human origin의 APC8에서 N-terminus에 위치한 1-300 아미노산을 항원으로 사용하였습니다.
안티-APC8 항체 (D-7)는 WB, IP, IF and ELISA으로 mouse, rat and human유래의 APC8 를 감지하는 데에 추천한다.
IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-APC8 항체 (D-7)를 제공한다.
WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) is the preferred secondary detection reagent for APC8 Antibody (D-7) for WB applications. This reagent is now offered in a bundle with APC8 Antibody (D-7) (see ordering information below).
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APC8 Antibody (D-7) is a high quality monoclonal APC8 antibody (also designated APC8 antibody) suitable for the detection of the APC8 protein of mouse, rat and human origin. APC8 Antibody (D-7) is available as both the non-conjugated anti-APC8 antibody form, as well as multiple conjugated forms of anti-APC8 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Composed of more than ten subunits, the anaphase-promoting complex (APC) acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis. APC, or cyclosome, accomplishes this progression through the ubiquitination of mitotic cyclins and other regulatory proteins that are targeted for destruction during cell division. APC is phosphorylated, and thus activated, by protein kinases Cdk1/cyclin B and polo-like kinase (Plk). APC is under tight control by a number of regulatory factors, including CDC20, CDH1 and MAD2. Specifically, CDC20 and CDH1 directly bind to APC and activate the cyclin-ubiquitination activity of APCs. In contrast, MAD2 inhibits APC by forming a ternary complex with CDC20 and APC and thus preventing APC activation. APC8, also referred to as CDC23, contains nine tetratricopeptide repeat (TPR) units. The TPR is a 34 amino acid sequence that is common to a variety of proteins and is significant because it forms scaffolds to mediate protein-protein interactions. The APC8 gene maps to human chromosome 5q31.2, within the smallest commonly deleted segment in myeloid leukemias.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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What is the recommended fixation method for immunofluorescence staining with APC8 (D-7): sc-514006 monoclonal antibody?
Asked by: Professor Griffin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: