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TLR4 Antibody (25) - Western Blotting - Image 348898 — TLR4 抗体 (25): sc-293072. PANC-1 (A), SK-N-MC (B), EOC 20 (C), WEHI-231 (D), SP2/0 (E), LADMAC (F)の全細胞溶解液におけるTLR4発現のウェスタンブロット解析.

TLR4 抗体 (25): sc-293072. PANC-1 (A), SK-N-MC (B), EOC 20 (C), WEHI-231 (D), SP2/0 (E), LADMAC (F)の全細胞溶解液におけるTLR4発現のウェスタンブロット解析.

TLR4抗体(25): sc-293072

Name: TLR4抗体(25)
Brand: SCBT
SKU: sc-293072
Price: 316.0 USD
Availability: InStock

4.7(106)

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データシート

TLR4抗体 25はマウスモノクローナルIgG₁TLR4 抗体です。200 µg/mlで提供
mouse由来のTLR4のアミノ酸198-395に対応します
mouse, rat, human と canine 由来のTLR4 WB, IP, IF と IHC(P)での検出にはお勧めします
IP用のアガロース に共役での利用可能です。WB、IHC(P)とELISA用のHRP に共役での利用可能です。
TLR4 (25): sc-293072の無料の10 µgサンプルを受け取るには、ご連絡ください。
m-IgG Fc BP-HRPは、TLR4 Antibody (25) WBおよびIHC(P)アプリケーション用。用の二次検出試薬です。この試薬は現在、TLR4 Antibody (25) とセットで提供されています（下記の注文情報をご参照ください）。

参考文献をチェックします (697)

クイックリンク

サポート品

説明

Gene情報

データシートとプロトコル

研究情報

TLR4抗体(25) 参考文献:

Toll様受容体-4はリポ多糖誘導性シグナル伝達を媒介する。 | Chow, JC., et al. 1999. J Biol Chem. 274: 10689-92. PMID: 10196138
TLR6：拡大するToll様受容体ファミリーの新規メンバー。 | Takeuchi, O., et al. 1999. Gene. 231: 59-65. PMID: 10231569
ペプチドグリカンおよびリポテイコ酸が誘導する細胞活性化は, toll様受容体2によって媒介される。 | Schwandner, R., et al. 1999. J Biol Chem. 274: 17406-9. PMID: 10364168
トール様受容体を介した微生物リポ蛋白質による宿主防御機構。 | Brightbill, HD., et al. 1999. Science. 285: 732-6. PMID: 10426995
ショウジョウバエのTollとIL-1レセプター。 | Gay, NJ. and Keith, FJ. 1991. Nature. 351: 355-6. PMID: 1851964
ショウジョウバエのTollタンパク質のヒトホモログは, 適応免疫の活性化をシグナルする。 | Medzhitov, R., et al. 1997. Nature. 388: 394-7. PMID: 9237759
ショウジョウバエのTollに構造的に関連するヒト受容体ファミリー。 | Rock, FL., et al. 1998. Proc Natl Acad Sci U S A. 95: 588-93. PMID: 9435236
Toll様受容体-2はリポ多糖誘発細胞シグナル伝達を仲介する。 | Yang, RB., et al. 1998. Nature. 395: 284-8. PMID: 9751057

注文情報

製品名	カタログ #	単位	価格	数量	お気に入り
TLR4 抗体 (25)	sc-293072	200 µg/ml	$316.00
TLR4 (25): m-IgG Fc BP-HRP Bundle	sc-525846	200 µg Ab; 10 µg BP	$354.00
TLR4 抗体 (25) AC	sc-293072 AC	500 µg/ml, 25% agarose	$416.00
TLR4 抗体 (25) HRP	sc-293072 HRP	200 µg/ml	$316.00

Buongiorno. Sarebbe possibile avere questo anticorpo coniugato per fluorescenza su fettine tessuto incluso in paraffina?

質問者: Stefano

Thank you for your question. TLR4 Antibody (25): sc-293072 is not available with fluorescence conjugation. As possible alternatives we can offer TLR4 Antibody (HTA125): sc-13593, which is recommended for detection of TLR4/MD-2 complex of human origin by IP, IF, IHC(P) and FCM, or TLR4 Antibody (MTS510): sc-13591, which can be used for detection of TLR4/MD-2 complex of mouse, rat and human origin by IP, IF and FCM. Both are available with a variety of different conjugation options, which you can find on their individual product pages.

回答者: Tech Support Europe

投稿日: 2024-06-17

Is there any published paper about the IP application of this TLR4 antibody?

質問者: Summer

Thank you for your question. Please contact our Technical Service Department for more information.

回答者: Jiajun Z

投稿日: 2024-05-24

It is stated that this antibody is suitable for detecting TLR4 of mouse for WB. However, it is not stated that secondary antibody does not cross-react with mouse protein lysate. What is the recommended use of this antibody for WB from mouse tissue? 

質問者: Sb1927

Thank you for your question. We have performed proper testing on the secondary and can ensure you that the secondary will not cross react with mouse tissue . If you have more questions or need assistance please contact technical service . (800)-457-3801.

回答者: BlakeJ

投稿日: 2023-11-16

Is this antibody available? Is the agarose-conjugated antibody available as well?

質問者: yliu1

Thank you for your question. For accurate availability, pricing and shipping information regarding this biochemical product, please contact your local distributor for assistance.

回答者: Technical Service

投稿日: 2021-12-22

do you have a fluorescence tag version of TLR4 Antibody (25): sc-293072 available? or can you order a custom made conjugated version?

質問者: David Chau

Thank you for your question. TLR4 Antibody (HTA125): sc-13593 and TLR4 Antibody (MTS510): sc-13591 are available in fluorescence-conjugated versions. We do not offer fluorescence conjugates of TLR4 Antibody (25): sc-293072.

回答者: Tech Support Europe

投稿日: 2021-02-09

Is there any chances of double bands in the immunoblot of mouse peritoneal macrophages cells lysates?

質問者: Soumojit

Thank you for your question. We have not tested this antibody in that specific sample type. We recommend you refer to the product citations using this antibody as a reference. You can search for specific product citations by application, species and sample type using our Product Citation search on our website, or using the link below. https://www.scbt.com/scbt/misc/citationSearch.jsp

回答者: Technical Support

投稿日: 2019-08-17

What is the second antibody??

質問者: rlaalsgml36

Thank you for your question. You can find our available secondary antibodies here: https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4

回答者: Tech Service

投稿日: 2022-06-07

I didn't get positive result on mouse MC3T3E1 cells by using this TLR4 antibody with1:100 or 1:200 dilution,I was using 555 second antibody,what's the problem is?

質問者: tommy666

Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.

回答者: Technical Support

投稿日: 2022-11-11

TLR4抗体(25) への質問 - ページ 2

匿名によって、 5つ星のうち 5と評価されています。 Good antibody Works for WBIt works well for WB from both cells and tissues. It seems to work for IF in cells.

投稿日: 2025-06-30

hhskによって、 5つ星のうち 5と評価されています。功能很出色！我一个月前买了这个产品，感觉不错,效果很好，很好用，推荐大家都来用，试试这个无敌巨好用的抗体，赶紧来试试吧

投稿日: 2024-09-18

fisiojavによって、 5つ星のうち 5と評価されています。 Excellent results in WB from rat nervous systemWorks very well in 1:500 dilution, clear and strong signal at predicted molecular weight

投稿日: 2023-07-21

Simona PGによって、 5つ星のうち 1と評価されています。 It does not work in IF on FFPE tissue sectionsI used this Ab on paraffin embedded tissue sections at two dilutions (1:100 and 1:200) following the SC protocol for IF on such samples. I used a positive control, anti-MUC2, a sample previously and kindly provided by SC, which works very well. I do not exclude it may be a matter of low expression

投稿日: 2022-10-19

yakunによって、 5つ星のうち 5と評価されています。 The antibody is the best TLR4 antibody for WB.I have used the antibody 10years ago, and the result is very good, and I will share the information to my students.

投稿日: 2022-10-11

Isaiahによって、 5つ星のうち 5と評価されています。 Great for IHC / IFRequested a sample and used as a primary antibody for IHC / IF on human tissue after the HTA125 clone yielded no results. 1:50 (4 ug/mL) working concentration, goat-anti-mouse Dylight 488 as secondary antibody.

投稿日: 2022-09-29

C H Lによって、 5つ星のうち 5と評価されています。 Works wellSuccessfully used for primary detection and works as expected in Western blot!

投稿日: 2022-07-14

snoopyによって、 5つ星のうち 5と評価されています。特异性高蛋白免疫印迹实验中，多次使用该抗体，特异性高。由于组织样本表达丰富不同，需要根据情况适当调整上样量。

投稿日: 2022-07-04

TLR4抗体(25) のレビュー - ページ 2

TLR4抗体(25)は、 106件のレビューによって、 5つ星のうち 4.7と評価されています。

サンタクルーズバイオテクノロジーは生物医学研究市場向けの製品の開発において世界的リーダです。1-800-457-3801に電話してください.Copyright © 2007-2025 Santa Cruz Biotechnology, Inc.全著作権所有。”Santa Cruz Biotechnology”、及びSanta Cruz Biotechnology, Inc. ロゴ、 “Santa Cruz Animal Health”、 “San Juan Ranch”、“Supplement of Champions”、 San Juan Ranch ロゴ、 “UltraCruz”、 “ChemCruz”、 “ImmunoCruz”、 “ExactaCruz”、と “EZ Touch”はサンタクルーズバイオテクノロジーの登録商標です。商標はそれぞれの所有者に帰属します。

動物種	遺伝子名	遺伝子ID	染色体のロケーション	アイソフォーム (mRNA) アクセッション番号	タンパク質アクセッション番号	OMIM™ 番号
Human	TLR4	Entrez Gene ID: 7099	9q33.1	NM_003266, NM_138554, NM_138557, NM_138556	UniProt ID: O00206	611488
Mouse	Tlr4	Entrez Gene ID: 21898	4 C1	NM_021297	UniProt ID: Q9QUK6	N/A

TLR4抗体(25): sc-293072

TLR4抗体(25) 参考文献:

TLR4 抗体 (25) 規格

代替製品名: TLR4

クローン名: 25

種類: 一次抗体

Clonality: モノクローナル抗体

抗体種: IgG₁ kappa light chain

アミノ酸: 198-395

抗体部位: TLR4

エピトープ種: mouse

製品反応: TLR4

免疫種: mouse, rat, human, canine

他の哺乳類反応性: N/A

他の非哺乳類反応性: N/A

適用: WB, IP, IF, IHC(P)

包装: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

引用文献件数: 702 各種の科学出版物からの引用

TLR4 抗体 (25) 使用可能な共役/形式: AC: Agarose, HRP: Horseradish Peroxidase

好ましい TLR4 抗体は TLR4 抗体である (HTA125): sc-13593.

TLR4 抗体 (25)

TLR4 (25): m-IgG Fc BP-HRP Bundle

TLR4 抗体 (25) AC

TLR4 抗体 (25) HRP

Buongiorno. Sarebbe possibile avere questo anticorpo coniugato per fluorescenza su fettine tessuto incluso in paraffina?

Is there any published paper about the IP application of this TLR4 antibody?

It is stated that this antibody is suitable for detecting TLR4 of mouse for WB. However, it is not stated that secondary antibody does not cross-react with mouse protein lysate. What is the recommended use of this antibody for WB from mouse tissue?

Is this antibody available? Is the agarose-conjugated antibody available as well?

do you have a fluorescence tag version of TLR4 Antibody (25): sc-293072 available? or can you order a custom made conjugated version?

Is there any chances of double bands in the immunoblot of mouse peritoneal macrophages cells lysates?

What is the second antibody??

I didn't get positive result on mouse MC3T3E1 cells by using this TLR4 antibody with1:100 or 1:200 dilution,I was using 555 second antibody,what's the problem is?

TLR4抗体(25): sc-293072

TLR4抗体(25) 参考文献:

TLR4 抗体 (25) 規格

代替製品名: TLR4

クローン名: 25

種類: 一次抗体

Clonality: モノクローナル抗体

抗体種: IgG1 kappa light chain

アミノ酸: 198-395

抗体部位: TLR4

エピトープ種: mouse

製品反応: TLR4

免疫種: mouse, rat, human, canine

他の哺乳類反応性: N/A

他の非哺乳類反応性: N/A

適用: WB, IP, IF, IHC(P)

包装: Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

引用文献件数: 702 各種の科学出版物からの引用

TLR4 抗体 (25) 使用可能な共役/形式: AC: Agarose, HRP: Horseradish Peroxidase

好ましい TLR4 抗体は TLR4 抗体である (HTA125): sc-13593.

TLR4 抗体 (25)

TLR4 (25): m-IgG Fc BP-HRP Bundle

TLR4 抗体 (25) AC

TLR4 抗体 (25) HRP

Buongiorno. Sarebbe possibile avere questo anticorpo coniugato per fluorescenza su fettine tessuto incluso in paraffina?

Is there any published paper about the IP application of this TLR4 antibody?

It is stated that this antibody is suitable for detecting TLR4 of mouse for WB. However, it is not stated that secondary antibody does not cross-react with mouse protein lysate. What is the recommended use of this antibody for WB from mouse tissue?&nbsp;

Is this antibody available? Is the agarose-conjugated antibody available as well?

do you have a fluorescence tag version of TLR4 Antibody (25): sc-293072 available? or can you order a custom made conjugated version?

Is there any chances of double bands in the immunoblot of mouse peritoneal macrophages cells lysates?

What is the second antibody??

I didn't get positive result on mouse MC3T3E1 cells by using this TLR4 antibody with1:100 or 1:200 dilution,I was using 555 second antibody,what's the problem is?

抗体種: IgG₁ kappa light chain

包装: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

It is stated that this antibody is suitable for detecting TLR4 of mouse for WB. However, it is not stated that secondary antibody does not cross-react with mouse protein lysate. What is the recommended use of this antibody for WB from mouse tissue?