AMPK beta 1 항체 (Z14) WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 AMPKβ1을 검출할것을 권장합니다.

AMPKβ1 항체 (Z14): sc-100357

AMPKβ1 항체 (Z14) is rated 4.9 out of 5 by 7.
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Datasheets

    • 안티-AMPK beta 1 항체 Z14 는 마우스 monoclonal IgG2a (kappa light chain) AMPKβ1 항체 이며 100 µg/ml으로 제공합니다.
    • human origin의 AMPKβ1의 전체인 1-271 아미노산을 항원으로 사용하였습니다.
    • WB, IP, IF와 ELISA으로 mouse, rat와 human origin의 AMPKβ1을 검출할것을 권장합니다.
    • 강력추천하는 conventional secondary anti-mouse IgG reagents의 대체제품 보기m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP).
    • AMPKβ1 (Z14): sc-100357무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.

I am using AMPKβ1 (Z14): sc-100357 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Cweed
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Good Antibody for WB We can get clear band. Application: WB Sample: rat microglia culture
Date published: 2020-08-11
Rated 5 out of 5 by from good product This antibody works pretty good for WB experiments.
Date published: 2018-09-11
Rated 4 out of 5 by from WB AMPK β1 (Z-14) One unique strong and clear band at 38 kDa. 40 ug of whole cell extract from 12 month old murine hepatocellular carcinoma (2) tumoral tissue (1) adjacent non tumoral tissue. Dilution 1/1000.
Date published: 2017-10-13
Rated 5 out of 5 by from Great features I applied a sample one month ago and I did Westen Blotting (dilution 1:100) at white adipose tissue of mouse, the results is excellent.
Date published: 2017-09-20
Rated 5 out of 5 by from Strong band in WB in HeLa whole cell lysate Strong band in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-05-03
Rated 5 out of 5 by from Clear band in WB in lambda protein phosphatase Clear band in WB in lambda protein phosphatase treated differentiated C2C12 whole cell lysate. -SCBT QC
Date published: 2015-04-25
Rated 5 out of 5 by from Produced excellent immunofluorescence nuclear Produced excellent immunofluorescence nuclear and cytoplasmic staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-01-25
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