TFB2M Antibody (2E10): sc-517095

TFB2M Antibody (2E10) is a mouse monoclonal IgG1 kappa light chain antibody that detects TFB2M protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-TFB2M antibody (2E10) is available as a non-conjugated format. TFB2M, or transcription factor B2, mitochondrial, is a crucial 396 amino acid mitochondrial protein that plays a significant role in the regulation of mitochondrial gene expression. TFB2M belongs to the rRNA adenine N(6)-methyltransferase family and is responsible for the specific dimethylation of mitochondrial 12S rRNA at a conserved stem loop, a modification essential for proper assembly and function of mitochondrial ribosomes. This post-translational modification influences stability and translation efficiency of mitochondrial mRNA, impacting overall protein synthesis within mitochondria. TFB2M is expressed ubiquitously and is required for basal transcription of mitochondrial DNA, demonstrating importance in cellular energy metabolism and maintenance of mitochondrial function. While TFB2M exhibits less methyltransferase activity compared to TFB1M, TFB2M is more efficient in activating mitochondrial DNA transcription, highlighting a unique regulatory role in mitochondrial biology.

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TFB2M Antibody (2E10) References:

1. Mitochondrial transcription factors B1 and B2 activate transcription of human mtDNA.  |  Falkenberg, M., et al. 2002. Nat Genet. 31: 289-94. PMID: 12068295
2. Control of mitochondrial transcription specificity factors (TFB1M and TFB2M) by nuclear respiratory factors (NRF-1 and NRF-2) and PGC-1 family coactivators.  |  Gleyzer, N., et al. 2005. Mol Cell Biol. 25: 1354-66. PMID: 15684387
3. Aging impact on biochemical activities and gene expression of Drosophila melanogaster mitochondria.  |  Dubessay, P., et al. 2007. Biochimie. 89: 988-1001. PMID: 17524546
4. Relative abundance of the human mitochondrial transcription system and distinct roles for h-mtTFB1 and h-mtTFB2 in mitochondrial biogenesis and gene expression.  |  Cotney, J., et al. 2007. Nucleic Acids Res. 35: 4042-54. PMID: 17557812
5. Mitochondrial transcription factor A (TFAM) gene variation in Parkinson's disease.  |  Alvarez, V., et al. 2008. Neurosci Lett. 432: 79-82. PMID: 18248889
6. Mitochondrial transcription factor B2 is essential for metabolic function in Drosophila melanogaster development.  |  Adán, C., et al. 2008. J Biol Chem. 283: 12333-42. PMID: 18308726
7. Mutational screening of the mitochondrial transcription factors B1 and B2 (TFB1M and TFB2M) in Parkinson's disease.  |  Sánchez-Ferrero, E., et al. 2009. Parkinsonism Relat Disord. 15: 468-70. PMID: 18980857
8. Mitochondrial transcription factors TFA, TFB1 and TFB2: a search for DNA variants/haplotypes and the risk of cardiac hypertrophy.  |  Alonso-Montes, C., et al. 2008. Dis Markers. 25: 131-9. PMID: 19096125

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TFB2M Antibody (2E10) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-TFB2M Antibody (2E10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TFB2M Antibody (2E10) Specifications

Alternate Product Name: TFB2M

Product Clone Name: 2E10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 297-396

Epitope Target: TFB2M

Epitope Species: human

Product Reactivity/Detection: TFB2M

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for TFB2M Antibody (2E10): N/A