Annexin VIII 항체 (D-1) WB, IP, IF, IHC(P) 와ELISA으로 human origin의 Annexin VIII을 검출할것을 권장합니다.

Annexin VIII 항체 (D-1): sc-271577

Annexin VIII 항체 (D-1) is rated 5.0 out of 5 by 4.
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데이터 시트

    • Annexin VIII 항체(D-1) 는 마우스 monoclonal IgG2a(kappa light chain) 이며 200 µg/ml으로 제공합니다.
    • raised against amino acids 121-180 mapping within an internal region of Annexin VIII of human origin
    • WB, IP, IF, IHC(P) 와ELISA으로 human origin의 Annexin VIII을 검출할것을 권장합니다.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Annexin VIII Antibody (D-1) for WB and IHC(P) applications. These reagents are now offered in bundles with Annexin VIII Antibody (D-1) (see ordering information below).
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I am using Annexin VIII (D-1): sc-271577 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Positive detection of endogenous Annexin Positive detection of endogenous Annexin VIII by WB using JAR whole cell lysate. -SCBT QC
Date published: 2015-05-01
Rated 5 out of 5 by from Good specificity shown by using transfected Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the human Annexin VIII protein via Western Blot analysis. -SCBT QC
Date published: 2015-03-18
Rated 5 out of 5 by from Distinct band observed corresponding to the Distinct band observed corresponding to the Annexin VIII protein's molecular weight by WB using HeLa whole cell lysate. -SCBT QC
Date published: 2015-02-27
Rated 5 out of 5 by from Superb immunoperoxidase cytoplasmic and nuclear Superb immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human urinary bladder tissue. -SCBT QC
Date published: 2015-02-12
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