FOXE3 Double Nickase Plasmid (h): sc-404466-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FOXE3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FOXE3 Double Nickase Plasmid (h) and FOXE3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FOXE3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FOXE3 Antibody (G-1): sc-393526
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FOXE3 Double Nickase Plasmid (h) | sc-404466-NIC | 20 µg | $410.00 | |||
FOXE3 Double Nickase Plasmid (h2) | sc-404466-NIC-2 | 20 µg | $410.00 |
FOXE3 encodes a forkhead box transcription factor that is essential for anterior segment and lens development, where it regulates gene expression programs controlling proliferation, differentiation, and maintenance of lens epithelial cell identity. Through DNA-binding via its forkhead domain, FOXE3 influences developmental transcriptional networks and cell-cycle–linked processes that shape ocular morphogenesis and preserve tissue transparency. Dysregulation or loss of FOXE3 function is associated with congenital eye disorders, including anterior segment dysgenesis and cataract phenotypes, making it a relevant target for studying human ocular development and disease mechanisms. FOXE3 is therefore widely used as a marker and regulator in models of lens biology, transcriptional control, and developmental genetics.
FOXE3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FOXE3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FOXE3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FOXE3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FOXE3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.