Date published: 2025-9-8
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PARP1 Antibody (194C1439) - Western Blotting - Image 17083
PARP-1 항체 (194C1439): sc-56196. IMR-32 (A), Jurkat (B) 및 HeLa (C) 전체 세포 용해물에서의 PARP-1 발현 서양 블롯 분석.
PARP-1 항체 (194C1439): sc-56196. IMR-32 (A), Jurkat (B) 및 HeLa (C) 전체 세포 용해물에서의 PARP-1 발현 서양 블롯 분석.

PARP1 항체 (194C1439): sc-56196

4.3(26)
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데이터 시트
  • PARP1 항체 194C1439 는 마우스 monoclonal IgG2b PARP1 항체, 131간행물에 인용, 이며 50 µg/ 0.5 ml으로 제공합니다.
  • human PARP-1의 C-terminal 절단 부위 근처에 있는 에피토프 매핑을 가진 합성 펩타이드에 대해 생성됨
  • WB와 IP으로 mouse, rat와 human origin의 cleavad product of PARP-1을 검출할것을 권장합니다.
  • PARP1 (194C1439): sc-56196의 무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • 2b BP-HRP">m-IgG2b BP-HRP 는 PARP1 항체 (194C1439) WB 애플리케이션용입니다. 이 시약은 이제 PARP1 항체 (194C1439)와 함께 번들로 제공됩니다(아래 주문 정보 참조).

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추가 PARP 항체 pADPr, PARP-1, PARP-10, PARP-14, PARP-2, PARP-3 및 PARP-4

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

PARP1 항체 (194C1439)

sc-56196
50 µg/ 0.5 ml
$316.00

PARP1 (194C1439): m-IgG2b BP-HRP 번들

sc-548435
50 µg Ab; 10 µg BP
$354.00

该抗体在K562中是否能检测总parp？

Asked by: Evelina
感谢您的提问。您可以拨打电话：021-60936350，发送邮件到asia@scbio.cn，或者我们官网上的在线聊天，联系我们的亚洲技术支持团队，了解更多信息。 Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Service SH
Date published: 2022-12-16

Can you recommend a positive control to use with sc-56196:cleaved PARP-1 (194C1439) for Western blot?

Asked by: jenni
We recommend HeLa: sc-2200, Jurkat: sc-2204 or IMR-32: sc-2409 whole cell lysates as we have great Western blot data showing specific detection of cleaved PARP-1 in these cell lines.
Answered by: Technical Support 13
Date published: 2017-01-04
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Rated 4 out of 5 by from Anti-cleaved PARP1 per analisi in western blotL'Ab è stato testato utilizzando su lisati cellulari di linee cellulari di tumore mammario ottenuti dopo lisi RIPA buffer. 30 ug di proteine sono state separate tramite SDS-Page e trasferite su un filtro di nitrocellulosa. Il bloccaggio è stato effettuato in Blotto A, l'Ab primario è stato utilizzato alla diluizione di 1:1000 in 10 mL di Blotto A. Il secondario 1:2000 in Blotto A. Il segnale ottenuto è pulito, con un'unica banda del perso molecolare atteso.
Date published: 2025-01-15
Rated 1 out of 5 by from Extremely weak signalThis antibody gives a signal corresponding to cleaved PARP-1 (observed molecular weight: 89 kDa) but, unfortunately, this signal is extremely weak and sometimes not perceptible, even by using positive controls (staurosporine-treated cells) and high resolution ECL. We tested it at several working dilutions (ranging from 1:1.000 to 1:200) and we always obtained similar results. Notably, cleaved PARP1 was clearly visible in the same experimental conditions (40 ug of protein lysates from murine N1E-115 and human SH-SY5Y) by using another commercial antibody.
Date published: 2021-12-12
Rated 1 out of 5 by from Awful. inespecificWe bought this antibody two weeks ago, for this experiment, but clearly we have waste the money
Date published: 2021-03-30
Rated 4 out of 5 by from Good antibody for human melanoma cellsThe antibody diluited 1:200 worked well in human melanoma cells
Date published: 2020-02-03
Rated 5 out of 5 by from wonderful antibody!1:200 diluted for wb of rat protein,the band is quite strong and special
Date published: 2018-05-15
Rated 5 out of 5 by from mouse heart tissue extracts.The antibody worked really well in the mouse heart tissue extracts.
Date published: 2018-03-08
Rated 4 out of 5 by from takes some effort12% gel; K562 whole cell lysate; Western Blot 1:200, 4 degrees C overnight, high resolution ECL. First lane: untreated K562, 27ug; second lane: staurosporine treated K562, 20ug; third lane: staurosporine treated K562, 27ug.
Date published: 2018-01-08
Rated 4 out of 5 by from Bonne detectionJ'ai testé un échantillon de cet anticorps en WB et je suis satisfait du résultat.
Date published: 2017-11-28
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PARP1 항체 (194C1439) is rated 4.3 out of 5 by 26.
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