Cytokeratin 19 항체 (A53-B/A2) WB, IP, IF 와IHC(P)으로 human origin의 amino acids 312-335 of Cytokeratin 19을 검출할것을 권장합니다.

Cytokeratin 19 항체 (A53-B/A2): sc-6278

Cytokeratin 19 항체 (A53-B/A2) is rated 5.0 out of 5 by 10.
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데이터 시트

    • Cytokeratin 19 항체(A53-B/A2) 는 마우스 monoclonal IgG2aκ, 82간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • raised against breast carcinoma cell line MCF7 of human origin
    • Cytokeratin 19 항체 (A53-B/A2)는 WB, IP, IF and IHC(P)으로 human유래의 amino acids 312-335 of Cytokeratin 19 를 감지하는 데에 추천한다 .
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Cytokeratin 19 항체 (A53-B/A2)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Cytokeratin 19 (A53-B/A2): sc-6278무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Cytokeratin 19 Antibody (A53-B/A2) for WB and IHC(P) applications. These reagents are now offered in bundles with Cytokeratin 19 Antibody (A53-B/A2) (see ordering information below).
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Which antigen retrieval technique is needed for IHC-P ? thanks

Asked by: SophiePaquet
Thank you for your question. We recommend using sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval.
Answered by: Technical Support
Date published: 2019-06-16

What is the recommended secondary reagent to use with this mouse monoclonal primary antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 5
Date published: 2017-01-11

Can this antibody be used for detection of Cytokeratin 19 of mouse origin?

Asked by: AbPolly
Thank you for your question. This antibody has not been validated for use to detect Cytokeratin 19 of mouse origin. For further details and sequence analysis, please contact our Technical Service Department.
Answered by: Technical Support 5
Date published: 2017-01-11
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Rated 5 out of 5 by from 美しいバンドが検出されます。 WBで使用しました。シャープなバンドが検出されました。非特異的なバンドは一切ありません。バンドの大きさもデータシートと同じ位置です。
Date published: 2020-12-01
Rated 5 out of 5 by from Works well Used the antibody for IP and WB and worked well in both cases
Date published: 2020-03-09
Rated 5 out of 5 by from Good on FFPE I used this antibody on formalin-fixed paraffin-embedded human carcinomas. The staining was quite decent. The antibody was used at a 1:30 dilution and incubated for 15 minutes. Staining was clear. I also used CK19 (A-3) antibody on a serial section simultaneously and although both antibodies were quite good, the staining from CK19 (A-3) was more vibrant.
Date published: 2017-08-10
Rated 5 out of 5 by from Just a great product Strong western blot data; also a superb product for western applications
Date published: 2017-01-29
Rated 5 out of 5 by from Good antibody The Cytokeratin 19 Antibody (A53-B/A2): sc-6278 works very well for IHC with formalin fixed, paraffin-embedded tissue sections showing localized staining to the cytoplasm and membrane. This antibody has also been cited over 40 times for further verification.
Date published: 2017-01-16
Rated 5 out of 5 by from IHC publication with human breast cancer IHC publication with human breast cancer. -SCBT Publication Review
Date published: 2015-02-23
Rated 5 out of 5 by from Beautiful IHC data in paraffin Beautiful IHC data in paraffin-embedded human breast and pancreas tissue. -SCBT QC
Date published: 2015-02-17
Rated 5 out of 5 by from Immunofluorescence publication with human liver Immunofluorescence publication with human liver. -SCBT Publication Review
Date published: 2015-02-10
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