D5DR Antibody (E-12): sc-376088

D5DR Antibody (E-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects D5DR protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-D5DR antibody (E-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. D5DR protein, also known as dopamine receptor D5, DR1B, or DRD1L2, is a G protein-coupled receptor belonging to the D1-like family, which plays a crucial role in stimulating adenylyl cyclase and exhibits a tenfold higher affinity for dopamine compared to the D1 subtype. D5DR is predominantly located in neurons within the limbic regions of the brain, with mRNA being most abundant in specific cortical areas (layers II, IV, and VI), the dentate gyrus, and various hippocampal subfields, while showing lower expression levels in the striatum. D5DR′s strategic location is vital for modulating neurotransmission and influencing behaviors associated with motivation, reward, and cognition. Five major types of dopamine receptors exist, all G protein-coupled metabotropic receptors that can exert either excitatory or inhibitory effects on post-synaptic neurons. D1 (D1A, D1C, D1D) and D5 (D1B) receptors are part of the D1-like receptor family, which typically promotes excitatory signaling through increases in cyclic adenosine monophosphate (cAMP), while D2, D3, and D4 receptors belong to the D2-like family, which generally inhibits cAMP production. Research has shown a significant age-related decline in dopamine receptor mRNAs, particularly in the hippocampus and entorhinal cortex, highlighting D5DR′s relevance in neurobiological studies and potential therapeutic targets for age-related cognitive decline.

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D5DR Antibody (E-12) References:

1. Dopamine receptor localization in the mammalian retina.  |  Nguyen-Legros, J., et al. 1999. Mol Neurobiol. 19: 181-204. PMID: 10495103
2. Delineation of the structural basis for the activation properties of the dopamine D1 receptor subtypes.  |  Iwasiow, RM., et al. 1999. J Biol Chem. 274: 31882-90. PMID: 10542214
3. Immunoblot and immunohistochemical comparison of murine monoclonal antibodies specific for the rat D1a and D1b dopamine receptor subtypes.  |  Luedtke, RR., et al. 1999. J Neuroimmunol. 101: 170-87. PMID: 10580800
4. Quantification of D1B(D5) receptors in dopamine D1A receptor-deficient mice.  |  Montague, DM., et al. 2001. Synapse. 39: 319-22. PMID: 11169782
5. D1 dopamine receptors in the mouse prefrontal cortex: Immunocytochemical and cognitive neuropharmacological analyses.  |  Lidow, MS., et al. 2003. Synapse. 47: 101-8. PMID: 12454947
6. Insight into the mechanism of dopamine D1-like receptor activation. Evidence for a molecular interplay between the third extracellular loop and the cytoplasmic tail.  |  Tumova, K., et al. 2003. J Biol Chem. 278: 8146-53. PMID: 12509438
7. Delineation of the conserved functional properties of D1A, D1B and D1C dopamine receptor subtypes in vertebrates.  |  Le Crom, S., et al. 2004. Biol Cell. 96: 383-94. PMID: 15207908
8. Expression of functional receptors and transmitter enzymes in cultured Muller cells.  |  Kubrusly, RC., et al. 2005. Brain Res. 1038: 141-9. PMID: 15757630
9. Dopamine receptor D5 deficiency results in a selective reduction of hippocampal NMDA receptor subunit NR2B expression and impaired memory.  |  Moraga-Amaro, R., et al. 2016. Neuropharmacology. 103: 222-35. PMID: 26714288
10. Dopamine Receptor D5 Signaling Plays a Dual Role in Experimental Autoimmune Encephalomyelitis Potentiating Th17-Mediated Immunity and Favoring Suppressive Activity of Regulatory T-Cells.  |  Osorio-Barrios, F., et al. 2018. Front Cell Neurosci. 12: 192. PMID: 30042660
11. Dopamine Receptor D5 is a Modulator of Tumor Response to Dopamine Receptor D2 Antagonism.  |  Prabhu, VV., et al. 2019. Clin Cancer Res. 25: 2305-2313. PMID: 30559168
12. The human dopamine D5 receptor gene: cloning and characterization of the 5'-flanking and promoter region.  |  Beischlag, TV., et al. 1995. Biochemistry. 34: 5960-70. PMID: 7727453

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-D5DR Antibody (E-12) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-D5DR Antibody (E-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

D5DR Antibody (E-12) Specifications

Alternate Product Name: D5DR

Product Clone Name: E-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 371-477

Epitope Target: D5DR

Epitope Species: human

Product Reactivity/Detection: D5DR

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for D5DR Antibody (E-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred D5DR antibody is D5DR Antibody (SG4-D1b): sc-33661.