DDEF1 Antibody (E-12): sc-374411
- DDEF1 Antibody (E-12) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 837-873 near the C-terminus of DDEF1 of human origin
- recommended for detection of DDEF1 of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG1 BP-HRP is the preferred secondary detection reagent for DDEF1 Antibody (E-12) for WB applications. This reagent is now offered in a bundle with DDEF1 Antibody (E-12) (see ordering information below).
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DDEF1 Antibody (E-12) is a mouse monoclonal IgG1 antibody that detects DDEF1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DDEF1, also known as development and differentiation enhancing factor 1, ASAP1, AMAP1, or PAG2, is a crucial ADP ribosylation factor (ARF)-GTPase activating protein (GAP) that plays a significant role in cellular signaling pathways. DDEF1 is primarily localized in the cytoplasm and at newly formed focal complexes at the cell periphery, where DDEF1 interacts with key signaling proteins such as ARF1, ARF5, ARF6, and SRK (ZAP-70). These interactions are vital for regulating cell motility and actin cytoskeletal remodeling, which are essential processes for cell growth and differentiation, particularly in adipocytes and fibroblasts. DDEF1′s structure includes two ankyrin repeats, one ARF-GAP domain, one SH3 domain, and one PH domain, the latter being critical for the phosphoinositide-dependent regulation of ARFs. Notably, overexpression of DDEF1 has been associated with the inhibition of invasion and metastasis in breast cancer and high-grade uveal melanomas, highlighting DDEF1′s potential as a therapeutic target and diagnostic marker in oncology. DDEF1 monoclonal antibody (E-12) is an invaluable tool for researchers investigating DDEF1′s roles in cancer biology and cellular differentiation.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDEF1 Antibody (E-12) | sc-374411 | 200 µg/ml | $322.00 | |||
DDEF1 Antibody (E-12): m-IgG1 BP-HRP Bundle | sc-542699 | 200 µg Ab; 20 µg BP | $361.00 | |||
DDEF1 (E-12) Neutralizing Peptide | sc-374411 P | 100 µg/0.5 ml | $69.00 |