Nicotinic Acetylcholine Receptor alpha 8/CHRNA8 Antibody (308): sc-58609
- Nicotinic Acetylcholine Receptor alpha 8/CHRNA8 Antibody (308) is a rat monoclonal IgG2b provided at 200 µg/ml
- raised against bacterially expressed large cytoplasmic domain of AChRα8 of chicken origin
- Nicotinic Acetylcholine Receptor alpha 8/CHRNA8 Antibody (308) is recommended for detection of the cytoplasmic surface of both native and denatured nicotinic AChRα8 between amino acids 323-342 of avian origin by WB, IP and IF
- Anti-Nicotinic Acetylcholine Receptor alpha 8/CHRNA8 Antibody (308) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Nicotinic Acetylcholine Receptor alpha 8/CHRNA8 Antibody (308). This work is in progress.
AChRα8 Antibody (308) is a rat monoclonal IgG2b antibody that detects nicotinic acetylcholine receptor alpha 8 (AChRα8) in avian species through western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-AChRα8 antibody (308) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AChRα8 is a crucial component of the nicotinic acetylcholine receptor family, playing a vital role in fast synaptic transmission by forming a transmembrane aqueous pore that allows ions to flow in response to neurotransmitter binding. AChRα8 functions as a 456 amino acid multi-pass membrane protein predominantly located at the postsynaptic membrane of the neuromuscular junction, where AChRα8 binds acetylcholine and facilitates cation influx, leading to muscle contraction. Proper functioning of AChRα8 remains essential for neuromuscular signaling, and dysfunction can lead to significant neuromuscular disorders. AChRα8 undergoes various post-translational modifications, influencing activity and interactions with other proteins, further underscoring importance in cellular signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Nicotinic Acetylcholine Receptor alpha 8/CHRNA8 Antibody (308) References:
- Probing conformational changes in the nicotinic acetylcholine receptor by Fourier transform infrared difference spectroscopy. | Baenziger, JE., et al. 1992. Biophys J. 62: 64-6. PMID: 1600101
- Ligand-gated ion channels in the brain: the amino acid receptor superfamily. | Betz, H. 1990. Neuron. 5: 383-92. PMID: 1698394
- Acetylcholinesterase reactivators modify the functional properties of the nicotinic acetylcholine receptor ion channel. | Alkondon, M., et al. 1988. J Pharmacol Exp Ther. 245: 543-56. PMID: 2452874
- Neurotransmitter action: opening of ligand-gated ion channels. | Unwin, N. 1993. Cell. 72 Suppl: 31-41. PMID: 7679054
- The diversity of neuronal nicotinic acetylcholine receptors. | Sargent, PB. 1993. Annu Rev Neurosci. 16: 403-43. PMID: 7681637
- Quantal release of neurotransmitter and long-term potentiation. | Stevens, CF. 1993. Cell. 72 Suppl: 55-63. PMID: 8094037
- The role of NMDA receptors in information processing. | Daw, NW., et al. 1993. Annu Rev Neurosci. 16: 207-22. PMID: 8460891
- Functional contributions of alpha5 subunit to neuronal acetylcholine receptor channels. | Ramirez-Latorre, J., et al. 1996. Nature. 380: 347-51. PMID: 8598930
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nicotinic Acetylcholine Receptor alpha 8/CHRNA8 Antibody (308) | sc-58609 | 200 µg/ml | $322.00 | |||
Nicotinic Acetylcholine Receptor alpha 8/CHRNA8 Antibody (308) AC | sc-58609 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Nicotinic Acetylcholine Receptor alpha 8/CHRNA8 Antibody (308) HRP | sc-58609 HRP | 200 µg/ml | $322.00 | |||
Nicotinic Acetylcholine Receptor alpha 8/CHRNA8 Antibody (308) FITC | sc-58609 FITC | 200 µg/ml | $336.00 | |||
Nicotinic Acetylcholine Receptor alpha 8/CHRNA8 Antibody (308) PE | sc-58609 PE | 200 µg/ml | $349.00 | |||
Nicotinic Acetylcholine Receptor alpha 8/CHRNA8 Antibody (308) Alexa Fluor® 488 | sc-58609 AF488 | 200 µg/ml | $364.00 | |||
Nicotinic Acetylcholine Receptor alpha 8/CHRNA8 Antibody (308) Alexa Fluor® 546 | sc-58609 AF546 | 200 µg/ml | $364.00 | |||
Nicotinic Acetylcholine Receptor alpha 8/CHRNA8 Antibody (308) Alexa Fluor® 594 | sc-58609 AF594 | 200 µg/ml | $364.00 | |||
Nicotinic Acetylcholine Receptor alpha 8/CHRNA8 Antibody (308) Alexa Fluor® 647 | sc-58609 AF647 | 200 µg/ml | $364.00 | |||
Nicotinic Acetylcholine Receptor alpha 8/CHRNA8 Antibody (308) Alexa Fluor® 680 | sc-58609 AF680 | 200 µg/ml | $364.00 | |||
Nicotinic Acetylcholine Receptor alpha 8/CHRNA8 Antibody (308) Alexa Fluor® 790 | sc-58609 AF790 | 200 µg/ml | $364.00 |