please call (800)-457-3801 or email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2024-10-17

is this antibody applicable to the IHC/IP/WB plant model system?

Asked by: KIRTIRAVAT
Thank you for your question. GR/NR3C1/Glucocorticoid Receptor Antibody (G-5): sc-393232 is recommended for detection of GR α and GR β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA.
Answered by: Tech Support Europe
Date published: 2024-10-15

Setting: Western Blot 20 mcg protein of tissue lysate (mouse tissue); antibody diluted 1:1000 in 5%BSA. Problem: I have a good band at 95 kDa , but also a clear (unexpected) band around 50kDa. What could this be?

Asked by: Arnot
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Does GR antibody (G-5): sc-393232 work in ChIP assay for rat muscle?

Asked by: jymrobin
Thank you for your question. This antibody has been published previously for ChIP applications (PMID: 30994266 and PMID: 30698747). This antibody is reactive with mouse, rat and human. We do not have any data specifically in rat muscle.
Answered by: Technical Support
Date published: 2019-07-18

How well does the antibody work with rat brain tissue for western blotting, and what would be the optimum concentration?

Asked by: nico315
Thank you for your question. We have not yet tested this antibody in rat brain tissue. We do have data in mouse brain tissue extract. The primary antibody was used at the starting dilution of 1:200, and loading 50ug tissue extract.
Answered by: Technical Support
Date published: 2019-07-13

Is the GR ANTIBODY (G-5) X sc-393232X the same as the GR ANTIBODY (G-5) sc-393232 but just at a higher concentration?

Asked by: MegCon
Thank you for your question. Yes, sc-393232 X is provided at a 10x higher concentration. It is provided as 200µg in 0.1ml while the standard product is provided as 200µg in 1ml.
Answered by: Tech Service
Date published: 2019-01-14

Hi, please let me know what concentration of the  GR Antibody (G-5): sc-393232 would be optimal for  Flow Cytometry.  Thank you.

Asked by: nacharya18
Thank you for your question. We have not yet tested this mouse monoclonal, GR Antibody (G-5): sc-393232, for Flow Cytometry so this application is not recommended at this time. We do offer this antibody directly conjugated to a variety of fluorochromes, including: phycoerythrin (sc-393232 PE), fluorescein (sc-393232 FITC), Alexa Fluor® 488 (sc-393232 AF488) or Alexa Fluor® 647 (sc-393232 AF647), 200 µg/ml, for IF, IHC(P) and FCM. If you purchase one of the direct conjugates, we can extend our product warranty to cover the application of Flow Cytometry. A link to our recommended protocol for FCM is below. We recommend using approximately 1 µg of fluorochrome-conjugated antibody to 100 µl of the prepared cell suspension (equal to 1 million cells) to each tube. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support
Date published: 2017-03-03
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Rated 5 out of 5 by from Works well in mouse brain at 1:100!We bought this antibody last month. We ran a dilution series in mouse brain on free floating 50-micron sections for the following primary antibody dilution amounts: 1:50, 1:100, and 1:200 (+ a no primary control). There was minimal difference between these concentrations and no staining present in the no primary control so we plan to proceed by using it at 1:100.
Date published: 2021-12-14
Rated 4 out of 5 by from Good for WB of mouse hippocampusWe ordered the sample and proved it for WB of mice hippocampal tissue. Gave a nice specific band for the GR as assessed in WT and Emx1-Nr3c1 KO mice.
Date published: 2021-08-03
Rated 4 out of 5 by from Glucocorticoid receptor antibodyGood staining of mice hypothalamus. Sample: mouse cortex fixed with 4% PFA for 16hr at 4℃, followed by sucrose infiltration and OCT embedding. Staining: floatation staining for 48hr at 4℃ (1:300 or 1:500, 300 was better), followed by 2nd antibody staining. FYI, I have not tried shorter staining such as 1hr at RT or overnight at 4℃.
Date published: 2021-07-28
Rated 3 out of 5 by from Glucocorticoid receptor antibodyThis antibody works better in nuclear then in cytoplasmic fraction of liver (dilution 1:250).
Date published: 2021-02-17
Rated 3 out of 5 by from Glucocorticoid receptor antibodyThis antibody works better in nuclear then in cytoplasmic fraction of liver (dilution 1:250).
Date published: 2021-02-17
Rated 5 out of 5 by from Works excelent in westernblotGR was detected in mesenchymal stem cells and used at a dilution of 1;250 1 H at room temp
Date published: 2020-09-18
Rated 5 out of 5 by from PERFECT CHOICEThis antibody works well. Used 1:500 in tbs 0,1%tween to detect GR in different cell line extract (45gs of total proteins; MCF-7 breast cancer cells and KP2 and SUIT2 pancreatic cancer cells), detect only one specific band at 95 kDa. I’ll buy this antibody.
Date published: 2019-09-27
Rated 5 out of 5 by from Works well!It works well for ChIP on both mouse hepatocytes and mouse livers.
Date published: 2019-04-26
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GR/NR3C1/Glucocorticoid Receptor 항체 (G-5) is rated 4.1 out of 5 by 28.
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