Date published: 2025-9-7

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GFP 항체 (15): sc-101525

4.8(12)
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데이터 시트
  • GFP 항체(15) 는 마우스 monoclonal IgG1κ, 24간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 재조합형에 대해 제기됨 GFP
  • IF와 IHC(P)으로 GFP 을 검출할것을 권장합니다.
  • 현재 GFP 항체(15)에 대해 선호하는 2차 검출 시약의 식별을 아직 완료하지 못했습니다. 이 작업은 진행 중입니다.

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녹색 형광 단백질(GFP)은 원래 해파리 Aequorea victoria의 생물 발광에 관여하는 단백질로 밝혀졌습니다. 외인성 기질이나 보조 인자 없이도 원핵세포에서 발현될 때 형광 생성물을 생성하는 GFP cDNA는 생체 내 유전자 발현과 단백질 위치를 모니터링하는 데 유용한 도구로 사용됩니다. 야생형 GFP보다 더 강렬하게 형광을 내는 EGFP를 비롯한 여러 GFP 돌연변이체가 개발되었습니다. 이러한 돌연변이체의 최대 여기치는 이중 표지 응용 분야뿐만 아니라 FACS 및 형광 현미경 검사에 더 유리합니다. GFP는 융합 단백질 태그로서 발현 벡터에 널리 사용되어 GFP에 융합된 이종 단백질의 발현 및 모니터링을 가능하게 합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

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제품명카탈로그 번호 단위가격수량관심품목

GFP 항체 (15)

sc-101525
200 µg/ml
$316.00

Is this antibody also recognizing YFP?

Asked by: Adrian31
Thank you for your question,. The antibody does not detect YFP.
Answered by: BlakeJ
Date published: 2023-08-09

You recommended the GFP Antibody (15): sc-101525 for detection of GFP by IHC(P) but I see no picture and cannot find any published data? Could you share your data or help me find published papers. I am OK to test a free sample of the antibody. Thx

Asked by: UCRes
Thank you for your question. We recommend this mouse monoclonal antibody, GFP (15): sc-101525, for detection of GFP by IF and IHC(P) based on publications in the literature. For one reference, please refer to the publication below on PubMed, PMID: 20099318, which is also listed as a product citation on our website (with a link out to PubMed). We are not currently offering a free sample size for this particular antibody. As an alternative, if you would like to test a free sample of GFP Antibody (B-2): sc-9996, please contact our Technical Service Department. This antibody is not currently recommended for IHC(P) because we have not yet tested the antibody with FFPE, however, it also has been published for this application and may be suitable.
Answered by: Technical Support
Date published: 2017-09-11

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with GFP Antibody (15): sc-101525?

Asked by: Trav11
Thank you for your question. We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 3
Date published: 2017-02-03
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Rated 5 out of 5 by from Great antibodyWorked very well with Western blotting and IP. Would highly recommend this antibody.
Date published: 2019-05-10
Rated 4 out of 5 by from GOODI had this one to test the CONSTRUCT, and it worked well in HEK lysate
Date published: 2018-05-14
Rated 5 out of 5 by from Really good antibodyGot this as sample.Now ordering it for future use.
Date published: 2017-12-16
Rated 5 out of 5 by from Good effectGFP used for detect tumor cell, IF of this antibody is obvious.
Date published: 2017-09-06
Rated 5 out of 5 by from Good Antibody!We used this antibody for IF staining and WB, and it has got great results with a nice clear image.
Date published: 2017-09-04
Rated 3 out of 5 by from One work, one didn'tfirst one I bought didn't work. Then the tech person sent me two more, same one I picked still not work. the other one he suggested worked.
Date published: 2017-03-23
Rated 5 out of 5 by from Great IF stainWe used this antibody for IF staining and got great results with a nice clear image
Date published: 2017-01-10
Rated 5 out of 5 by from This antibody is good for Western BlotI used GFP-tag antibody at a dilution of 1:2000 in PBST for 1 h and is enough for an excellent Western blot signal when the protein from DF-1 cell lysate.
Date published: 2016-11-24
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GFP 항체 (15) is rated 4.8 out of 5 by 12.
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