Gastrin Double Nickase Plasmid (h): sc-403220-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Gastrin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Gastrin Double Nickase Plasmid (h) and Gastrin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GAST. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Gastrin Antibody (B-10): sc-28302
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gastrin Double Nickase Plasmid (h) | sc-403220-NIC | 20 µg | $410.00 | |||
Gastrin Double Nickase Plasmid (h2) | sc-403220-NIC-2 | 20 µg | $410.00 |
Human GAST encodes gastrin, a peptide hormone predominantly produced by gastric G cells that regulates gastric acid secretion and supports mucosal growth. After processing from preprogastrin into bioactive amidated gastrin peptides, it acts mainly through the CCKBR receptor to engage intracellular signaling including PLC/PKC and MAPK/ERK pathways that coordinate secretory activity, epithelial proliferation, and differentiation. Dysregulated gastrin production or signaling has been linked to gastric hypersecretion states and is frequently studied in the context of gastrointestinal inflammation, neuroendocrine tumor biology, and gastric and colorectal carcinogenesis. As a key node in gut endocrine signaling, GAST is widely used to model stimulus-dependent secretion programs and receptor-coupled transcriptional responses in gastrointestinal systems.
Gastrin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GAST locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GAST. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GAST function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GAST-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.