BCKDK Antibody (E-12): sc-374425

BCKDK Antibody (E-12) is a mouse monoclonal IgG1 antibody that detects BCKDK in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). BCKDK, or branched chain ketoacid dehydrogenase kinase, is a 412 amino acid mitochondrial matrix protein that functions as a monomer and features a histidine kinase domain, which is crucial for enzymatic activity. BCKDK is ubiquitously expressed and plays a vital role in regulating the branched-chain alpha-ketoacid dehydrogenase (BCKD) complex, a key regulatory enzyme in the catabolism of branched-chain amino acids (BCAAs) such as valine, leucine, and isoleucine. The BCKD complex catalyzes the irreversible oxidative decarboxylation of BCAAs, making BCKD essential for energy production and metabolic homeostasis. By phosphorylating and inactivating the BCKD complex, BCKDK serves as a critical regulator of BCAA metabolism, influencing various physiological processes, including energy balance and muscle metabolism. BCKDK′s ability to modulate BCKD complex activity underscores BCKDK′s importance in metabolic pathways and highlights BCKDK′s potential as a target for therapeutic interventions in metabolic disorders.

The preferred BCKDK antibody is BCKDK Antibody (F-10): sc-374424.