D-Luciferin, sodium salt monohydrate (CAS 103404-75-7)

사용법 :

Preparation of stock solutions:

Dissolve 1 g of D-Luciferin sodium salt in 12.5 ml of PBS to get a final stock solution of 80 mg/ml or dissolve 1 g of D-Luciferin sodium salt in 33.3 ml of molecular biology grade water to get a final stock solution of 30 mg/ml. Use immediately or store in aliquots at -20°C.

Preparation of Luciferin for In Vitro Bioluminescent Assay:

Prepare a 150 μg/mL working solution of luciferin in a tissue culture medium, draw off media from cultured cells and add luciferin to cells just before imaging.

모습 :

Powder

건강 상태 :

Solid

용해도 :

Soluble in water (1 %), and 0.05 M glycine (4 mg/ml).

저장 :

Desiccate at -20° C

굴절률 :

n²⁰_D ~1.86 (Predicted)

형광 :

λex 328 nm, λem 532 nm in water; λex 327 nm, λem 537 nm in pH 4; λex 385 nm, λem 537 nm in pH 11

PubChem CID :

Merck 색인 :

12: 4128

MDL 번호 :

MFCD11865368

SMILES :

C1[C@@H](NC(=C2N=C3C=CC(=O)C=C3S2)S1)C(=O)[O-].[Na+].O