human origin의 CPTII에서 내부에 위치한 51-350 아미노산을 항원으로 사용하였습니다.
안티-CPTII 항체 (G-5)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 CPTII 를 감지하는 데에 추천한다.
IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-CPTII 항체 (G-5)를 제공한다.
WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for CPTII Antibody (G-5) for WB applications. These reagents are now offered in bundles with CPTII Antibody (G-5) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
CPTII Antibody (G-5) is a high quality monoclonal CPTII antibody (also designated CPT2 antibody, carnitine/choline acetyltransferase antibody, carnitine palmitoyltransferase II antibody, PT1 antibody, CPTASE antibody, or infection-induced acute encephalopathy-4 (IIAE4) antibody) suitable for the detection of the CPTII protein of mouse, rat and human origin. CPTII Antibody (G-5) is available as both the non-conjugated anti-CPTII antibody form, as well as multiple conjugated forms of anti-CPTII antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The mitochondrial β-oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase (CPT) I (outer membrane and detergent labile) and II (inner membrane and detergent stable), together with carnitine carrier. CPTI catalyzes the first reaction in the transport of long-chain fatty acids from the cytoplasm to the mitochondrion, a rate-limiting step in beta-oxidation. Two types of CPTI are known, the liver (CPTIA) and muscle (CPTIB) isoforms. The muscle type protein is specially expressed in heart and skeletal muscle. Membrane-bound CPTI, but not CPTII, is inhibited reversibly by malonyl-coenzyme A (CoA). Unlike CPTII, CPTI requires membrane integrity for catalytic function. In addition, glutamic acid 3 and histidine 5 are necessary for malonyl CoA inhibition and binding to liver CPTI, but not for catalytic activity.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ask for a FREE 10 µg sample of CPTII (G-5): sc-377294
Asked by: Zhenning
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05
I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with CPTII (G-5): sc-377294?
Asked by: Dr Ninau Qelp
We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here:
Rated 5 out of
Clean and specificWorked well for WB (mouse heart, one band at expected size). Originally ordered nonconjugated antibody which could not be delivered until Monday, however, we'd have a lockdown on Friday due to Covid19. Contacted customer service on Thursday afternoon, they were able to get me the HRP conjugated antibody delivered by Friday 1pm, and I got the result right before lockdown. Very happy about the results, perfect for my paper revision. Thank you!
Date published: 2020-03-21
Rated 5 out of
Good for mouse tissuegave a strong and clean band of the right size from mouse tissue
Date published: 2018-12-04
Rated 5 out of
Good clear bands in WBAntibody (1:500) showed one clear band in WB of primary mouse hepatocyte protein lysates
Date published: 2018-01-10
Rated 5 out of
Produced positive Western blot data of CPTIIProduced positive Western blot data of CPTII expression in Hep G2 whole cell lysate. -SCBT QC