FPR2 항체 (GM1D6) WB, IP 와FCM으로 human origin의 FPR2 transiently expressed on the cell surface of transfected BOSC cells.을 검출할것을 권장합니다.

FPR2 항체 (GM1D6): sc-57141

FPR2 항체 (GM1D6) is rated 4.6 out of 5 by 5.
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Datasheets

    • 안티-FPR2 항체 GM1D6 는 마우스 monoclonal IgG1 (kappa light chain) FPR2 항체 이며 200 µg/ml으로 제공합니다.
    • 유전자침묵제품은 비영리적인용도나 연구에만 사용가능합니다 자세한 정보보기.
    • FPR2 항체 (GM1D6)는 WB, IP and FCM으로 human유래의 FPR2 transiently expressed on the cell surface of transfected BOSC cells. 를 감지하는 데에 추천한다 .
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-FPR2 항체 (GM1D6)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • 강력추천하는 conventional secondary anti-mouse IgG reagents의 대체제품 보기m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP).
    • FPR2 (GM1D6): sc-57141무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
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Boa noite, adquirimos um anticorpo monoclonal FPR2 SC-57141 GM1D6 LOTE #E0217, queremos saber se ele é conjugado a fluoresceína.

Asked by: Maurício420
Thank you for your question. The antibody with catalog number: sc-57141, lot# E0217, is the unconjugated antibody. We do offer this antibody directly conjugated to fluorescein, and the catalog number is sc-57141 FITC.
Answered by: Technical Support
Date published: 2019-08-19

Good nigth, we got a monoclonal antibody FPR2 SC-57141 GM1D6 LOT # E0217, we want to know if it is conjugated to fluorescein.

Asked by: Maurício420
Thank you for your question. That specific catalog and lot number is not conjugated to fluorescein (FITC). The FITC conjugated antibody has the catalog number: sc-57141 FITC.
Answered by: Technical Support
Date published: 2019-06-15

Can FPR2 (GM1D6): sc-57141 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, FPR2 (GM1D6): sc-57141 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Quite good antibody We tested FPR2 by western blotting analysis. 60 micrograms of mouse brain membrane lysates were resolved on 10% SDS-PAGE, and incubated over night with monoclonal FPR2 antibody (1:1000). Secondary antibody utilized was anti-mouse sc-2005 antibody (1:5000). FPR2 signal was detected by chemidoc (Biorad) and the signal (not fully clear) was reached after medium exposition.
Date published: 2017-07-25
Rated 5 out of 5 by from Reducing or Non-reducing! Great detection of FPR2 in Western Blot under both reducing and non-reducing conditions!
Date published: 2017-01-17
Rated 4 out of 5 by from The antibody worked pretty good on freshly The antibody worked pretty good on freshly isolated human monocytes and granulocytes, when blocked with 5% heat-inactivated FCS. At a dilution of 1/1000 I observed clear stainings with minimal background and no signal for the isotype control. Tests for cross reactivity in HEK cells expressing hFPR1 or hFPR3 were negative. For high-quality pictures cell had to be fixated with 3% methanol-free paraformaldehyde. In summary the antibody works quite good with good specificity so far.
Date published: 2016-04-13
Rated 5 out of 5 by from Good specificity in WB in AML Good specificity in WB in AML-193 whole cell lysate. -SCBT QC
Date published: 2015-06-14
Rated 5 out of 5 by from Robust signal in WB in human PBL and AML Robust signal in WB in human PBL and AML-193 whole cell lysates. -SCBT QC
Date published: 2014-07-25
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