CaMKIδ Antibody (C-9): sc-374028
- CaMKIδ Antibody (C-9) is a mouse monoclonal IgG1 κ CaMKIδ antibody provided at 200 µg/ml
- raised against amino acids 319-385 mapping at the C-terminus of CaMKIδ of human origin
- CaMKI delta Antibody (C-9) is recommended for detection of CaMKIδ of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-CaMKI delta Antibody (C-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CaMKIδ Antibody (C-9) for WB applications. These reagents are now offered in bundles with CaMKIδ Antibody (C-9) (see ordering information below).
CaMKIδ Antibody (C-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects CaMKI delta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CaMKIδ (C-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CaMKI delta protein, also known as Calcium/calmodulin-dependent protein kinase type 1δ, is a 385 amino acid serine/threonine kinase that plays a crucial role in calcium-mediated signaling pathways. CaMKI delta is primarily located in both the nucleus and cytoplasm, which is significant as this dual localization allows participation in diverse cellular processes, including regulation of gene expression and cytoskeletal dynamics. CaMKI delta is activated by calcium-bound calmodulin, which induces a conformational change that enables phosphorylation by CaMKKα and CaMKKβ, ultimately leading to activation. This activation is vital for phosphorylation of target proteins, thereby influencing granulocyte function and other calcium-dependent cellular responses. Notably, variations in the gene encoding CaMKI delta have been associated with an increased risk of type 2 diabetes, highlighting importance in metabolic regulation. Additionally, two isoforms of CaMKI delta arise from alternative splicing, further diversifying functional roles in cellular signaling.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CaMKIδ Antibody (C-9) References:
- Identification and characterization of CKLiK, a novel granulocyte Ca(++)/calmodulin-dependent kinase. | Verploegen, S., et al. 2000. Blood. 96: 3215-23. PMID: 11050006
- Ca(2+)/CaM-dependent kinases: from activation to function. | Hook, SS. and Means, AR. 2001. Annu Rev Pharmacol Toxicol. 41: 471-505. PMID: 11264466
- Identification and characterization of novel components of a Ca2+/calmodulin-dependent protein kinase cascade in HeLa cells. | Ishikawa, Y., et al. 2003. FEBS Lett. 550: 57-63. PMID: 12935886
- Characterization of the role of CaMKI-like kinase (CKLiK) in human granulocyte function. | Verploegen, S., et al. 2005. Blood. 106: 1076-83. PMID: 15840691
- Meta-analysis of genome-wide association data and large-scale replication identifies additional susceptibility loci for type 2 diabetes. | Zeggini, E., et al. 2008. Nat Genet. 40: 638-45. PMID: 18372903
- Association testing of novel type 2 diabetes risk alleles in the JAZF1, CDC123/CAMK1D, TSPAN8, THADA, ADAMTS9, and NOTCH2 loci with insulin release, insulin sensitivity, and obesity in a population-based sample of 4,516 glucose-tolerant middle-aged Danes. | Grarup, N., et al. 2008. Diabetes. 57: 2534-40. PMID: 18567820
- Novel meta-analysis-derived type 2 diabetes risk loci do not determine prediabetic phenotypes. | Staiger, H., et al. 2008. PLoS One. 3: e3019. PMID: 18714373
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CaMKIδ Antibody (C-9) | sc-374028 | 200 µg/ml | $322.00 | |||
CaMKIδ Antibody (C-9): m-IgG Fc BP-HRP Bundle | sc-527429 | 200 µg Ab; 10 µg BP | $361.00 | |||
CaMKIδ Antibody (C-9): m-IgG1 BP-HRP Bundle | sc-532802 | 200 µg Ab; 20 µg BP | $361.00 | |||
CaMKIδ Antibody (C-9) AC | sc-374028 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CaMKIδ Antibody (C-9) HRP | sc-374028 HRP | 200 µg/ml | $322.00 | |||
CaMKIδ Antibody (C-9) FITC | sc-374028 FITC | 200 µg/ml | $336.00 | |||
CaMKIδ Antibody (C-9) PE | sc-374028 PE | 200 µg/ml | $349.00 | |||
CaMKIδ Antibody (C-9) Alexa Fluor® 488 | sc-374028 AF488 | 200 µg/ml | $364.00 | |||
CaMKIδ Antibody (C-9) Alexa Fluor® 546 | sc-374028 AF546 | 200 µg/ml | $364.00 | |||
CaMKIδ Antibody (C-9) Alexa Fluor® 594 | sc-374028 AF594 | 200 µg/ml | $364.00 | |||
CaMKIδ Antibody (C-9) Alexa Fluor® 647 | sc-374028 AF647 | 200 µg/ml | $364.00 | |||
CaMKIδ Antibody (C-9) Alexa Fluor® 680 | sc-374028 AF680 | 200 µg/ml | $364.00 | |||
CaMKIδ Antibody (C-9) Alexa Fluor® 790 | sc-374028 AF790 | 200 µg/ml | $364.00 |