Date published: 2025-9-7
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E-cadherin Antibody (67A4) - Western Blotting - Image 348906
E-카데린 항체(67A4): sc-21791. A431 전체 세포 용해물에서 E-카데린 발현의 웨스턴 블롯 분석.
E-카데린 항체(67A4): sc-21791. A431 전체 세포 용해물에서 E-카데린 발현의 웨스턴 블롯 분석.

E-cadherin 항체 (67A4): sc-21791

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데이터 시트
  • E-cadherin 항체(67A4) 는 마우스 monoclonal IgG1κ, 215간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human 유방암 세포주 T471에 대해 양성 반응
  • E-cadherin 항체 (67A4)는 WB, IP, IF and FCM으로 human유래의 E-cadherin 를 감지하는 데에 추천한다 .
  • 항-E-cadherin 항체(67A4)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • E-cadherin (67A4): sc-21791의 무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP, 1 BP-HRP">m-IgG1 BP-HRP 및 m-IgGκ BP-HRP는 E-cadherin 항체(67A4) WB 애플리케이션용입니다. 이 시약은 이제 E-cadherin 항체(67A4)와 함께 번들로 제공됩니다(아래 주문 정보 참조).

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추가 Cadherin Antibodies 포함 cadherin-16, cadherin-19, cadherin-23, E-cadherin, EY-cadherin, K-cadherin, LI-cadherin, M-cadherin, N-cadherin, OB-cadherin, P-cadherin, pan-cadherin, R-cadherin, T-cadherin 및 VE-cadherin

E-카데린 항체(67A4)는 웨스턴 블롯(WB), 면역침전법(IP), 면역형광법(IF), 유세포 분석법(FCM)을 통해 인간 E-카데린을 검출하는 마우스 단일클론 IgG1 카파 경쇄 항체입니다. 항-E-카데린 항체(67A4)는 비접합형과 아가로스, 호스래디시 퍼옥시다제(HRP), 피코에리트린(PE), 플루오레세인 이소티오시아네이트(FITC), 다중 알렉사 플루오르® 접합체를 포함한 다양한 접합형 형태로 제공됩니다. 카디닌 계열의 일원인 E-카디닌은 세포-세포 접착에 중요한 역할을 담당하며, 조직의 완전성을 유지하고 발달 과정에서 형태 발생 과정을 촉진하는 데 필수적입니다. E-카데린은 주로 세포막에 국한되어 인접한 세포를 연결하는 접합부(adherens junction)를 형성하고 조직의 구조적 안정성에 기여합니다. 이러한 막 국소화는 E-카데린이 상피층 형성과 암세포의 침습적 행동 방지에 필요한 강력한 세포 간 유착을 매개할 수 있게 합니다. β-카테닌과 같은 세포질 단백질과 E-카데린의 상호작용은 세포 증식과 분화를 조절하는 신호 전달 경로에 매우 중요하며, 정상적인 생리학 및 질병 상태 모두에서 그 중요성을 강조합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

E-cadherin 항체 (67A4)

sc-21791
200 µg/ml
$316.00

E-cadherin (67A4): m-IgG Fc BP-HRP 번들

sc-528370
200 µg Ab; 10 µg BP
$354.00

E-cadherin (67A4): m-IgGκ BP-HRP 번들

sc-520732
200 µg Ab, 40 µg BP
$354.00

E-cadherin (67A4): m-IgG1 BP-HRP 번들

sc-542898
200 µg Ab; 20 µg BP
$354.00

E-cadherin 항체 (67A4) AC

sc-21791 AC
500 µg/ml, 25% agarose
$416.00

E-cadherin 항체 (67A4) HRP

sc-21791 HRP
200 µg/ml
$316.00

E-cadherin 항체 (67A4) FITC

sc-21791 FITC
200 µg/ml
$330.00

E-cadherin 항체 (67A4) PE

sc-21791 PE
200 µg/ml
$343.00

E-cadherin 항체 (67A4) Alexa Fluor® 488

sc-21791 AF488
200 µg/ml
$357.00

E-cadherin 항체 (67A4) Alexa Fluor® 546

sc-21791 AF546
200 µg/ml
$357.00

E-cadherin 항체 (67A4) Alexa Fluor® 594

sc-21791 AF594
200 µg/ml
$357.00

E-cadherin 항체 (67A4) Alexa Fluor® 647

sc-21791 AF647
200 µg/ml
$357.00

E-cadherin 항체 (67A4) Alexa Fluor® 680

sc-21791 AF680
200 µg/ml
$357.00

E-cadherin 항체 (67A4) Alexa Fluor® 790

sc-21791 AF790
200 µg/ml
$357.00

Dear Santa Cruz, I am sending this message to ask if this antibody has been tested on mouse samples i.e. mouse reactivity. In addition, I read a lot of reviews with unsatisfactory results. Is there a reason? Thank you for getting back to me, Patrick

Asked by: patrikaouad
Thank you for your question. This antibody has not been tested for mouse and rat use, and can therefore not be warrantied for this use. If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-02-25

Dear Sir/Madame, I am writing this letter because I would like to try your product with the next specification: E-cadherin Antibody (67A4) Alexa Fluor® 647 sc-21791 AF647 200 µg/ml. Would you be so kind as to send me a trial size? Best regards, Babi

Asked by: Babi
Thank you for your question. We do not offer sample sizes of the conjugated primary antibodies. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this unconjugated monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-12

Is it possible to get free sample for testing before I order the antibody?

Asked by: Sammy123
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2024-05-27

Dear All Would it be possible to have sample of this antibody to test? Thank you

Asked by: fafv
Thank you for your question. Yes, for a limited time we are offering a free 10 µg sample of this mouse monoclonal, sc-21791: E-cadherin (67A4). Please contact a Technical Service Representative to request a sample. Please call our European Support office toll-free at 00800 4573 8000, email europe@scbt.com, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-03-16

Can I use the E-cadherin Antibody (67A4) for detection in bovine tissue samples?

Asked by: Trav11
To inquire about additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website. We will be more than happy to run sequence analysis for your species of interest.
Answered by: Tech Service 8
Date published: 2016-12-22
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Rated 5 out of 5 by from Westerblot/ImmunofluorescenceAntibody works very well in Westerblot and immunofluorescence (1:200, or 1:100 dilution, respectively) in airway epithelial cell lysate, or airway epithelial cell monolayers, respectively.
Date published: 2023-02-06
Rated 4 out of 5 by from It works!This antibody gave a strong and clean signal when I used it at a 1:200 diluition after o.n. incubation with 80 μg of proteins.
Date published: 2022-03-18
Rated 1 out of 5 by from Not goodI used the primary antibody 1:50 by IF on epithelial renal cells. Unfortunately, the signal was not detectable.
Date published: 2022-02-08
Rated 1 out of 5 by from Anticorpo non funzionantePrimary antibodies E-Cadherin (67A4) sc-21791 was used diluted 1:500 according to the manufacturer’s recommendations (for Western Blotting dilution range 1:100-1:1000), in Milk 5%. Membrane of PVDF has been incubated overnight at 4°C and then it has been probed for 1h at RT with Santa Cruz secondary antibodies diluted 1:5000 in Milk 1%. We have tested this antibody on two different sets of samples: in six cancer cell lines and in human activated PBMCs. Moreover, even if we tested the antibody at higher concentration, we only obtained a higher background but not an increase in the specific protein signal; therefore we show only the blots obtained after the incubation with antibody prepared as described above. In conclusion: • E-Cadherin did not work at all, neither after a long exposure. We do not have tools to verify the specificity for this antibody too. All the membranes have been probed with a normalizer and each gel has been stained with Coomassie Brillant Blue, shown on the right side of each panel, in order to demonstrate that the samples ran well and that the proteins are present on the SDS-PAGE.
Date published: 2019-11-26
Rated 5 out of 5 by from Greatit is useful to our purpose and efficiency.
Date published: 2019-10-17
Rated 1 out of 5 by from Not working at allThis antibody does not work in even 1:100 concentration neither in WB nor IF
Date published: 2018-11-30
Rated 1 out of 5 by from FACSThis antibody does not work on HFFF cells in flow cytometry.
Date published: 2018-11-01
Rated 1 out of 5 by from Not workingDoes not work in western and IF of breast cancer cell lines.
Date published: 2018-10-12
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E-cadherin 항체 (67A4) is rated 3.8 out of 5 by 31.
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