UBE2D Antibody (F-6): sc-166277

UBE2D Antibody (F-6) is a mouse monoclonal IgG1 antibody that detects UBE2D in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). UBE2D is a crucial ubiquitin-conjugating enzyme that plays a significant role in the ubiquitin-proteasome system, which is essential for maintaining cellular homeostasis by regulating protein degradation. UBE2D is primarily located in the cytoplasm, where UBE2D facilitates the transfer of ubiquitin to target proteins, marking them for degradation. Proper functioning of UBE2D is vital, as UBE2D ensures the removal of damaged or misfolded proteins, thereby preventing the accumulation of potentially toxic proteins that could disrupt cellular functions. Additionally, UBE2D interacts with various E3 ligases, such as SCF (Skp1-Cul1-F-box protein) complexes, which are responsible for recognizing specific substrates for ubiquitination. The dynamic interplay between UBE2D and these ligases underscores the importance of UBE2D in cellular signaling pathways, including those involved in immune responses and cell cycle regulation. UBE2D Antibody (F-6) serves as an invaluable tool for researchers studying the mechanisms of protein degradation and the implications of ubiquitination in various diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

UBE2D Antibody (F-6) References:

1. Cadmium toxicity is caused by accumulation of p53 through the down-regulation of Ube2d family genes in vitro and in vivo.  |  Tokumoto, M., et al. 2011. J Toxicol Sci. 36: 191-200. PMID: 21467746
2. The IDOL-UBE2D complex mediates sterol-dependent degradation of the LDL receptor.  |  Zhang, L., et al. 2011. Genes Dev. 25: 1262-74. PMID: 21685362
3. The UbL protein UBTD1 stably interacts with the UBE2D family of E2 ubiquitin conjugating enzymes.  |  Uhler, JP., et al. 2014. Biochem Biophys Res Commun. 443: 7-12. PMID: 24211586
4. Inorganic arsenic induces apoptosis through downregulation of Ube2d genes and p53 accumulation in rat proximal tubular cells.  |  Tokumoto, M., et al. 2013. J Toxicol Sci. 38: 815-20. PMID: 24212999
5. Systematic E2 screening reveals a UBE2D-RNF138-CtIP axis promoting DNA repair.  |  Schmidt, CK., et al. 2015. Nat Cell Biol. 17: 1458-1470. PMID: 26502057
6. Accumulation of p53 via down-regulation of UBE2D family genes is a critical pathway for cadmium-induced renal toxicity.  |  Lee, JY., et al. 2016. Sci Rep. 6: 21968. PMID: 26912277
7. NEDD8 nucleates a multivalent cullin-RING-UBE2D ubiquitin ligation assembly.  |  Baek, K., et al. 2020. Nature. 578: 461-466. PMID: 32051583
8. Design of linked-domain protein inhibitors of UBE2D as tools to study cellular ubiquitination.  |  Bukhari, Z., et al. 2024. bioRxiv.. PMID: 39282319
9. UBR-5 and UBE2D mediate timely exit from stem fate via destabilization of poly(A)-binding protein PABP-2 in cell state transition.  |  Calva Moreno, JF., et al. 2024. Proc Natl Acad Sci U S A. 121: e2407561121. PMID: 39405353
10. The ubiquitin-conjugating enzyme UBE2D maintains a youthful proteome and ensures protein quality control during aging by sustaining proteasome activity.  |  Hunt, LC., et al. 2025. PLoS Biol. 23: e3002998. PMID: 39879147

The preferred UBE2D antibody is UBE2D Antibody (C-6): sc-166278.