aggrecan 항체 (4F4) WB, IP, IF 와IHC(P)으로 human origin의 aggrecan을 검출할것을 권장합니다.

aggrecan 항체 (4F4): sc-33695

aggrecan 항체 (4F4) is rated 4.5 out of 5 by 13.
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데이터 시트

    • 안티-aggrecan 항체 4F4 는 마우스 monoclonal IgG1 κ aggrecan 항체, 32간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • raised against human articular cartilage aggrecan
    • aggrecan 항체 (4F4)는 WB, IP, IF and IHC(P)으로 human유래의 aggrecan 를 감지하는 데에 추천한다 .
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-aggrecan 항체 (4F4)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • aggrecan (4F4): sc-33695무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for aggrecan Antibody (4F4) for WB and IHC(P) applications. These reagents are now offered in bundles with aggrecan Antibody (4F4) (see ordering information below).
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Hi there! Has this antibody been used in brain/spinal cord tissue for IHC?

Asked by: shantan
Thank you for your question. We have many citations of this antibody in IF and IHC, but unfortunately I do not see if any of them are in brain/spinal cord. Aggrecan is detected in chondrocytes throughout the developing skeleton/ cartilage and we have many publications in bone marrow.
Answered by: Tech Service
Date published: 2019-11-19

Can aggrecan (4F4) sc-33695 monoclonal antibody be used with porcine tissue or cells?

Asked by: Vanessa_silos
Thank you for your question. We have not tested this antibody in porcine tissue. We are aware of one product citation using this antibody in porcine tissue. Please refer to the following publication for details and verification, PMID: 19112334.
Answered by: Technical Support
Date published: 2019-04-13

I would like to detection of aggrecan in western blot assay. I tried dilution 1:500 in 5% skinmilk. and 2nd antibody used sc-2005 product. but It does not detection. How can I do? my sample orign is human pellet.

Asked by: vtml
Thank you for your question. We recommend starting with a 1:100 primary antibody dilution for Western blot. For further assistance, please contact Technical Service.
Answered by: Technical Support
Date published: 2018-12-06

Can aggrecan (4F4): sc-33695 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, aggrecan (4F4): sc-33695 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2019-02-28
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Rated 4 out of 5 by from Good, it can be used in IHC I used in bone in 1:100. it's specific. I think it can used in 1:200.
Date published: 2017-08-09
Rated 4 out of 5 by from Good antibody in detecting aggrecan This antibody works very well in detecting cell cycle protein aggrecan without any nonspecific bands.
Date published: 2017-06-01
Rated 5 out of 5 by from Great antibody. Antibody provides clear and specific results.Great antibody.
Date published: 2017-02-13
Rated 5 out of 5 by from Excellent aggrecan antibody antibody is really great. we got specific signal for the protein at the right molecular weight.
Date published: 2017-02-08
Rated 5 out of 5 by from The use of this antibody was satisfactory The use of this antibody has a good specificity for WB dilution and the 1:500 results are perfect.
Date published: 2017-02-05
Rated 5 out of 5 by from Good, versatile antibody The aggrecan Antibody (4F4): sc-33695 works well for multiple applications including WB, IP, IF and IHC(P). This antibody has also been cited several times for further verification.
Date published: 2017-01-20
Rated 5 out of 5 by from Performance good antibody This antibody in the use of performance is very good, it produces a good effect in mice.
Date published: 2016-12-21
Rated 5 out of 5 by from Fantastic antibody of Agg! I have used many kinds of Agg from different companies, but unfortunately, some of them are not work well... But this Agg can easily get strong band at 1:1000! Beyond my imagination!
Date published: 2016-12-19
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