Ac-Histone H4 항체 (E-5) WB, IP, IF, IHC(P)와 ELISA으로 broad species 와 origin의 Ser 1, Lys 5, Lys 8 and Lys 12 acetylated Histone H4을 검출할것을 권장합니다.

Ac-Histone H4 항체 (E-5): sc-377520

Ac-Histone H4 항체 (E-5) is rated 4.9 out of 5 by 8.
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데이터 시트

    • 안티-Ac-Histone H4 항체 E-5 는 마우스 monoclonal IgG2a κ Ac-Histone H4 항체, 5간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • specific for an epitope mapping to a short peptide containing acetylated Serine 1 and Lysine 5, 8 and 12 of Histone H4 of human origin
    • Ac-Histone H4 항체 (E-5)는 WB, IP, IF, IHC(P) and ELISA으로broad species and 유래의 Ser 1, Lys 5, Lys 8 and Lys 12 acetylated Histone H4 를 감지하는 데에 추천한다 .; 이외에, equine, canine, bovine, porcine and avian등 species와 반응할수 있습니다
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-Ac-Histone H4 항체 (E-5)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Ac-Histone H4 (E-5): sc-377520무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Ac-Histone H4 Antibody (E-5) for WB and IHC(P) applications. These reagents are now offered in bundles with Ac-Histone H4 Antibody (E-5) (see ordering information below).
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Is this antibody suitable for conjugation with an NHS Ester dye?

Asked by: sc j
Thank you for your question. This antibody is suitable for labeling with NHS ester dyes. For custom conjugations, please email [email protected] or contact our European support office for assistance. You may reach them toll-free by phone at 00800 4573 8000, by email at [email protected], or contact them by live chat on our website at www.scbt.com. Alternatively, you can contact one of our authorized distributors in region, which can be found in the following link: https://www.scbt.com/scbt/resources/distributors
Answered by: Blake J
Date published: 2021-07-12

What application is the blocking peptide sc-377520 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good antibody It works very well for my Western blot with 1:200 duiltion
Date published: 2018-04-12
Rated 5 out of 5 by from very useful in WB I got this antibody(E-5) almost two weeks ago,I found it was very useful in my WB experiments in the ratio 1:750. In fact, I got perfect result only one chance.So excited!
Date published: 2017-12-19
Rated 5 out of 5 by from This antibody was confirmed to be useful! I used the WB method to detect Ac-Histone H4 in UMUC3 cell line. The antibody (1: 200) is easy to detect the expression of Ac-Histone H4. The background is very clean and the molecular weight is also accurate.
Date published: 2017-11-01
Rated 4 out of 5 by from good I used this antibody in the ratio 1:500 in hela cell line and I got a clear brand
Date published: 2017-08-08
Rated 5 out of 5 by from Great ab Strong bands in WB experiments in cells treated with histone deacetylase inhibitor.
Date published: 2017-03-10
Rated 5 out of 5 by from IF: Nice nuclear staining with NIH/3T3 IF: Nice nuclear staining with NIH/3T3. -SCBT QC
Date published: 2015-04-08
Rated 5 out of 5 by from Great antibody for Western Great antibody for Western, strong band detected in NIH/3T3 and Trichostatin A treated NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-03-02
Rated 5 out of 5 by from Works well for IF applications Works well for IF applications, positive in HepG2. -SCBT QC
Date published: 2014-03-28
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