cyclin B1 항체 (GNS1) WB, IP, IF와 IHC(P)으로 mouse, rat와 human origin의 cyclin B1을 검출할것을 권장합니다.

cyclin B1 항체 (GNS1): sc-245

cyclin B1 항체 (GNS1) is rated 4.6 out of 5 by 42.
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데이터 시트

    • 안티-cyclin B1 항체 GNS1 는 마우스 monoclonal IgG1 κ cyclin B1 항체, 1,134간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • raised against a recombinant protein corresponding to human cyclin B1
    • 안티-cyclin B1 항체 (GNS1)는 WB, IP, IF and IHC(P)으로 mouse, rat and human유래의 cyclin B1 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-cyclin B1 항체 (GNS1)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • IF, IHC(P) 와 FCM에 사용가능한 TRITC or Alexa Fluor® 405 결합제품이 있습니다.
    • ChIP 에 사용하는 TransCruz시약(sc-245 X, 200 µg/0.1 ml)이 있습니다.
    • cyclin B1 (GNS1): sc-245무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for cyclin B1 Antibody (GNS1) for WB and IHC(P) applications. These reagents are now offered in bundles with cyclin B1 Antibody (GNS1) (see ordering information below).
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Hi, do you already know if this antibody will react with bovine cells/tissue?

Asked by: clvleal
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Hi, do you know if the epitope locates on the N-terminal region or the C-terminal region? Thanks!

Asked by: NancySL
Thank you for your questions. Anti-cyclin B1 Antibody clone GNS1 has been reported to recognize an epitope in the N-terminus region of human cyclin B1.
Answered by: Santa Cruz TS
Date published: 2021-07-05

Will this antibody react with bovine protein? Than you.

Asked by: clvleal
Thank you for your question. We are aware of one publication citing use of this antibody in bovine ABAE cells. Please refer to this reference on NCBI's PubMed, PMID: 15746189.
Answered by: Technical Support
Date published: 2017-08-02

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Sweetums
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 4
Date published: 2017-11-03

I need to use an antibody to detect the protein in samples of (other species, e.g., bovine, porcine, canine, equine, avian, non-mammalian) origin. Do you know if this mouse monoclonal antibody will work?

Asked by: Sweetums
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 4
Date published: 2016-12-16
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Rated 4 out of 5 by from good antibody good antibody clean and strong signal in western blot or IF
Date published: 2021-11-15
Rated 5 out of 5 by from Very good antibody We used in WB at 1:200 dilution on K562 whole cell lysates. We obtained a strong and specific signals.
Date published: 2021-07-09
Rated 4 out of 5 by from Good for western blot The antibody can detect a specific band with 1:250 dilution for western blot. Samples were HeLa cell extract.
Date published: 2019-10-08
Rated 5 out of 5 by from anti-cyclin B1 antibody works for western blot. We used the anti-cyclin B1 antibody for western blotting of HeLa whole cell lysate and detected a band of the correct size.
Date published: 2019-07-29
Rated 1 out of 5 by from No Western signal in INS-1 rat cell lysates Tested by Western Blot (Li-Cor reagents) of rat insulinoma (INS-1) cell line. 60 ug loads were probed overnight, but no bands were detected. Alternatively, faint bands detected using sc-7393
Date published: 2019-05-22
Rated 5 out of 5 by from Figure 5-D Protein analysis by Western blot analysis of CCNB1 in three mutant lung adenocarcinoma cells (H1792.604, H2009, H358)
Date published: 2019-02-22
Rated 5 out of 5 by from wonderful antibody! 1:200 diluted for wb of rat protein ,the band is strong and special
Date published: 2018-06-12
Rated 5 out of 5 by from Good for WB This antibody is good for Western blot using human cell nuclear extract.
Date published: 2018-05-12
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