Tris-Borate-EDTA buffer, 10X A concentrated solution ready to use for DNA and RNA polyacrylamide gel electrophoresis, "TBE buffer".

Tris-Borate-EDTA buffer, 10X

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アプリケーション: Tris-Borate-EDTA buffer, 10X is "TBE buffer", a concentrated solution ready to use for DNA and RNA polyacrylamide gel electrophoresis
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* Refer to Certificate of Analysis for lot specific data (including water content).
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Tris-Borate-EDTA (TBE) buffer, 10X is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Since borate in TBE buffer is a strong inhibitor for many enzymes, TAE buffer (Tris Acetate-EDTA buffer, 10X powder, sc-296647) is recommended when looking at enzymatic applications for the DNA sample.
Additional forms available:
Tris-Borate-EDTA Buffer, 10X Powder Pack (sc-296651)
Tris-Borate-EDTA buffer, 5X (sc-296652)


参考文献

1. Orbán, L., et al. 1991. Electrophoresis. 12: 233-240. PMID: 2070779
2. Wu, M. and Kusukawa, N. 1998. BioTechniques. 24: 676-678. PMID: 9564543
3. Stellwagen, N.C. 1998. Electrophoresis. 19: 1542-1547. PMID: 9719523
4. Stellwagen, N.C., et al. 2000. Anal. Biochem. 287: 167-175. PMID: 11078596
5. Ren, J., et al. 2002. Anal Sci. 18: 469-471. PMID: 11999525
6. Biyani, M., et al. 2003. Electrophoresis. 24: 628-633. PMID: 12601730
7. Chen, Z. and Burns, M.A. 2005. Electrophoresis. 26: 4718-4728. PMID: 16294296
8. Zhou, P., et al. 2005. J Chromatogr A. 1083: 173-178. PMID: 16078704

用法 :
Ready for use after dilution to working concentrations. Dilution of the TBE stock concentrates to a 1X TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5X or 10X stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel.
Formulation :
10X
Solubility :
soluble in water
pH :
~8.3
保存方法 :
Store at room temperature
試験・研究用以外には使用しないでください。 臨床及び体外診断には使用できません。

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Tris-Borate-EDTA buffer, 10X  参考文献

See how others have used Tris-Borate-EDTA buffer, 10X. Click on the entry to view the PubMed entry .

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PMID: # 26519197  Kartal, K. et al. 2015. BMC Cancer. 15: 824.

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