Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TrpRS CRISPR/Cas9 KO Plasmid (m): sc-423692
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TrpRS CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TrpRS genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TrpRS Antibody (C-7): sc-374401
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TrpRS CRISPR/Cas9 KO Plasmid (m) | sc-423692 | 20 µg | $397.00 |
Overview
Wars encodes tryptophanyl-tRNA synthetase (TrpRS), a cytosolic aminoacyl-tRNA synthetase that ligates L-tryptophan to its cognate tRNATrp, providing charged tRNA required for accurate mRNA translation. By controlling tRNA charging and supporting ribosomal elongation, TrpRS helps couple nutrient availability to protein synthesis and cellular stress-adaptation programs. Perturbation of aminoacyl-tRNA synthetase activity can influence proteostasis, integrated stress signaling, and translational fidelity, processes frequently implicated in inflammation, neurodegeneration, and oncogenic transformation. Mouse Wars is therefore a useful node for studying how altered translation capacity reshapes cell state and disease-relevant phenotypes in vitro and in vivo.
TrpRS CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Wars gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Wars together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Wars open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TrpRS protein expression.
This CRISPR knockout system enables efficient generation of Wars-deficient cell models for investigation of TrpRS signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Wars exon(s) critical for TrpRS function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Wars genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TrpRS CRISPR/Cas9 KO Plasmid (m) and TrpRS CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Wars locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TrpRS HDR Plasmid (m) and TrpRS HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Wars homology arms to support homology-directed repair at defined Wars target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.