Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GlyR α2 Double Nickase Plasmid (h): sc-402179-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GlyR α2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GlyR α2 Double Nickase Plasmid (h) and GlyR α2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GLRA2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GlyR α2 Antibody (C-11): sc-398964
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GlyR α2 Double Nickase Plasmid (h) | sc-402179-NIC | 20 µg | $410.00 | |||
GlyR α2 Double Nickase Plasmid (h2) | sc-402179-NIC-2 | 20 µg | $410.00 |
GLRA2 encodes the glycine receptor alpha-2 (GlyR α2) subunit, a ligand-gated chloride channel of the Cys-loop receptor family that mediates fast inhibitory neurotransmission in the central nervous system. GlyR α2 contributes to synaptic and extrasynaptic glycinergic signaling that shapes neuronal excitability, network synchronization, and the balance between excitation and inhibition through chloride conductance. This receptor participates in neurodevelopmental processes including synapse maturation and circuit refinement, and its function intersects with pathways governing inhibitory synaptic transmission and ion homeostasis. Altered glycinergic signaling and GLRA2 sequence or expression changes have been linked in genetic and functional studies to neurodevelopmental phenotypes and seizure-related or excitability disorders, supporting its relevance for mechanistic neuroscience research.
GlyR α2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GLRA2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GLRA2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GLRA2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GLRA2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.