AChE Antibody (A-11): sc-373901

AChE Antibody (A-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects acetylcholinesterase (AChE) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AChE monoclonal antibody (A-11) is raised against amino acids 481-614 of AChE of human origin. Anti-AChE antibody (A-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Acetylcholinesterase plays a crucial role in the nervous system by hydrolyzing acetylcholine at synaptic junctions, thereby terminating synaptic transmission and ensuring proper neuronal signaling. AChE exists in three forms due to alternative mRNA splicing: the T form, which is soluble and found in synapses; the H form, which is globular and located on the outer surfaces of cell membranes; and the R form, which is not functionally characterized. The H form′s subunits are glycosylphosphatidylinositol-anchored to cell membranes, while the T form′s subunits are tethered to basal lamina components via a collagen tail. Loss of AChE activity in cholinergic and noncholinergic neurons is associated with Alzheimer′s disease, where increased AChE activity is observed around amyloid plaques, potentially linked to disturbances in calcium homeostasis and the activation of L-type voltage-dependent calcium channels.

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AChE Antibody (A-11) References:

1. The role of acetylcholinesterase in the pathogenesis of Alzheimer's disease.  |  Rees, TM. and Brimijoin, S. 2003. Drugs Today (Barc). 39: 75-83. PMID: 12669110
2. H and T subunits of acetylcholinesterase from Torpedo, expressed in COS cells, generate all types of globular forms.  |  Duval, N., et al. 1992. J Cell Biol. 118: 641-53. PMID: 1639848
3. Termination and beyond: acetylcholinesterase as a modulator of synaptic transmission.  |  Zimmerman, G. and Soreq, H. 2006. Cell Tissue Res. 326: 655-69. PMID: 16802134
4. Bovine brain acetylcholinesterase primary sequence involved in intersubunit disulfide linkages.  |  Roberts, WL., et al. 1991. J Biol Chem. 266: 7481-7. PMID: 2019579
5. From protein to peptides: a spectrum of non-hydrolytic functions of acetylcholinesterase.  |  Halliday, AC. and Greenfield, SA. 2012. Protein Pept Lett. 19: 165-72. PMID: 21933122
6. Acetylcholinesterase inhibitors: pharmacology and toxicology.  |  Colović, MB., et al. 2013. Curr Neuropharmacol. 11: 315-35. PMID: 24179466
7. Acetylcholinesterase-Fc Fusion Protein (AChE-Fc): A Novel Potential Organophosphate Bioscavenger with Extended Plasma Half-Life.  |  Noy-Porat, T., et al. 2015. Bioconjug Chem. 26: 1753-8. PMID: 26121420
8. Acetylcholinesterase and human cancers.  |  Richbart, SD., et al. 2021. Adv Cancer Res. 152: 1-66. PMID: 34353435
9. Neural regulation of acetylcholinesterase mRNAs at mammalian neuromuscular synapses.  |  Michel, RN., et al. 1994. J Cell Biol. 127: 1061-9. PMID: 7962068
10. Cloning and expression of a rat acetylcholinesterase subunit: generation of multiple molecular forms and complementarity with a Torpedo collagenic subunit.  |  Legay, C., et al. 1993. J Neurochem. 60: 337-46. PMID: 8417155
11. Expression of a cDNA encoding the glycolipid-anchored form of rat acetylcholinesterase.  |  Legay, C., et al. 1993. FEBS Lett. 315: 163-6. PMID: 8417973
12. The amyloid beta-protein of Alzheimer's disease increases acetylcholinesterase expression by increasing intracellular calcium in embryonal carcinoma P19 cells.  |  Sberna, G., et al. 1997. J Neurochem. 69: 1177-84. PMID: 9282941

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AChE Antibody (A-11) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-AChE Antibody (A-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AChE Antibody (A-11) Specifications

Alternate Product Name: Acetylcholinesterase

Product Clone Name: A-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 481-614

Epitope Target: AChE

Epitope Species: human

Product Reactivity/Detection: AChE

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for AChE Antibody (A-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin