WRN Double Nickase Plasmid (m): sc-423724-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- WRN Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- WRN Double Nickase Plasmid (m) and WRN Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Wrn. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WRN Double Nickase Plasmid (m) | sc-423724-NIC | 20 µg | $410.00 | |||
WRN Double Nickase Plasmid (m2) | sc-423724-NIC-2 | 20 µg | $410.00 |
Mouse Wrn encodes WRN, a RecQ family DNA helicase/exonuclease that preserves genome stability by coordinating DNA replication, recombination, and multiple DNA repair processes. WRN participates in responses to replication stress and DNA double-strand break repair, functioning in pathways that include homologous recombination, non-homologous end joining, and telomere maintenance. Disruption of WRN activity is linked to heightened chromosomal instability, altered cell-cycle checkpoint signaling, and premature cellular senescence phenotypes. These functions make Wrn a widely used model gene for studying mechanisms of aging-associated genome maintenance and DNA damage response circuitry.
WRN Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Wrn locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Wrn. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Wrn function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Wrn-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.