GR 항체 (G-5) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 GR α and GR β을 검출할것을 권장합니다.

GR 항체 (G-5): sc-393232

GR 항체 (G-5) is rated 4.1 out of 5 by 23.
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Datasheets

    • 안티-GR 항체 G-5 는 마우스 monoclonal IgG2b (kappa light chain) GR 항체 이며 200 µg/ml으로 제공합니다.
    • human origin의 GR에서 내부에 위치한 121-420 아미노산을 항원으로 사용하였습니다.
    • 안티-GR 항체 (G-5)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 GR α and GR β 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-GR 항체 (G-5)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-393232 X, 200 µg/0.1 ml)이 있습니다.
    • 강력추천하는 conventional secondary anti-mouse IgG reagents의 대체제품 보기m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP).
    • GR (G-5): sc-393232무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.

Does GR antibody (G-5): sc-393232 work in ChIP assay for rat muscle?

Asked by: jymrobin
Thank you for your question. This antibody has been published previously for ChIP applications (PMID: 30994266 and PMID: 30698747). This antibody is reactive with mouse, rat and human. We do not have any data specifically in rat muscle.
Answered by: Technical Support
Date published: 2019-07-18

How well does the antibody work with rat brain tissue for western blotting, and what would be the optimum concentration?

Asked by: nico315
Thank you for your question. We have not yet tested this antibody in rat brain tissue. We do have data in mouse brain tissue extract. The primary antibody was used at the starting dilution of 1:200, and loading 50ug tissue extract.
Answered by: Technical Support
Date published: 2019-07-13

Is the GR ANTIBODY (G-5) X sc-393232X the same as the GR ANTIBODY (G-5) sc-393232 but just at a higher concentration?

Asked by: MegCon
Thank you for your question. Yes, sc-393232 X is provided at a 10x higher concentration. It is provided as 200µg in 0.1ml while the standard product is provided as 200µg in 1ml.
Answered by: Tech Service
Date published: 2019-01-14

Hi, please let me know what concentration of the  GR Antibody (G-5): sc-393232 would be optimal for  Flow Cytometry.  Thank you.

Asked by: nacharya18
Thank you for your question. We have not yet tested this mouse monoclonal, GR Antibody (G-5): sc-393232, for Flow Cytometry so this application is not recommended at this time. We do offer this antibody directly conjugated to a variety of fluorochromes, including: phycoerythrin (sc-393232 PE), fluorescein (sc-393232 FITC), Alexa Fluor® 488 (sc-393232 AF488) or Alexa Fluor® 647 (sc-393232 AF647), 200 µg/ml, for IF, IHC(P) and FCM. If you purchase one of the direct conjugates, we can extend our product warranty to cover the application of Flow Cytometry. A link to our recommended protocol for FCM is below. We recommend using approximately 1 µg of fluorochrome-conjugated antibody to 100 µl of the prepared cell suspension (equal to 1 million cells) to each tube. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support
Date published: 2017-03-03

Hi, I am a graduate student working in a Xenopus Laevis laboratory, I would like to test this antibody in our system, is it possible to get an aliquot of it to test it? 

Asked by: Northwestern University
Thank you for your question. We do not know if this mouse monoclonal, GR Antibody (G-5): sc-393232, will work for Xenopus Laevis. For a limited time, we are currently offering free 10 µg samples to test of the antibody. Please contact our Technical Service Department to request a sample. Please call us toll-free at (800) 457-3801 option 2, e-mail [email protected], or use our live chat!
Answered by: Technical Support
Date published: 2017-02-24

I need to use an antibody to detect the protein in samples of avian origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Service 11
Date published: 2017-01-11

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-11
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Rated 5 out of 5 by from Works excelent in westernblot GR was detected in mesenchymal stem cells and used at a dilution of 1;250 1 H at room temp
Date published: 2020-09-18
Rated 5 out of 5 by from PERFECT CHOICE This antibody works well. Used 1:500 in tbs 0,1%tween to detect GR in different cell line extract (45gs of total proteins; MCF-7 breast cancer cells and KP2 and SUIT2 pancreatic cancer cells), detect only one specific band at 95 kDa. I’ll buy this antibody.
Date published: 2019-09-27
Rated 5 out of 5 by from Works well! It works well for ChIP on both mouse hepatocytes and mouse livers.
Date published: 2019-04-26
Rated 5 out of 5 by from Works Beautifully I've got a sample of this AB and used to perform IF in mouse brain tissue. I've obtained a nice staining as using a 1:1000 dilution. I've ordered the product already
Date published: 2018-04-23
Rated 5 out of 5 by from Nice antibody I bought this month ago and worked extremely well with western blot in breast cancer cell lines
Date published: 2017-11-22
Rated 1 out of 5 by from GR Antibody (G-5) I tried the staining with this antibody but did not work.
Date published: 2017-10-26
Rated 5 out of 5 by from Excellent antibody This antibody is excellent for IF, IHC and western blotting.
Date published: 2017-10-20
Rated 5 out of 5 by from VERY GOOD The antibody was applied in IHC analysis in HeLa cells in the presence or absence of DEX. Dilution 1:50
Date published: 2017-10-20
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