GM-CSF Antibody (B6-2-hGMCSF): sc-32753

GM-CSF Antibody (B6-2-hGMCSF) is a mouse monoclonal IgG1 kappa light chain antibody that detects GM-CSF protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-GM-CSF antibody (B6-2-hGMCSF) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a crucial pleiotropic cytokine that plays a significant role in immune response by stimulating proliferation and differentiation of hematopoietic progenitor cells into granulocytes and macrophages. The mature form of human GM-CSF consists of 127 amino acids, while the murine counterpart has 124 amino acids and shares approximately 60% homology with the human version. GM-CSF is a glycoprotein that promotes growth of immune cells and enhances functional capabilities, such as phosphorylation of cytosolic phospholipase A2 (cPLA2) in human neutrophils, which is essential for production of inflammatory mediators. Phosphorylation of cPLA2 leads to an increase in enzymatic activity, thereby amplifying inflammatory response. GM-CSF serves as a vital target for research in immunology and therapeutic interventions, particularly in conditions where immune system is compromised or dysregulated.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

GM-CSF Antibody (B6-2-hGMCSF) References:

1. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.  |  Abrams, JS., et al. 1992. Immunol Rev. 127: 5-24. PMID: 1387110
2. The clinical use of colony stimulating factors.  |  Moore, MA. 1991. Annu Rev Immunol. 9: 159-91. PMID: 1910675
3. Augmentation of granulocyte/macrophage colony-stimulating factor expression by ultraviolet irradiation is mediated by interleukin 1 in Pam 212 keratinocytes.  |  Nozaki, S., et al. 1991. J Invest Dermatol. 97: 10-4. PMID: 2056179
4. Identification of a novel thymocyte growth-promoting factor derived from B cell lymphomas.  |  Suda, T., et al. 1990. Cell Immunol. 129: 228-40. PMID: 2194678
5. GM-CSF: From Growth Factor to Central Mediator of Tissue Inflammation.  |  Becher, B., et al. 2016. Immunity. 45: 963-973. PMID: 27851925
6. Inhaled GM-CSF for Pulmonary Alveolar Proteinosis.  |  Tazawa, R., et al. 2019. N Engl J Med. 381: 923-932. PMID: 31483963
7. GM-CSF-Dependent Inflammatory Pathways.  |  Hamilton, JA. 2019. Front Immunol. 10: 2055. PMID: 31552022
8. IFNγ and GM-CSF control complementary differentiation programs in the monocyte-to-phagocyte transition during neuroinflammation.  |  Amorim, A., et al. 2022. Nat Immunol. 23: 217-228. PMID: 35102344
9. Therapeutic use of granulocyte-macrophage colony-stimulating factor (GM-CSF). A review of recent experience.  |  Costello, RT. 1993. Acta Oncol. 32: 403-8. PMID: 8369127
10. Granulocyte-macrophage colony-stimulating factor (GM-CSF) promotes phosphorylation and an increase in the activity of cytosolic phospholipase A2 in human neutrophils.  |  Nahas, N., et al. 1996. Biochem J. 313 (Pt 2): 503-8. PMID: 8573084
11. The role of GM-CSF in infection.  |  Freund, M. and Kleine, HD. 1992. Infection. 20 Suppl 2: S84-92. PMID: 1493940
12. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining.  |  Sander, B., et al. 1993. J Immunol Methods. 166: 201-14. PMID: 8288874

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GM-CSF Antibody (B6-2-hGMCSF) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-GM-CSF Antibody (B6-2-hGMCSF) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GM-CSF Antibody (B6-2-hGMCSF) Specifications

Alternate Product Name: Colony Stimulating Factor

Product Clone Name: B6-2-hGMCSF

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: GM-CSF

Epitope Species: human

Product Reactivity/Detection: GM-CSF

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for GM-CSF Antibody (B6-2-hGMCSF): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin