ABC1 siRNA (h), shRNA 와 Lentiviral Particle Gene Silencers human ABC1 - 특정 siRNA, shRNA 플라스미드 및 렌티 바이러스 유전자 사일런서

ABC1 siRNA (h), shRNA 와 Lentiviral Particle Gene Silencers

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데이터 시트

    • Target species: human; for corresponding mouse product, see ABC1 siRNA and shRNA Plasmids (m)
    • 유전자침묵제품은 보통 3-5개의 특정타깃 19-25개의 뉴클레오티드로 구성되였습니다.
    • ABC1의 침묵결과를 단독으로 확인할수있는 요소나 plasmid를 요청할시 단독으로 판매합니다.
    • 적절한 control 항체: ABC1 항체 (AC10): sc-53482 를 Western blotting이나 immunofluorescence으로 ABC1의 발현정황을 확인할시 권장하는 control항체입니다.
    • si/shRNA Gene Silencers에 관한 상세정보를 원하시면 클릭하세요
    • 유전자침묵제품은 비영리적인용도나 연구에만 사용가능합니다 자세한 정보보기.

Gene 정보

SpeciesGene NameGene IDChromosome LocationIsoform (mRNA) Accession #Protein Accession #OMIM™ Number
HumanABCA1199q31.1XM_005251773, XM_005251776, XM_011518339, XM_011518340, XM_011518341, XM_011518342, XM_011518344, XM_017014378, XM_017014379, XM_017014380, XM_017014381, XM_017014382, NM_005502O95477604091

Does all sc lentivirus have the same promoters? The reason I ask is that I have seen others cited using sc lentivirus successfully in Thp-1 cells for LPL. I heard that promoter selection is crucial for Thp-1 cells.

Asked by: weng
Thank you for your question. Yes, our vector uses an H1 promoter for shRNA transcription.
Answered by: Technical Support
Date published: 2019-04-23

I read the reference cited here. Is the shRNA targeting the same sequence? I want to make a stable knockdown of ABCA1 in Thp-1 cells. Does your shRNA guarantee knockdown? Does the product come with a positive control for transfection efficiency?

Asked by: weng
Thank you for your question. Yes, the ABC1 shRNA plasmid (h): sc-41136-SH is using the same target sequence as the siRNA product that is referenced in the product citation on our website. For stable knockdown, you may want to consider using the shRNA Lentiviral Particles. Please contact our Technical Service Department for more information. We do sell separately a positive control for measuring transfection efficiency, copGFP Control Plasmid: sc-108083. https://www.scbt.com/scbt/browse/shrna-plasmid-support-products/_/N-lhyfam Santa Cruz does warranty these si/shRNA reagents to show observable knockdown at the protein level.  Santa Cruz implements computationally designed published algorithms which are demonstrated to give greater than 50% knockdown more than 90% of the time. If you purchase the reagents and are not able to achieve the expected results we encourage you to contact our technical support staff.  If we are unable to trouble-shoot the assay further then we are able to offer you either research credit or refund the purchase price of the reagents.
Answered by: Technical Support
Date published: 2019-04-23
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