Date published: 2025-9-8
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c-Fos Antibody (C-10) - Western Blotting - Image 379667
c-Fos 항체(C-10): sc-271243. A-431(A), Y79(B), SK-BR-3(C), C2C12(D) 및 TK-1(E) 전체 세포 용해물에서 c-Fos 발현의 웨스턴 블롯 분석.
c-Fos 항체(C-10): sc-271243. A-431(A), Y79(B), SK-BR-3(C), C2C12(D) 및 TK-1(E) 전체 세포 용해물에서 c-Fos 발현의 웨스턴 블롯 분석.

c-Fos 항체 (C-10): sc-271243

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데이터 시트
  • c-Fos 항체(C-10) 는 마우스 monoclonal IgG2aκ, 71간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human origin의 c-Fos에서 에 가까운120-155의 아미노산사이에 위치한 항원결정부와 특정결합합니다.
  • c-Fos 항체 (C-10)는 WB, IP, IF, IHC(P) and ELISA으로mouse, rat, human and 유래의 c-Fos, Fos B, Fra-1 and Fra-2 를 감지하는 데에 추천한다 ; 이외에, equine, canine, bovine, porcine and avian등 species와 반응할수 있습니다
  • ChIP 에 사용하는 TransCruz시약(sc-271243 X, 200 µg/0.1 ml)이 있습니다.
  • 항-c-Fos 항체(C-10)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • c-Fos (C-10): sc-271243의 무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP 및 2a BP-HRP">m-IgG2a BP-HRP 는 c-Fos 항체 (C-10) WB 및 IHC(P) 애플리케이션용입니다. 이 시약은 이제 c-Fos 항체 (C-10)와 함께 번들로 제공됩니다(아래 주문 정보 참조).

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다음을 포함한 추가 c-Fos 항체 Fos B

c-Fos 항체(C-10)는 마우스, 쥐 및 인간 유래의 c-Fos, Fos B, Fra-1 및 Fra-2를 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgG2a κ 마우스 모노클로날 c-Fos 항체입니다. c-Fos 항체(C-10)는 비공액형 항-c-Fos 항체 형태뿐만 아니라 아가로스, HRP, PE, FITC 및 여러 Alexa Fluor® 접합체를 포함한 여러 공액형 항-c-Fos 항체 형태로도 제공됩니다. c-Fos 종양 유전자는 처음에 두 개의 독립적인 쥐 골육종 바이러스 분리체와 조류 신세포종 바이러스에서 검출되었습니다. 세포 상동체인 c-Fos는 다양한 약제에 의해 신속하고 일시적으로 유도되는 핵 인산화 단백질을 암호화하며 여러 유전자의 전사 조절자로서 기능합니다. 특정 DNA 반응 요소에 결합하는 동이합체 및 이합체를 형성하는 c-Jun 단백질과 달리, c-Fos 단백질은 Jun 유전자 계열의 구성원들과만 이합체로 활성화됩니다. c-Fos의 기능적 상동체에는 Fra-1, Fra-2 및 Fos B 유전자가 포함됩니다. 또한, 선택된 ATF/CREB 패밀리 멤버는 Fos 및 Jun과 류신 지퍼 이량체를 형성할 수 있습니다. 서로 다른 이합체는 AP-1 및 CRE 부위에 대한 차별적인 특이성과 친화성을 나타냅니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

c-Fos 항체 (C-10)

sc-271243
200 µg/ml
$316.00

c-Fos (C-10): m-IgG Fc BP-HRP 번들

sc-525793
200 µg Ab; 10 µg BP
$354.00

c-Fos (C-10): m-IgG2a BP-HRP 번들

sc-546417
200 µg Ab; 10 µg BP
$354.00

c-Fos 항체 (C-10) X

sc-271243 X
200 µg/0.1 ml
$316.00

c-Fos 항체 (C-10) AC

sc-271243 AC
500 µg/ml, 25% agarose
$416.00

c-Fos 항체 (C-10) HRP

sc-271243 HRP
200 µg/ml
$316.00

c-Fos 항체 (C-10) FITC

sc-271243 FITC
200 µg/ml
$330.00

c-Fos 항체 (C-10) PE

sc-271243 PE
200 µg/ml
$343.00

c-Fos 항체 (C-10) Alexa Fluor® 488

sc-271243 AF488
200 µg/ml
$357.00

c-Fos 항체 (C-10) Alexa Fluor® 546

sc-271243 AF546
200 µg/ml
$357.00

c-Fos 항체 (C-10) Alexa Fluor® 594

sc-271243 AF594
200 µg/ml
$357.00

c-Fos 항체 (C-10) Alexa Fluor® 647

sc-271243 AF647
200 µg/ml
$357.00

c-Fos 항체 (C-10) Alexa Fluor® 680

sc-271243 AF680
200 µg/ml
$357.00

c-Fos 항체 (C-10) Alexa Fluor® 790

sc-271243 AF790
200 µg/ml
$357.00

c-Fos (C-10) 중화펩타이드

sc-271243 P
100 µg/0.5 ml
$68.00

Because there is no way to know if it works on mouse tissue until we try. To avoid anti-mouse secondary, it would be nice to try anti-Fos conjugated to Alexa 488 or 594. Do you have small samples of these?

Asked by: Svet
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

How can I get a free sample? I want to test it before making purchases. Thanks!

Asked by: kisong
Thank you for your question. Please contact Technical Service or your local distributor for information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-04-29

Is there a mouse-on-mouse issue, when used on mouse tissue?

Asked by: Berry2019
Thank you for your question. There are numerous publications citing use of this mouse monoclonal c-Fos antibody on mouse tissue. Typically, unless your tissues are rich with endogenous IgG there should not be a lot of non-specific binding using a mouse monoclonal antibody on mouse tissue. However, we do offer this antibody directly conjugated, so you can avoid having to use any anti-mouse IgG secondary antibody for detection, and have the most specific result.
Answered by: Technical Support
Date published: 2019-03-15

Hello, I would like to know the price of this product (Is c-Fos (C-10): sc-271243 monoclonal antibody). Thank you!

Asked by: Aline F Melo
Thank you for your question. Please visit our website for up-to-date pricing information: https://www.scbt.com/scbt/product/c-fos-antibody-c-10 ?requestFrom=search
Answered by: Technical Support
Date published: 2019-01-06

What is the expiration date of this ab kept at 4C?

Asked by: nf85
All of our products are warrantied for one year from the date purchased. We do not provide expiration dates for our antibodies. If properly stored at 4C it can continue to be viable for several years if not longer.
Answered by: Technical Support
Date published: 2018-12-21

这个抗体已经买了一年半了，现在还能用吗？

Asked by: wwwwwwwww
感谢您的提问。抱歉这个没法确认，您可以试验试试。更多问题，欢迎通过：电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”，联系我们的技术支持部门。 Thank you for your inquiry. Sorry that we can not sure, maybe you can do a test. For more questions, please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 15
Date published: 2017-11-01

Is c-Fos (C-10): sc-271243 monoclonal antibody appropriate for use in IHC with paraffin-embedded sections?

Asked by: jenniferc
Thank you for your question. Yes, c-Fos (C-10): sc-271243 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 10
Date published: 2017-01-10
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Rated 5 out of 5 by from Works correctly.We have tested the antibody for IF at a 1/500 concentration and Alexa secondary antibodies at 1/500. In our hands, it works correctly. Images of mice injected with a hypertonic saline solution intraperitoneally.
Date published: 2025-03-05
Rated 4 out of 5 by from mouse monoclonal anti-c-Fosmouse monoclonal anti-c-Fos (sc-271243) Santa Cruz Biotechnology; diluted 1:1000
Date published: 2021-05-11
Rated 4 out of 5 by from Worked for mouse brain tissueI tried c-Fos ab C-10, D-1, E-8 at the same time in PFA-fixed flozen mouse brain and found only C-10 worked well. I applied 1:500 ~ 1:8000 dilution in 4°C, over night for floated 40um thick-sections. 1:8000 dilution was better for me.
Date published: 2021-02-18
Rated 1 out of 5 by from It is not useful for immunohistochemitryWe processed sections of the same subject with different dilutions and with a polyclonal antibody that has resulted very good for our purposes. The monoclonal c-Fos (C-10) did not give a good immunostaining. Moreover, it uncovered structures that look like blood vessels and obstruct a good appreciation of the c-Fos positive cells. It is important to indicate that such vessels were not vissible with our reference antibody.
Date published: 2018-04-27
Rated 5 out of 5 by from Worked great for IFI was looking for a robust c-Fos antibody for immuno-staining in mouse brain tissue. This one worked like a champ. I used 1:300 in PBST, RT o.n. for 100um slice. For secondary, I used Invitrogen isotype specific Alexa 647 A-21241 1:300 in PBST, RT o.n. and other secondary abs yielded nonspecific signals.
Date published: 2018-03-21
Rated 5 out of 5 by from Best SCBT alternative to sc-52We tried every alternative and this is by far the best. Great staining at 1:500.
Date published: 2018-03-14
Rated 5 out of 5 by from excellent almost same as sc-52it worked great on brain sections at 1:500 dilution, I feel better than the previous rabbit poly clonal
Date published: 2017-05-18
Rated 5 out of 5 by from goodsIts specificity for epitope mapping between amino acids 120-155 in the internal region of c-Fos of human origin is very clear and very useful
Date published: 2017-01-17
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c-Fos 항체 (C-10) is rated 4.3 out of 5 by 14.
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