안티-cathepsin H 항체 F-7 는 마우스 monoclonal IgG1 (kappa light chain) cathepsin H 항체 이며 200 µg/ml으로 제공합니다.
human origin의 cathepsin H에서 내부에 위치한 151-280 아미노산을 항원으로 사용하였습니다.
cathepsin H 항체 (F-7)는 WB, IP, IF and ELISA으로 mouse, rat, human유래의 cathepsin H 를 감지하는 데에 추천한다 .
IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-cathepsin H 항체 (F-7)를 제공한다.
WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
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cathepsin H Antibody (F-7) is a high quality monoclonal cathepsin H antibody (also designated CTSH antibody) suitable for the detection of the cathepsin H protein of mouse, rat and human origin. cathepsin H Antibody (F-7) is available as both the non-conjugated anti-cathepsin H antibody form, as well as multiple conjugated forms of anti-cathepsin H antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Cathepsin H (also designated N-benzoylarginine-beta-naphthylamide hydrolase, aleurain, cathepsin B3 or cathepsin BA) is a lysosomal cysteine proteinase that mediates degradation of lysosomal proteins. Cathepsin H is a disulfide-linked heavy and light chain dimer produced from a single precursor protein. The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Elevated levels of cathepsin H correlates with malignant progression of prostate tumors. Two transcript variants encoding different isoforms have been found for this gene. Full-length and truncated cathepsin H [12-amino acid deletion in the signal peptide region (CTSHDelta10-21)] are expressed in prostate tissues, LNCaP, PC-3 and DU-145 prostate cancer cell lines. Cathepsin H mediates maturation of the biologically active surfactant protein-B (SP-B) peptide.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What is the recommended fixation method for immunofluorescence staining with cathepsin H (F-7): sc-398527 monoclonal antibody?
Asked by: TinTin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: