human origin의 ATP6E에서 에 가까운158-184의 아미노산사이에 위치한 항원결정부와 특정결합합니다.
안티-ATP6E 항체 (G-3)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 ATP6E 를 감지하는 데에 추천한다.
IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-ATP6E 항체 (G-3)를 제공한다.
WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for ATP6E Antibody (G-3) for WB applications. This reagent is now offered in a bundle with ATP6E Antibody (G-3) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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ATP6E Antibody (G-3) is a high quality monoclonal ATP6E antibody (also designated ATP6E antibody) suitable for the detection of the ATP6E protein of mouse, rat and human origin. ATP6E Antibody (G-3) is available as both the non-conjugated anti-ATP6E antibody form, as well as multiple conjugated forms of anti-ATP6E antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. ATP6E, also known as V-ATPase E, is a vacuolar-type H+-ATPase (V-ATPase). V-ATPase is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and an integral V0 domain, which is responsible for proton translocation, compose V-ATPase. Nine subunits (A-H) make up the V1 domain and five subunits (a, d, c, c′ and c") make up the V0 domain. Like F-ATPase, V-ATPase most likely operates through a rotary mechanism. ATP6E controls acidification of the vacuolar system and provides the main proton-motive force.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What application is the blocking peptide sc-514143 P appropriate for?
Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here:
Rated 5 out of
Great Western blot data of ATP6E expressionGreat Western blot data of ATP6E expression in MOLT-4, K-562, HEK293 and MCF7 whole cell lysates and human spleen tissue extract . -SCBT QC