GAP-43 Antibody (B-5): sc-17790

GAP-43 Antibody (B-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects GAP-43 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). GAP-43 Antibody (B-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GAP-43, also known as growth-associated protein 43, plays a vital role in neuronal development and regeneration, being predominantly expressed in neuronal growth cones during these processes. GAP-43 presence is crucial for the formation of synaptic connections and the overall regenerative response in the nervous system. GAP-43 is involved in the modulation of synaptic plasticity, which is essential for learning and memory. GAP-43 interaction with specific RNA-binding proteins, such as the neuron-specific Elav/Hu family member HuD, enhances the stability of GAP-43 mRNA, thereby increasing GAP-43 levels in developing neurons. This regulation is particularly important, as studies have shown that heterozygous GAP-43 knockout mice, which exhibit a 50% reduction in GAP-43 levels, demonstrate significant impairments in memory tasks, highlighting GAP-43′s critical role in cognitive functions.

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GAP-43 Antibody (B-5) References:

1. Role of oleic acid as a neurotrophic factor is supported in vivo by the expression of GAP-43 subsequent to the activation of SREBP-1 and the up-regulation of stearoyl-CoA desaturase during postnatal development of the brain.  |  Velasco, A., et al. 2003. Brain Res. 977: 103-11. PMID: 12788519
2. Increase of the RNA-binding protein HuD and posttranscriptional up-regulation of the GAP-43 gene during spatial memory.  |  Pascale, A., et al. 2004. Proc Natl Acad Sci U S A. 101: 1217-22. PMID: 14745023
3. Superior olivary contributions to auditory system plasticity: medial but not lateral olivocochlear neurons are the source of cochleotomy-induced GAP-43 expression in the ventral cochlear nucleus.  |  Kraus, KS. and Illing, RB. 2004. J Comp Neurol. 475: 374-90. PMID: 15221952
4. Expression levels of cytoskeletal proteins indicate pathological aging of S100B transgenic mice: an immunohistochemical study of MAP-2, drebrin and GAP-43.  |  Shapiro, LA. and Whitaker-Azmitia, PM. 2004. Brain Res. 1019: 39-46. PMID: 15306236
5. GAP-43 mRNA in growth cones is associated with HuD and ribosomes.  |  Smith, CL., et al. 2004. J Neurobiol. 61: 222-35. PMID: 15389607
6. Neuromodulin (GAP43): a neuronal protein kinase C substrate is also present in 0-2A glial cell lineage. Characterization of neuromodulin in secondary cultures of oligodendrocytes and comparison with the neuronal antigen.  |  Deloulme, JC., et al. 1990. J Cell Biol. 111: 1559-69. PMID: 2170423
7. Differential changes in GAP-43 or synaptophysin during appetitive and aversive taste memory formation.  |  Grijalva, LE., et al. 2021. Behav Brain Res. 397: 112937. PMID: 32991926
8. Elevated CSF GAP-43 is associated with accelerated tau accumulation and spread in Alzheimer's disease.  |  Franzmeier, N., et al. 2024. Nat Commun. 15: 202. PMID: 38172114
9. Distribution of phosphorylated GAP-43 (neuromodulin) in growth cones directly reflects growth cone behavior.  |  Dent, EW. and Meiri, KF. 1998. J Neurobiol. 35: 287-99. PMID: 9622012
10. The neuronal growth-associated protein GAP-43 interacts with rabaptin-5 and participates in endocytosis.  |  Neve, RL., et al. 1998. J Neurosci. 18: 7757-67. PMID: 9742146
11. Association of GAP-43 with detergent-resistant membranes requires two palmitoylated cysteine residues.  |  Arni, S., et al. 1998. J Biol Chem. 273: 28478-85. PMID: 9774477
12. Randomized retinal ganglion cell axon routing at the optic chiasm of GAP-43-deficient mice: association with midline recrossing and lack of normal ipsilateral axon turning.  |  Sretavan, DW. and Kruger, K. 1998. J Neurosci. 18: 10502-13. PMID: 9852588

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GAP-43 Antibody (B-5) has 63 citations in a variety of scientific publications.

Primary antibodies like Anti-GAP-43 Antibody (B-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GAP-43 Antibody (B-5) Specifications

Alternate Product Name: GAP-43

Product Clone Name: B-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-100

Epitope Target: GAP-43

Epitope Species: human

Product Reactivity/Detection: axonal membrane protein GAP-43

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 63 citations in various scientific publications

Available Conjugates/Forms for GAP-43 Antibody (B-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin