Gephyrin Antibody (B-4): sc-55469
- Gephyrin Antibody (B-4) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- raised against amino acids 437-736 of Gephyrin of rat origin
- recommended for detection of Gephyrin of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine and canine
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Gephyrin Antibody (B-4) for WB applications. These reagents are now offered in bundles with Gephyrin Antibody (B-4) (see ordering information below).
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Gephyrin Antibody (B-4) is a mouse monoclonal IgG2b antibody that detects Gephyrin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Gephyrin is a crucial microtubule-associated protein predominantly expressed in the brain, where Gephyrin plays a vital role in the postsynaptic localization of inhibitory glycine receptors, effectively anchoring these receptors to subsynaptic microtubules. This anchoring is essential for maintaining synaptic stability and regulating inhibitory neurotransmission, which is critical for proper neuronal function and communication. Gephyrin is not only expressed in the brain but is also found in various mammalian tissues, highlighting Gephyrin′s importance in diverse physiological processes. Additionally, at least five splice variants of Gephyrin have been identified in rat and human brain tissue, each potentially contributing to Gephyrin′s functional diversity and regulatory mechanisms in synaptic transmission. Anti-Gephyrin antibody (B-4) is an invaluable tool for researchers studying synaptic dynamics and the molecular underpinnings of neurotransmission.
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Gephyrin Antibody (B-4) References:
- Receptors, gephyrin and gephyrin-associated proteins: novel insights into the assembly of inhibitory postsynaptic membrane specializations. | Kneussel, M. and Betz, H. 2000. J Physiol. 525 Pt 1: 1-9. PMID: 10811719
- GABA(A) receptors, gephyrin and homeostatic synaptic plasticity. | Tyagarajan, SK. and Fritschy, JM. 2010. J Physiol. 588: 101-6. PMID: 19752109
- Gephyrin phosphorylation in the functional organization and plasticity of GABAergic synapses. | Zacchi, P., et al. 2014. Front Cell Neurosci. 8: 103. PMID: 24782709
- Gephyrin and the regulation of synaptic strength and dynamics at glycinergic inhibitory synapses. | Alvarez, FJ. 2017. Brain Res Bull. 129: 50-65. PMID: 27612963
- Structure-Function Relationships of Glycine and GABAA Receptors and Their Interplay With the Scaffolding Protein Gephyrin. | Kasaragod, VB. and Schindelin, H. 2018. Front Mol Neurosci. 11: 317. PMID: 30258351
- Binding of gephyrin to microtubules is regulated by its phosphorylation at Ser270. | Zhou, L., et al. 2021. Histochem Cell Biol. 156: 5-18. PMID: 33796945
- Gephyrin-Lacking PV Synapses on Neocortical Pyramidal Neurons. | Kuljis, DA., et al. 2021. Int J Mol Sci. 22: PMID: 34576197
- Deciphering the conformational dynamics of gephyrin-mediated collybistin activation. | Imam, N., et al. 2022. Biophys Rep (N Y). 2: 100079. PMID: 36425671
- Gephyrin phosphorylation facilitates sexually dimorphic development and function of parvalbumin interneurons in the mouse hippocampus. | Campbell, BFN., et al. 2024. Mol Psychiatry. 29: 2510-2526. PMID: 38503929
- Gephyrin promotes autonomous assembly and synaptic localization of GABAergic postsynaptic components without presynaptic GABA release. | Carricaburu, E., et al. 2024. Proc Natl Acad Sci U S A. 121: e2315100121. PMID: 38889143
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gephyrin Antibody (B-4) | sc-55469 | 200 µg/ml | $322.00 | |||
Gephyrin Antibody (B-4): m-IgG Fc BP-HRP Bundle | sc-537040 | 200 µg Ab; 10 µg BP | $361.00 | |||
Gephyrin Antibody (B-4): m-IgGκ BP-HRP Bundle | sc-534224 | 200 µg Ab; 40 µg BP | $361.00 | |||
Gephyrin Antibody (B-4): m-IgG2b BP-HRP Bundle | sc-549852 | 200 µg Ab; 10 µg BP | $361.00 |