Date published: 2025-9-6

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GATA3 항체 (HG3-31): sc-268

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데이터 시트
  • GATA3 항체 HG3-31 는 마우스 monoclonal IgG1 κ GATA3 항체, 425간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human origin의 GATA-3의 전체인 아미노산을 항원으로 사용하였습니다.
  • GATA3 항체 (HG3-31)는 WB, IP, IF and IHC(P)으로 mouse, rat, human and avian유래의 GATA-3 를 감지하는 데에 추천한다.
  • 항-GATA3 항체(HG3-31)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-268 X, 200 µg/0.1 ml)이 있습니다.
  • GATA3 (HG3-31): sc-268무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 는 GATA3 항체 (HG3-31) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은GATA3 항체(HG3-31) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다).

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GATA-3 항체(HG3-31)는 마우스, 쥐, 인간, 조류에서 유래된 GATA-3 단백질을 웨스턴 블롯(WB), 면역침전(IP), 면역형광(IF), 면역조직화학(IHC)을 통해 검출하는 마우스 단일클론 IgG1 카파 경쇄 항체입니다. GATA-3 단일 클론 항체(HG3-31)는 비접합 형태와 아가로스, 과산화효소, 피코에리트린, 플루오레세인 이소티오시아네이트, 여러 알렉사 플루오르® 접합체를 포함한 다양한 접합체 형태로 제공됩니다. GATA-3는 T 림프구의 발달과 기능에 중요한 역할을 하며, T 세포의 분화와 기능에 필수적인 유전자의 발현을 조절하는 전사 인자 역할을 합니다. GATA-3의 발현은 면역 반응에서 특히 중요합니다. GATA-3은 세포 외 병원체와 알레르기 반응에 대한 반응을 조율하는 데 중요한 Th2 세포의 발달에 관여하기 때문입니다. GATA-3은 주로 T세포의 핵에 위치하며, GATA-3은 특정 DNA 서열에 결합하여 유전자 발현을 조절합니다. 핵 내에서의 국소화는 기능에 필수적입니다. 표적 유전자의 전사에 직접적인 영향을 미치기 때문에 T세포의 발달과 기능을 촉진하기 때문입니다. 이러한 과정에 대한 GATA-3의 조절은 정상적인 면역 기능과 알레르기 및 자가면역질환을 포함한 다양한 질병의 발병 기전에서 그 중요성을 강조합니다. GATA-3 단일클론 항체(HG3-31)는 T세포 생물학과 면역 반응의 근본적인 메커니즘을 연구하는 연구자들에게 강력한 도구를 제공합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

GATA3 항체 (HG3-31) 참고문헌:

  1. 인간 전사인자 GATA-2. 내피 세포에서 프리프로엔도텔린-1 유전자 발현 조절에 대한 증거.  |  Dorfman, DM., et al. 1992. J Biol Chem. 267: 1279-85. PMID: 1370462
  2. CTLA-4는 세포당 GATA-3 단백질 수준을 조절하여 알레르겐 반응을 조절합니다.  |  Nasta, F., et al. 2007. Immunology. 121: 62-70. PMID: 17313444
  3. GATA-3 - 더 이상 Th2 세포만을 위한 것이 아닙니다.  |  Ho, IC. and Pai, SY. 2007. Cell Mol Immunol. 4: 15-29. PMID: 17349208
  4. GATA-3와 유방 세포의 운명.  |  Naylor, MJ. and Ormandy, CJ. 2007. Breast Cancer Res. 9: 302. PMID: 17381824
  5. 4-1BB는 T세포에서 IL-13 생산을 유발하여 양극화된 Th1 매개 염증을 제한합니다.  |  Shin, SM., et al. 2007. J Leukoc Biol. 81: 1455-65. PMID: 17389581
  6. 쥐와 인간 T-림프구 GATA-3 인자는 인간 T-세포 수용체 델타 유전자 인핸서 내의 시스 조절 요소를 통해 전사를 매개합니다.  |  Ko, LJ., et al. 1991. Mol Cell Biol. 11: 2778-84. PMID: 2017177
  7. 발달하는 심장과 장에서 전사되는 세 가지 전사 인자의 하위 계열인 GATA-4/5/6.  |  Laverriere, AC., et al. 1994. J Biol Chem. 269: 23177-84. PMID: 8083222
  8. GATA 전사인자 계열의 DNA 결합 특이성.  |  Ko, LJ. and Engel, JD. 1993. Mol Cell Biol. 13: 4011-22. PMID: 8321208
  9. 인간 GATA-6 유전자: 구조, 염색체 위치, 조직 특이적 및 미토겐 반응성 신호에 의한 발현 조절.  |  Suzuki, E., et al. 1996. Genomics. 38: 283-90. PMID: 8975704

주문정보

제품명카탈로그 번호 단위가격수량관심품목

GATA3 항체 (HG3-31)

sc-268
200 µg/ml
$316.00

GATA3 (HG3-31): m-IgG Fc BP-HRP 번들

sc-528106
200 µg Ab; 10 µg BP
$354.00

GATA3 (HG3-31): m-IgGκ BP-HRP 번들

sc-520404
200 µg Ab, 40 µg BP
$354.00

GATA3 (HG3-31): m-IgG1 BP-HRP 번들

sc-542762
200 µg Ab; 20 µg BP
$354.00

GATA3 항체 (HG3-31) X

sc-268 X
200 µg/0.1 ml
$316.00

GATA3 항체 (HG3-31) AC

sc-268 AC
500 µg/ml, 25% agarose
$416.00

GATA3 항체 (HG3-31) HRP

sc-268 HRP
200 µg/ml
$316.00

GATA3 항체 (HG3-31) FITC

sc-268 FITC
200 µg/ml
$330.00

GATA3 항체 (HG3-31) PE

sc-268 PE
200 µg/ml
$343.00

GATA3 항체 (HG3-31) Alexa Fluor® 488

sc-268 AF488
200 µg/ml
$357.00

GATA3 항체 (HG3-31) Alexa Fluor® 546

sc-268 AF546
200 µg/ml
$357.00

GATA3 항체 (HG3-31) Alexa Fluor® 594

sc-268 AF594
200 µg/ml
$357.00

GATA3 항체 (HG3-31) Alexa Fluor® 647

sc-268 AF647
200 µg/ml
$357.00

GATA3 항체 (HG3-31) Alexa Fluor® 680

sc-268 AF680
200 µg/ml
$357.00

GATA3 항체 (HG3-31) Alexa Fluor® 790

sc-268 AF790
200 µg/ml
$357.00

I have searched for good GATA3 antibody for liver tissues. I have tested several GATA3 antibody, the signals are not good. Does this antibody can meet my requirement?

Asked by: JUBOUWANG
Thank you for contacting Santa Cruz Biotechnology. GATA-3 (HG3-31) antibody has shown to work for liver please see pubmed IDs. We also have an antibody sample program for more information please email scbt@scbt.com. 19922796 36109558
Answered by: BlakeJ
Date published: 2024-11-22

I used this antigen in IF staining, but it did not work. I used heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). Should I change this buffer?

Asked by: TS10
Thank you for your question.Our recommended conditions for antigen retrieval are: 10 mM sodium citrate buffer, pH 6.0 (sc-294091); or with 50 mM glycine-HCl buffer (sc-29096), pH 3.5, with 0.01% (w/v) EDTA (sc-29092). Heat at 95° C for 5 minutes.Contact your dealer if you have any other questions.Thank you.
Answered by: Jiajun Z
Date published: 2023-11-20

Does it work on immunofluorescence of FFPE tissues (e.g. human lung and liver tissue)?

Asked by: rmmphd
Thank you for your question. Anti-GATA-3 Antibody (HG3-31) is recommended for detection of GATA-3 of mouse, rat, human and avian origin by WB, IP, IF and IHC(P) and will also work on FFPE-treated human lung and liver tissue samples.
Answered by: Tech Support Europe
Date published: 2022-07-12

What is the region of the amino acids (i.e. N-terminus or C-terminus?) in the recombinant human GATA-3 raised for this monoclonal antibody?

Asked by: Jay1980
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-11-30

We bought this antibody, GATA-3 Antibody (HG3-31): sc-268, and did IF on the sections ( 5 um) of mouse breast tumors after antigen unmasking (PH 6, pressure cooking 2 min). However, the location of GATA3 is in the cytoplasm, not in the nuclei. Why?

Asked by: Mmcc
Thank you for your question. Please contact our Technical Service Department for help with troubleshooting the antibody. Please call us toll-free at (800) 457-3801 option 2, or use the live chat on our website.
Answered by: Technical Support
Date published: 2018-11-22

Does the HG3-31 antibody show significant cross-reactivity with the other GATA factors (that is GATA-1, GATA-2, GATA-4, GATA-5 or GATA-6)?

Asked by: wliam
Thank you for your question. This mouse monoclonal, GATA-3 Antibody (HG3-31): sc-268, is specific for GATA-3 and does not cross-react with other GATA factors. The antibody is extremely well characterized, and has been cited in the literature over 300 times. If you would be interested in testing a 10 µg sample of this antibody, for a limited time, we are offering a sample size. Please contact our Technical Service Department either by phone at (800) 457-3801 option 2, email scbt@scbt.com, or use our live chat!
Answered by: Technical Support
Date published: 2018-09-10

may i ask that this antibody can detect GATA3 in pig?

Asked by: leseichi
Thank you for your question. We have not tested our mouse monoclonal, GATA-3 Antibody (HG3-31): sc-268, in pig. However, the antibody is raised against a recombinant protein immunogen that shares 94% sequence identity with the published protein for GATA-3 in pig (NP_001038032). Based on this sequence identity, we would expect this antibody to cross-react with GATA-3 in pig. If you are interested in testing a free sample of this antibody, please contact our Technical Service Department. As part of a limited time promotion, we are offering a free 10µg sample of our mouse monoclonal, GATA-3 Antibody (HG3-31): sc-268. Please call our Asian Support office toll-free at 800-820-8626, email asia@scbio.cn, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-02-01

Dilution and protocol to use on Dako Autostainer Link 48? Thanks

Asked by: joann
Thank you for your question. Our recommended immunohistochemistry protocol for using our monoclonal, GATA-3 Antibody (HG3-31): sc-268, is provided below. We have not used this antibody in combination with the Dako Autostainer Link 48 system, so we are unable to provide you any specific recommendations. However, this antibody has been validated for IHC on formalin-fixed paraffin-embedded tissue sections so it should be compatible. You may want to contact the other supplier to see if they can offer any specific recommendations for antibody usage with their equipment. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-01-25
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Rated 1 out of 5 by from immunofluorescenceI used it for IF at different concentration on human tissue and following all the suggestion but it didn't work.
Date published: 2024-08-23
Rated 1 out of 5 by from Not workingCannot get a specific band and got multiple unspecific bands.
Date published: 2020-10-08
Rated 1 out of 5 by from not workingI purchased this ab two weeks ago since was one of the best rated for GATA3. I tried BSA and MILK blocking, 1 to 200 dilution and increasing concentrations of MCF7 cell lysates. It did not work so far
Date published: 2018-10-29
Rated 4 out of 5 by from anti GATA3 sc-268anti GATA3 sc-268 , dilution 1/500 in human breast cancer cells
Date published: 2018-10-19
Rated 4 out of 5 by from Strong bandStrong band seen in WB using GATA-3 in whole cell lysate.
Date published: 2017-10-10
Rated 5 out of 5 by from GATA-3I used the antibody for immunofluroscence staining and it worked excellent
Date published: 2017-09-22
Rated 4 out of 5 by from GATA-3 wb is okayWB: 1:1000, mouse spleen tissue, 5% BSA block, antibody duluting with 5%BSA in TBST buffer. WB, two bind attached photos
Date published: 2017-09-02
Rated 4 out of 5 by from Good antibodyThe antibody works great for westernblotting using mouse brain tissue at 1:500 dilution.
Date published: 2017-07-24
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GATA3 항체 (HG3-31) is rated 4.0 out of 5 by 24.
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